Mosquito Larvacidal Activity of Zingiber montanum Rhizome Extract Against  Aedes aegypti larvae

Dwi Sulistia Ningrum; Siwi Pramatama Mars Wijayanti; Kuswanto Kuswanto

doi:10.22435/blb.v15i1.1546

Dwi Sulistia Ningrum Department of Public Health, Faculty of Health Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno Karangwangkal, Purwokerto Utara, Jawa Tengah, Indonesia
Siwi Pramatama Mars Wijayanti Department of Public Health, Faculty of Health Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno Karangwangkal, Purwokerto Utara, Jawa Tengah, Indonesia
Kuswanto Kuswanto Department of Public Health, Faculty of Health Sciences, Jenderal Soedirman University, Jl. Dr. Soeparno Karangwangkal, Purwokerto Utara, Jawa Tengah, Indonesia

DOI: https://doi.org/10.22435/blb.v15i1.1546

Keywords: Dengue, Aedes aegypti, Zingiber, biolarvacide

Abstract

Aedes aegypti is the main vector which transmits Dengue virus as causing agent of Dengue Haemmorhagic Fever (DHF). Chemical control of Ae. aegypti have an impact on the environment and humans, also burden a high cost. One of the efforts to reduce the negative impact of synthetic insecticide, which is to find out alternative natural insecticide from plant-based insecticides. The purpose of this research is to determine the killing power of the rhizome bangle extract to Ae. aegypti larvae. This research was a quasi-experimental design with post test only control group design. The concentration of extract rhizome bangle used were 0%; 0.125%; 0.25%; 0.5%; 0.75% and 1%. The mosquito sample used in this study were instar III of Ae. aegypti larvae as many as 600 larvae. Data analysis used univariate and bivariate (probit and Kruskal Wallis test). The results showed that there was an effect of the concentration of extract rhizome bangle against the mortality of Ae. aegypti larvae with p=0,002 (p<0,05). Extract rhizome bangle effectively killed Ae. aegypti larvae with LC₅₀ and LC₉₀ were 0.148 % and 0.338 %, with the most effective concentration is 1%. Based on this research, extract rhizome bangle has a larvicidal effect on Ae. aegypti, so it can be used as an alternative method to minimize the usage of chemical larvicides that easily applied by the community.

References

1. Alvarado-Castro V, Paredes-Solís S, Nava-Aguilera E, Morales-Pérez A, Alarcón-Morales L, Balderas-Vargas NA, et al. Assessing the effects of interventions for Aedes aegypti control: systematic review and meta-analysis of cluster randomized controlled trials. BMC public health. 2017;17(Suppl 1):384. doi: 10.1186/s12889-017-4290-z.

2. Benelli G, Jeffries CL, Walker T. Biological control of mosquito vectors: past, present, and future. Insects. 2016;7(4):52. doi: 10.3390/insects7040052.

3. Macias VM, Ohm JR, Rasgon JL. Gene drive for mosquito control: where did it come from and where are we headed?. International Journal of Environmental Research and Public Health. 2017;14(9):1006. doi: 10.3390/ijerph14091006.

4. Rodrigo C, Fernando SD, Rajapakse S, Weeratunga P. Control methods for Aedes albopictus and Aedes aegypti. Cochrane Database of Systematic Reviews. 2017(8):CD012759. doi: 10.1002/14651858.CD012759.

5. Abeyasuriya KGTN, Nugapola NWNP, Perera MDB, Karunaratne WAIP, Kurunaratne SHPP. Effect of dengue mosquito control insecticide thermal fogging on non-target insects. International Journal of Tropical Insect Science.2016;37(1):11-8. doi: 10.1017/S1742758416000254.

6. Ramkumar G, Karthi S, Muthusamy R, Suganya P, Natarajan D, Kweka EJ, et al. Mosquitocidal effect of Glycosmis pentaphylla leaf extracts against three mosquito species (diptera: Culicidae). PLOS ONE. 2016;11(7):e0158088. doi: 10.1371/journal.pone.0158088.

7. Pavela R. History, Presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects - a review. Plant Protect Sci.2016;52(4):229-41. doi:10.17221/31/2016-PPS.

8. Yousaf A, Zuharah WF. Lethal response of the dengue vectors to the plant extracts from family Anacardiaceae. Asian Pacific Journal of Tropical Biomedicine. 2015;5(10):812-8. doi: 10.1016/j.apjtb.2015.05.016.

