Uji Diagnostik Filariasis Menggunakan Rapid Diagnostic Test (RDT) Brugia malayi  terhadap Pemeriksaan Mikroskopis di Desa Buntoi Kabupaten Gunung Mas, Provinsi Kalimantan Tengah

Gadis Rinaty Susanty; Hernayanti Hernayanti; Dwi Sarwani Sri Rejeki

doi:10.22435/blb.v17i2.4461

Gadis Rinaty Susanty Universitas Jenderal Soedirman, Jalan Dr. Soeparno, Karangwangkal, Karang Bawang, Grendeng, Kecamatan Purwokerto Utara, Purwokerto, Jawa Tengah, Indonesia
Hernayanti Hernayanti Universitas Jenderal Soedirman, Jalan Dr. Soeparno, Karangwangkal, Karang Bawang, Grendeng, Kecamatan Purwokerto Utara, Purwokerto, Jawa Tengah, Indonesia
Dwi Sarwani Sri Rejeki Universitas Jenderal Soedirman, Jalan Dr. Soeparno, Karangwangkal, Karang Bawang, Grendeng, Kecamatan Purwokerto Utara, Purwokerto, Jawa Tengah, Indonesia

DOI: https://doi.org/10.22435/blb.v17i2.4461

Keywords: diagnostic test, filariasis, Brugia malayi Rapid Diagnostic Test, finger blood survey

Abstract

Gunung Mas Regency, Central Kalimantan Province is one of the endemic filariasis areas with Microfilaria rate of 3.4%. One of the efforts made to control this problem is Mass Drug Administration once a year for 5 years. Currently, the Rapid Diagnostic Test (RDT) method is being developed, a quick and easy diagnostic technique to detect the presence of parasites in the patient's body. This study aims to determine the results of the filariasis diagnostic test using the Brugia malayi RDT on the microscopic examination in Buntoi Village, Gunung Mas Regency, Central Kalimantan Province. This research is a descriptive study with a cross-sectional approach with the research subjects all residents of Buntoi Village with inclusion and exclusion criteria totaling 161 samples. Collecting data was carried out by examination and interviews with questionnaires. Data analysis by calculating the microfilaria rate, sensitivity and specificity and calculating the frequency distribution of research variables. Data is presented in percentage form and displayed in tabular form. The results of the diagnostic study of B. malayi RDT and the microscopic examination were the same, i.e all were negative and no microfilariae were found. The diagnostic test for filariasis RDT Brugia malayi on microscopic examination (SDJ) obtained 0% sensitivity, 100% specificity, 0% Positive Predictive Value and 100% Negative Predictive Value. The level of public knowledge about filariasis includes 61% good category, knowledge of MDA 40% good category and knowledge about prevention of filariasis in good category 53%.

References

1. Diwakar DK, Wadhwani N, Prasad N, Gupta A. Primary retroperitoneal filariasis: a common disease of tropics with uncommon presentation and review of literature. BMJ Case Rep. 2018;2018:1-3. doi:10.1136/bcr-2018-226217.

2. Lourens GB, Farrell DK. Lymphatic filariasis. Nurs Clin North Am. 2019;25(2):181-92. doi: 10.1016/j.cnur.2019.02.007.

3. Deshpande A, Miller-Petrie MK, Johnson KB, Abdoli A, Abrigo MRM, Adekanmbi V, et al. The global distribution of lymphatic filariasis, 2000–18: a geospatial analysis. Lancet Glob Heal. 2020;8(9):e1186-e94.
doi:10.1016/S2214-109X(20)30286-2.

4. Lee J, Ryu JS. Current status of parasite infections in Indonesia: a literature review. Korean J Parasitol. 2019;57(4):329-39. doi:10.3347/kjp.2019.57.4.329.

5. Irawan AS, Boesri H, Nugroho SS. Program nasional untuk eliminasi filariasis limfatik: studi kasus di Kabupaten Pekalongan, Jawa Tengah. Vektora J Vektor dan Reserv Penyakit. 2018;10(2):95-102.
doi:10.22435/vk.v10i2.1057.

6. Santoso, Yahya, Supranelfy Y, Suryaningtyas NH, Taviv Y, et al. Risk of recrudescence of lymphatic filariasis after post-MDA surveillance in Brugia malayi endemic Belitung District, Indonesia. Korean J Parasitol. 2020;58(6):627-34.
doi:10.3347/kjp.2020.58.6.627.

7. WHO. Guideline: alternative mass drug administration regimens to eliminate lymphatic filariasis. Geneva: World Health Organization; 2017.

8. Noordin R, Itoh M, Kimura E, Rahman RA, Ravindran B, Mahmud R, et al. Multicentre evaluations of two new rapid IgG4 tests (WB rapid and panLF rapid) for detection of lymphatic filariasis. Filaria J. 2007;6(9):1-4. doi:10.1186/1475-2883-6-9.

9. Putri DF. Deteksi IgG4 antifilaria menggunakan antigen rekombinan Bm14 untuk diagnosis filariasis limfatik di Indonesia. J Ilmu Kedokt dan Kesehat. 2018;5(4):294-304. doi:10.33024/.v5i4.973.

10. Kumalasari TN. Sensitivitas dan Spesifisitas metode Brugia rapid test pada pemeriksaan Brugia malayi. Biomed J Indones J Biomedik Fak Kedokt Univ Sriwij. 2019;5(2):62-71. doi:10.32539/bji.v5i1.7983.

11. Dinas Kesehatan Provinsi Kalimantan Tengah. Laporan program eliminasi filariasis di Provinsi Kalimantan Tengah. Palangkaraya: Dinas Kesehatan Provinsi Kalimantan Tengah; 2002.

