Efektivitas Pentagamavunon-0 (PGV-0) pada fase awal infeksi virus Dengue-2
Effectiveness of Pentagamavunon-0 (PGV-0) in the early phase infection of Dengue-2 virus
Abstract
Abstrak.Virus Dengue menginfeksi 50 sampai 100 juta orangper tahun, namun terapi yang spesifik atau obat antivirus yang efektif belum ditemukan. Kurkumin diketahui mampu melakukan penghambatan system ubiquitin-proteasome yang menyebabkan penurunan produksi salah satu jenis Flavivirus yaitu Japanese encephaitis. Modifikasi struktur kurkumin terbukti meningkatkan aktivitas biologisnya. Pentagamavunon-0 (PGV-0) diketahui memiliki aktifitas mirip atau bahkan lebih baik dari kurkumin. Tujuan dari penelitian ini adalah mengetahui pengaruh pemberian PGV-0 pada fase awal infeksi virus Dengue-2 (satu hari infeksi). Penelitian ini termasuk penelitian kuasi-eksperimental. Metode yang digunakan untuk deteksi virus Dengue-2 dalam penelitian ini adalah imunositokimia, setelah sebelumnya dilakukan uji sitotoksik PGV-0 terhadap sel vero. Hasil uji sitotoksik menunjukkan konsentrasi yang aman (tidak memberikan efek toksik) dari PGV-0 terhadap sel Vero adalah 4,44 μM. Hasil uji imunositokimia menunjukkan nilai rata-rata sel positif akibat infeksi Dengue-2 satu hari dengan perlakuan PGV-0 jauh lebih kecil (3.8 ± 3.89) dibandingkan kontrol positifnya (14.55 ± 7.25). Disimpulkan bahwa PGV-0 mampu menurunkan positive rate akibat infeksi Den-2 pada periode awal infeksi
References
2. Halstead SB. Dengue virus mosquito interactions, Annual Review of Entomology. 2008; 53: 273–91.
3. Harris E, Holden KL, Edgil D, Polacek C, Clyde K. Molecular biology of flaviviruses, Novartis Found Symp. 2006; 277: 23–39.
4. SoegijantoS, Darmowandowo W, Ginting AP, Yamanaka A. Serotype and Clinical Performance of Dengue Virus Infection on the Year 2009. Indones J.Trop Infec Disease.2010; 1(2): 55-5.
5. Crance JM, Natale S, Alain J, Daniel G. Interferon, ribavirin, 6-azauridine and glycyrrhizin: antiviral compounds active againts pathogenic flaviviruses. Antiviral Res.Elsevier. 2003; 58: 73-9.
6. Prusty BK, Das BC, Constitutive activation of transcription AP-1 in cervical cancer and suppression of human papillomavirus (HPV) transcription and AP-1 activity in Hela cells by curcumin. Int J Cancer. 2005; 113: 951–60.
7. Ramendra KS, Diwakar R, Dipti Y, Bhargava A, Balzarini J, De Clercq E. Synthesis, antibacterial and antiviral properties of curcumin bioconjugates bearing dipeptide, fatty acids and folic acid, European Journal of Medicinal Chemistry. 2010; 45: 1078–86.
8. Rai D, Yadav J, Balzarini E, De Clercq and Singh RK. Design and Development of Curcumin Bioconjugates as Antiviral Agent. 2008. [Cited 2011 Maret 16]. Available from http://nas.oxfordjournals.org/cgi/content/short/52/1/599.
9. Dutta K, Debapriya G and Anirban B. Curcumin Protects Neuronal Cells from Japanese Encephalitis Virus-Mediated Cell Death and also Inhibits Infective Viral Particle Formation by Dysregulation of Ubiquitin–Proteasome System.J. of NeuroimmunePhar. 2009; 4 (3): 328-37.
10. Sharma RA, Gescher AJ, Steward WP. Curcumin: The story so far. Eur. J. of Cancer. 2005; 41: 1955-68.
11. Sardjiman. Synthesis of Some New Series of Curcumin Analogues, Anti-oxidative, anti-inflamatory, antibacterial activities and qualitative-structure Activity Relationship [dissertation]. Yogyakarta: Gadjah Mada University; 2000.
12. Sardjiman SS, Reksohadiprodjo MS, Hakim L, Vand der Goot H, Timmerman H. “1,5-Diphenyl-1-4-pentadiene-3-ones and cyclic
analogues as antioxidative agents. Synthesis and structure-activity relationship. Eur. J. of Med.Chem. 1997; 32: 625-30.
13. Meiyanto E, Kurkumin sebagai obat kanker. Menelusuri mekanisme aksinya.Majalah Farmasi Indonesia. 1999; 10(4): 224 – 36.
14. Faujan NA, Noriham A, Norrakiah AS and Babji AS. Antioxidant Activity of Plants Methanolic Extracts Containing Phenolic Compounds. African J of Biotech. 2009; 3: 484-9.
15. Umniyati SR, Sutaryo, Wahyono D, Artama WT. Application of monoclonal antibody DSSC7 for early detection of dengue infection in blood smear preparation based on immunocytochemical streptavidin biotin peroxidase complex assay. In: Int. Joint. Symp. Frontier Sciences from gene to application. Faculty of Medicine. Universitas Gadjah Mada; 2008.
16. Zandia K, Ramedani E, Mohammadi K, Tajbakhsh S, Deilami I, Rastian Z et al.Evaluation of Antiviral Activities of Curcumin Derivatives against HSV-1 in Vero Cell Line. Natural Product Communications (NPC). 2010; 5(12): 1935 – 38.
17. Umniyati SR. Teknik Imunositokimia dengan Antibodi Monoklonal DSSC7 untuk Kajian Patogenesis Infeksi dan Penularan Transovarial Virus Dengue serta Surveilansi Virologis Vektor Dengue[disertasi]. Yogyakarta: Universitas Gadjah Mada; 2009.
18. Yennamali R, Subbarao N, Kampmann T, McGeary RP, Young PR, Kobe B, Identification of novet target sites and an inhibitor of the dengue virus E protein, J. Comput Aided Mol. 2009; 23: 333-41.
19. Noble CG, Chen YL, Dong H, Gu F, Lim SP, Schul W, et al., Strategies for development of dengue virus inhibitors. Antiviral Research. 2010;85: 450-62.
20. Qamar MT, Mumtaz A, Naseem R, Ali A, Fatima T, Jabbar T,et al., Molecular Docking Based Screening of Plant Flavonoids as Dengue NS1 Inhibitors. Bioinformation. 2014; 10(7): 460–465.
21. Yuniarti N, Martono S, Pengaruh 1,5-bis(4’-hidroksi-3’-metoksifenil)-1,4-pentadien-3-on dan kurkumin pada aktivitas enzim glutation S-transferase paru tikus. Jurnal Farmasi Indonesia. 2006; 3(1): 44 – 52
22. Syamsudin. Pengembangan Metode Analisis Pentagamavunon-0 dalam Cairan Biologis secara Kromatografi Cair Kinerja Tinggi [tesis]. Yogyakarta: Fakultas Farmasi, Universitas Gadjah Mada; 2003.
Copyright (c) 2014 The Authors
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
