Efektivitas Stabilitas Bacillus thuringiensis H-14 Isolat Salatiga Sediaan Bubuk Terhadap Jentik Anopheles SPP. di Kabupaten Purworejo
Abstract
Abstract
Malaria is a vector-borne disease that is still a health problem in Indonesia. Control of vector mosquito larvae using Bacillus thuringiensis (Bt H-14) is an alternative biolarvicide. B2P2VRP has developed Salatiga Bt H-14 isolate in powder preparation which need to be tested for effectiveness stability. This study aims to determine the effectiveness stability of Salatiga powder Bt H-14 isolate against larvae of Anopheles spp. Preparation of Salatiga powder Bt H-14 isolate and its bioassay test against larvae of Anopheles aconitus were carried out at laboratory of the B2P2VRP. The stability test was carried out at the laboratory of UII Yogyakarta. The stability effectiveness test was carried out in the field in Purworejo using Anopheles spp. larvae taken in the field. The bioassay test (LC90 ) at initial and after stored at 54 o C ± 2 o C for two weeks were 6.485 ppm and 13.009 ppm, respectively. The study showed the effectiveness of stability decreased on days 2 and 3 (<70%) at dose of 26,018 ppm because of rain. Statistical test showed a difference in the effectiveness of reducing larvae mortality at days 2 and 3. The study confirmed the reduction of effectiveness of Salatiga powder Bt H-14 Isolate after stability test.
Keywords: Anopheles larvae, Bacillus thuringiensis, effectiveness, Purworejo, stability
Abstrak
Malaria merupakan salah satu penyakit tular vektor yang masih menjadi masalah kesehatan di Indonesia. Pengendalian jentik nyamuk vektor menggunakan Bacillus thuringiensis (Bt H-14) merupakan salah satu biolarvisida alternatif. B2P2VRP telah mengembangkan Bt H-14 isolat Salatiga sediaan bubuk maka perlu diuji stabilitas. Penelitian ini bertujuan menentukan efektivitas stabilitas Bt H-14 isolat Salatiga sediaan bubuk terhadap jentik Anopheles spp di lapangan. Pembuatan Bt H-14 Isolat Salatiga sediaan bubuk dan uji bioassay terhadap jentik Anopheles aconitus dilakukan di laboratorium B2P2VRP. Uji stabilitas dilakukan di laboratorium UII Yogyakarta. Uji efektivitas stabilitas dilakukan di Purworejo menggunakan jentik Anopheles spp. yang diambil di lapangan. Hasil uji bioassay (LC90 ) sebelum dan setelah penyimpanan suhu 54 o C ± 2 o C selama 2 minggu adalah 6,485 ppm dan 13,009 ppm. Hasil menunjukkan efektivitas stabilitas menurun pada hari ke 2 dan 3 (<70%) pada dosis 26,018 ppm adanya pengaruh hujan. Uji statistik menunjukkan adanya perbedaan efektivitas penurunan kematian jentik saat hari ke 2 dan 3. Hasil penelitian menunjukkan adanya penurunan efektivitas dari Bt H-14 Isolat Salatiga sediaan bubuk setelah dilakukan uji stabilitas.
Kata kunci : Anopheles larvae, Bacillus thuringiensis, efektivitas, Purworejo, stabilitas
References
Kementerian Kesehatan RI. Profil Kesehatan Indonesia Tahun 2017 [Internet]. 2018. Available from: website: http://www.kemkes. go.id
Dinas Kesehatan Provinsi Jawa Tengah. Profil Kesehatan Provinsi Jawa Tengah Tahun 2017. Semarang : Dinas Kesehatan Provinsi Jawa Tengah; 2018.
Dinas Kesehatan Kabupaten Purworejo. Profil kesehatan 2016. Purworejo : Dinas Kesehatan Kabupaten Purworejo; 2017.
Yuantari MGC, Widianarko B, Sunoko HR. Analisis Risiko Pajanan Pestisida Terhadap Kesehatan Petani. Jurnal Kesehat Masyarakat. 2015;10(2):239–45.
Liu N. Insecticide Resistance in Mosquitoes: Impact, Mechanisms, and Research Directions. Annual Review of Entomology. 2015;60(1):537–59.
Osman GEH, Already R, Assaeedi ASA, Organji SR, El-Ghareeb D, Abulreesh HH, et al. Bioinsecticide Bacillus thuringiensis a comprehensive review. Egyptian Journal of Biological Pest Control. 2015;25(1):271–88.
