Identifikasi Mikroba Simplisia Herba Ekinase  (Echinacea Purpurea) dan Daun Kepel (Stelechocarpus Burahol) Pada Empat Variasi Kemasan dengan Rapid tm  One System

Aniska Novita Sari; Inge Oktaviani; Fitriana Fitriana; Dyah Subositi

doi:10.22435/bpk.v48i1.2653

Aniska Novita Sari Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional
Inge Oktaviani Indah Kiat Plup dan Paper
Fitriana Fitriana Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional
Dyah Subositi Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

DOI: https://doi.org/10.22435/bpk.v48i1.2653

Keywords: Echinacea purpurea, Stelechocarpus burahol, Variation of packaging, Vacuum plastic, Paper, Aerob bacteria

Abstract

The lifestyle back to nature encourages the development of herbal research in the world. The quality of herbal products is influenced by several factors including the packaging process and the types of packaging material. The objective of this study was to identify of microbes in Echinacea purpurea and Stelechocarpus burahol which were packaged in 4 packaging material types (Paper, Vacuum plastic, plastic-coated paper, plastic coated paper). Sample were collected after being stored for 2 months in WKJ (Wisata Kunjungan Jamu) Kalibakung. Microbes were grown in PCA (Plate Count Agar) media, observation of colony morphology, microbial purification in NA (Nutrient Agar) media, gram staining, Catalase Test, Oxidase Test, and Identification Test using the RapID TM ONE System. The results showed that packaging material variations had an effect in microbial species grown in E. purpurea and S. burahol herbs simplisia. Bacteria on simplisia of E. purpurea packed using paper material were Corynebacterium jeikeium and Leminorella richardii ; Vacuum Plastic: Enterobacter cloacae; plastic-coated paper: E. aerogenes and Acinetobacter calcoaceticus; and paper plastic: A. calcoaceticus . Bacteria in simplisia of S. burahol leaves packed using paper material: Shigella sp., E. aerogenes, and Burkholderia cepacia; Vacuum plastic: A. calcoaceticus, Leminorella richardii and arabic; plastic- coated paper: A. calcoaceticus ; and paper coated plastic: L. richardii . There were no microbes species required by BPOM found in simplisia that packaged using various types of packaging materials. Recommendation for simplisia packaging material is using vacuum plastic.

Keywords : Echinacea purpurea, Stelechocarpus burahol, microbes, packaging material types

Abstrak

Gaya hidup kembali ke alam mendorong pengembangan penelitian herbal di dunia. Kualitas produk herbal dipengaruhi oleh beberapa faktor termasuk proses pengemasan dan jenis bahan kemasan. Tujuan dari penelitian ini adalah untuk mengidentifikasi mikroba dalam simplisia kering Echinacea purpurea dan Stelechocarpus burahol yang dikemas dalam 4 jenis bahan kemasan (Kertas, plastik vakum, kertas berlapis plastik, kertas dilapisi plastik). Sampel dikumpulkan setelah disimpan selama 2 bulan di WKJ (Wisata Kunjungan Jamu) Kalibakung. Mikroba ditanam dalam media PCA (Plate Count Agar), dilakukan pengamatan morfologi koloni, pemurnian mikroba dalam media NA (Nutrient Agar), pewarnaan gram, Uji Katalase, Tes Oksidase, dan Uji Identifikasi menggunakan RapID TM ONE System. Hasil penelitian menunjukkan bahwa variasi bahan kemasan memiliki efek pada spesies mikroba yang tumbuh pada simplisia E. purpurea dan S. burahol. Bakteri pada simplisia E. purpurea yang dikemas menggunakan bahan kertas adalah Corynebacterium jeikeium dan Leminorella richardii; Plastik Vakum: Enterobacter cloacae; kertas berlapis plastik: E. aerogenes dan Acinetobacter calcoaceticus; dan kertas plastik: A. calcoaceticus. Bakteri dalam simplisia daun S. burahol dikemas menggunakan bahan kertas: Shigella sp., E. aerogenes, dan Burkholderia cepacia; Plastik vakum: A. calcoaceticus, Leminorella richardii dan A. calcoaceticus; kertas berlapis plastik: A. calcoaceticus; dan plastik berlapis kertas: L. richardii. Tidak ada spesies mikroba yang disyaratkan oleh BPOM ditemukan pada simplisia yang dikemas menggunakan berbagai jenis bahan kemasan. Rekomendasi untuk bahan kemasan simplisia adalah menggunakan plastik vakum.

Kata kunci: Echinacea purpurea, Stelechocarpus burahol, mikroba, jenis bahan kemasan

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