12. Solikah MP, Wijayanti SPM, Rujito L. Efektivitas pengobatan massal filariasis setelah empat tahun pengobatan massal di Desa Ploso, Demak, Jawa Tengah. Balaba J Litbang Pengendali Penyakit Bersumber Binatang Banjarnegara. 2021;17(1):93-106.
doi:10.22435/blb.v17i1.4096.

13. Juhairiyah J, Fakhrizal D, Hidayat S, Indriyati L, Hairani B. Kepatuhan masyarakat minum obat pencegah massal filariasis (kakgajah): studi kasus Desa Bilas, Kabupaten Tabalong. J Vektor Penyakit. 2019;13(1):49-58.
doi:10.22435/vektorp.v13i1.956.

14. Biritwum NK, Frempong KK, Verver S, Odoom S, Alomatu B, Kontoroupis OP, et al. Progress towards lymphatic filariasis elimination in Ghana from 2000-2016: Analysis of microfilaria prevalence data from 430 communities. PLoS Negl Trop Dis. 2019;13(8):1-15.
doi: 10.1371/journal.pntd.0007115.

15. Manolas RK, Kama M, Rainima-Qaniuci M, Bechu VD, Tuibeqa S, Winston MV, et al. Lymphatic filariasis in Fiji: progress towards elimination, 1997–2007. Trop Med Health. 2020;48(88):1-12.
doi:10.1186/s41182-020-00245-4.

16. Lau CL, Sheel M, Gass K, Fuimaono S, David MC, Won KY, Sheridan S, et al. Potential strategies for strengthening surveillance of lymphatic filariasis in American Samoa after mass drug administration: reducing ŉumber needed to test’ by targeting older age groups, hotspots, and household members of infected persons. PLoS Negl Trop Dis. 2020;14(12):1-28. doi:10.1371/journal.pntd.0008916.

17. Aye NN, Lin Z, Lon KN, Linn NYY, Nwe TW, Mon KM, et al. Mapping and modelling the impact of mass drug adminstration on filariasis prevalence in Myanmar. Infect Dis Poverty. 2018;7(56):1-11. doi:10.1186/s40249-018-0420-9.

18. Supali T, Djuardi Y, Lomiga A, Linda SN, Iskandar E, Goss CW, et al. Comparison of the impact of annual and semiannual mass drug administration on lymphatic filariasis prevalence in Flores Island, Indonesia. Am J Trop Med Hyg. 2019;100(2):336-43. doi:10.4269/ajtmh.18-0570.

19. Rahmah, Taniawati S, Shenoy RH, Lim BH, Kumaraswami V, Anuar AK, et al. Specificity and sensitivity of a rapid dipstick test (Brugia rapid) in the detection of Brugia malayi infection. Trans R Soc Trop Med Hyg. 2001;95(6):601-4.
doi: 10.1016/s0035-9203(01)90091-4.

20. Rahmah N, Shenoy RK, Nutman TB, Weiss N, Gilmour K, Maizels RM, et al. Multicentre laboratory evaluation of Brugia rapid dipstick test for detection of brugian filariasis. Trop Med Int Health. 2003:8(10):895-900.

21. Ubhayawardana NL, Ghouse S, Abeyewickreme W. A single PCR assay to detect and differentiate Wuchereria bancrofti and Brugia malayi in expatriates and locals with filariasis in Sri Lanka. Int J Infect Dis. 2020;101:428-9.
doi: 10.1016/j.ijid.2020.09.1124.

22. Melchers NVSV, Coffeng LE, De Vlas SJ, Stolk WA. Standardisation of lymphatic filariasis microfilaraemia prevalence estimates based on different diagnostic methods: a systematic review and meta-analysis. Parasites & Vectors. 2020;13(302):1-9.
doi:10.1186/s13071-020-04144-9.

23. Nasution SF, Ekawati E. Prevalensi mikrofilaria setelah pengobatan masal 4 tahun di wilayah Kampung Sawah, Kecamatan Ciputat, Tangerang Selatan. Al-Kauniyah J Biol. 2013;6(2):113-9.
doi:10.15408/al-kauniyah.v6i2.2839.

24. Puspaningrum LR, Sunarsih E. Risk factor analysis: filariasis events in Sembawa Public Health Center area at Banyuasin District in 2019. Adv Heal Sci Res. 2020;25(2):136-141. doi:10.2991/ahsr.k.200612.017.

25. Amalia IS, Annashr NN. Faktor sosiodemografi dan perilaku yang berhubungan dengan kejadian filariasis di Kabupaten Kuningan. J Kampus STIKES YPIB Majalengka. 2018;6(1):1-19.

26. Yacob Y, Nurramadhan N, Dewi RM. Deteksi antibodi IgG4 dengan teknik elisa untuk evaluasi transmisi filariasis pasca POPM pada masyarakat di Kabupaten Aceh Jaya. J Biotek Medisiana Indones. 2018;7(2):97-102.
doi: 10.22435/jbmi.v7i2.2381.

27. Annashr NN, Rahmadi FM. Hubungan pengetahuan dan sikap dengan perilaku pencegahan filariasis di Kecamatan Cilimus Kabupaten Kuningan. Aspirator - J Penyakit Tular Vektor. 2021;13(1):23-36.
doi:10.22435/asp.v13i1.4621.

28. Arini, Sawaraswati LD, Ginandjar P, Martini. Prevalensi filariasis dan gambaran pengobatan masal di wilayah kerja Puskesmas Jembatan Mas Kabupaten Batang Hari. J Kesehat Masy. 2018;6(1):178-90.