Zhang Q, Hua G, Adang MJ. Effect and mechanisms of Bacillus thuringiensis crystal toxins for mosquito larvae. Insect Science. 2016;24(5):714–29.
Blondine Ch.p, Widyastuti U, Widiarti, Sukarno, Subiantoro. Uji Serologi Isolat Bacillus thuringiensis dan Patogenisitasnya Terhadap Jentik Nyamuk Vektor. Buletin Penelitian Kesehatan. 1999;26(2&3):91–8.
Anggraeni YM, Rahardianingtyas E, Wianto R. Bioassay Bacillus thuringiensis H-14 Isolat Salatiga Sediaan Bubuk dan Cair Terhadap Culex quinquefasciatus. Vektora. 2015;7(2):51–6.
Mpofu M, Becker P, Mudambo K, De Jager C. Field effectiveness of microbial larvicides on mosquito larvae in malaria areas of Botswana and Zimbabwe. Malaria Journal. 2016;15(1):1–8.
Tamilselvan S, Manonmani AM, Jambulingam P. Fly ash-based water dispersible powder formulation of Bacillus thuringiensis var. Israelensis: Development & laboratory evaluation against mosquito immatures. Indian J Med Res. 2017;146(6):714–21
Wibowo CI. Efektivitas Bacillus thuringiensis dalam Pengendalian Larva Nyamuk Anopheles sp. Biosfera. 2017;34(1):39
Sayuti NA. Formulasi dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Daun Ketepeng Cina (Cassia alata L .). Jurnal Kefarmasian Indonesia. 2015;5(2):74–82.
WHO, FAO. Manual on Development and Use of FAO and WHO Specifications for Pesticides. 2010.
Dambach P, Louis VR, Kaiser A, Ouedraogo S, Sié A, Sauerborn R, et al. Efficacy of Bacillus thuringiensis var. israelensis against malaria mosquitoes in northwestern Burkina Faso. Parasites and Vectors. 2014;7(371):1– 8.
World Health Organization. Guidelines for laboratory and field testing of mosquito larvicides. World Health Organization Communicable Disease Control, Prevention and Eradication Who Pesticide Evaluation Scheme. 2005. 1–41 p
Moustafa MAM, Saleh MA, Ateya IR, Kandil MA. Influence of some environmental conditions on stability and activity of Bacillus thuringiensis formulations against the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). Egypt J Biol Pest Control. 2018;28(61):1–7
Zaini AN, Gozali D. Pengaruh Suhu Terhadap Stabilitas Obat Sediaan Suspensi. Jurnal Farmaka. 2016;14(2):1–11.
Brar SK, Verma M, Tyagi RD, Valéro JR, Surampalli RY. Starch industry wastewaterbased stable Bacillus thuringiensis liquid formulations. J Econ Entomol. 2005;98(6):1890–8.
Sorokulova IB, Krumnow AA, Pathirana S, Mandell AJ, Vodyanoy V. Novel Methods for Storage Stability and Release of Bacillus Spores. Biotechnol Prog. 2008;24(5):1147– 53.
Palma L, Muñoz D, Berry C, Murillo J, Caballero P. Bacillus thuringiensis Toxins: An Overview of Their Biocidal Activity. Toxins (Basel). 2014;6(12):3296–325.
Zaini AN, Gozali D. Pengaruh Suhu Terhadap Stabilitas Obat Sediaan Suspensi. Jurnal Farmaka. 2016;14(2):1–11.
Wardani AT, Anggraeni YM, Nugroho A, Wianto R, Rahardianingtyas E. Pengaruh kondisi lingkungan terhadap efektivitas Bacillus thuringiensis H-14 Isolat Salatiga sediaan serbuk untuk pengendalian jentik Anopheles spp di Kabupaten Kulon Progo. Vektora. 2019;11(2):103–10.
Uragayala S, Kamaraju R, Tiwari S, Ghosh SK, Valecha N. Field testing & evaluation of the efficacy & duration of effectiveness of a biolarvacide, Bactivec® SC (Bacillus thuringiensis var. israelensis SH-14) in Bengaluru, India. indian Journal of Medical Research [Internet]. 2018;147:299–307. Available from: http://www.ncbi.nlm.nih.gov/pubmed/23144490
Ma M, Huang M, Leng P. Abundance and distribution of immature mosquitoes in urban rivers proximate to their larval habitats. Acta Tropica [Internet]. 2016;163:121–9. Available from: http://dx.doi.org/10.1016/j.actatropica.2016.08.010
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