9. Soleimani-Ahmadi M, Abtahi SM, Madani A, Paksa A, Abadi YS, Gorouhi MA, et al. Phytochemical profile and mosquito larvicidal activity of the essential oil from aerial parts of Satureja bachtiarica bunge against malaria and lymphatic filariasis vectors. Journal of Essential Oil Bearing Plants. 2017;20(2):328-36. doi:10.1080/0972060X.2017.1305919.

10. Govindarajan M, Rajeswary M, Senthilmurugan S, Vijayan P, Alharbi NS, Kadaikunnan S, et al. Larvicidal activity of the essential oil from Amomum subulatum Roxb. (Zingiberaceae) against Anopheles subpictus, Aedes albopictus and Culex tritaeniorhynchus (Diptera: Culicidae), and non-target impact on four mosquito natural enemies. Physiological and Molecular Plant Pathology. 2018;101:219-24. doi: 10.1016/j.pmpp.2017.01.003.

11. Rajeswary M, Govindarajan M, Alharbi NS, Kadaikunnan S, Khaled JM, Benelli G. Zingiber cernuum (Zingiberaceae) essential oil as effective larvicide and oviposition deterrent on six mosquito vectors, with little non-target toxicity on four aquatic mosquito predators. Environmental science and pollution research international. 2018;25(11):10307-16. doi: 10.1007/s11356-017-9093-3.

12. Singh Cb, Manglembi N, Swapana M, Chanu Sb. Ethnobotany, phytochemistry and pharmacology of Zingiber cassumunar Roxb. (Zingiberaceae). Journal of Pharmacognosy and Phytochemistry.2015;4(1):1-6. Available from: http://www.phytojournal.com/archives/2015/vol4issue1/PartA/3.1-921.pdf.

13. Sanatombi R, Sanatombi K. Biotechnology of Zingiber montanum (Koenig) link ex A. Dietr.: a review. Journal of Applied Research on Medicinal and Aromatic Plants. 2017;4:1-4. doi: 10.1016/j.jarmap.2016.09.001.

14. Kasarkar A, Kulkarni D, Dhudade P, Sabu M. New report on Zingiber montanum (K.D. Koenig) link. from Kudal, Dist. Sindhudurg, (MS) India. Bioscience Discovery. 2017;8(2):270-3. Available from: https://jbsd.in/Vol%208%20No%202/Abhijeet270-273.pdf.

15. Perumalsamy H, Jang MJ, Kim JR, Murugan K, Ahn YJ. Larvicidal activity and possible mode of action of four flavonoids and two fatty acids identified in Millettia pinnata seed toward three mosquito species. Parasites & Vectors.2015;8:237. doi: 10.1186/s13071-015-0848-8.

16. Zhang QW, Lin LG, Ye WC. Techniques for extraction and isolation of natural products: a comprehensive review. Chin Med. 2018;13:20. doi: 10.1186/s13020-018-0177-x.

17. El-Akhal F, Guemmouh R, Zoubi YE, Lalami AEO. Larvicidal activity of Nerium oleander against larvae west nile vector mosquito Culex pipiens (Diptera: Culicidae). Journal of Parasitology Research. 2015;2015:943060. doi: 10.1155/2015/943060.

18. Jawale CS. Larvicidal activity of some saponin containing plants against the dengue vector Aedes aegypti. Trends in Biotechnology Research An International Peer-Reviewed Journal.2014;3(1):1-11. Available from: https://pdfs.semanticscholar.org/8e8f/03ff74fd3f64ab14a822bc91109fa65bab48.pdf.

19. Kim SI, Ahn YJ. Larvicidal activity of lignans and alkaloid identified in Zanthoxylum piperitum bark toward insecticide-susceptible and wild Culex pipiens pallens and Aedes aegypti. Parasit & Vectors. 2017;10:221. doi: 10.1186/s13071-017-2154-0.

20. Liu ZL, Liu QZ, Du SS, Deng ZW. Mosquito larvicidal activity of alkaloids and limonoids derived from Evodia rutaecarpa unripe fruits against Aedes albopictus (Diptera: Culicidae). Parasitology Research.2012;111(3):991-6. doi: 10.1007/s00436-012-2923-9.

21. Pandey A, Pandey UK, Shrivastava PK. Studies on mosquito larvicidal efficacy of indigenous plant extracts. Journal of Ecophysiology and Occupational Health.2010;10(3-4):223-7. Available from: http://www.informaticsjournals.com/index.php/JEOH/article/view/18345/15325.