Efek Protektif Sinergis Kombinasi Minyak Jintan Hitam (Nigella sativa) Komersial dan Madu terhadap Nefrotoksisitas pada Tikus akibat Cisplatin

Abstract

Abstrak Cisplatin (CP) merupakan agen kemoterapi yang banyak digunakan dan menyebabkan nefrotoksisitas. Minyak jintan hitam (MJH) dan madu (M) diketahui memiliki efek nefroprotektif. Tujuan: Mengetahui efek dan sinergitas kombinasi MJH dan madu pada kadar malondialdehid (MDA) ginjal tikus yang diberi pajanan CP. Metode: Penelitian ini menggunakan 30 ekor tikus jantan yang dibagi menjadi 10 kelompok: Kontrol Normal (KN) (diet ad libitum), MJH1 (1 mL/kgBB MJH), MJH2 (2 mL/kgBB MJH), M1 (3,7 mL/kgBB M), M2 (7,4 mL/kgBB M), MJH1-M1 (1 mL/kgBB MJH dan 3,7 mL/kgBB M), MJH1-M2 (1 mL/kgBB MJH dan 7,4 mL/kgBB M), MJH2-M1 (2 mL/kgBB MJH dan 3,7 mL/kgBB M), MJH2-M2 (2 mL/kgBB MJH dan 7,4 mL/kgBB M), dan CP (diet ad libitum). MJH dan madu diberikan secara oral selama 21 hari. Pada hari ke-18, KN diberikan NaCl 0,9% 1 ml/kgBB sedangkan sisanya diberikan CP dosis tunggal 8 mg/kgBB secara intraperitoneal. Kadar MDA ginjal diukur menggunakan metode thiobarbituric acid reactive substances (TBARS). Didapatkan kadar MDA ginjal yang lebih rendah pada semua kelompok dosis tunggal dan kombinasi dibandingkan dengan kelompok CP (p=0,000). Semua kelompok dosis kombinasi memiliki indeks kombinasi <1. Kombinasi MJH dan madu memberikan efek protektif sinergis terhadap kerusakan oksidatif jaringan ginjal tikus akibat pajanan CP.

Kata kunci: Minyak jintan hitam, madu, indeks kombinasi, cisplatin, nefrotoksisitas


Abstract Cisplatin (CP) is a widely used chemotherapeutic agent that induces nephrotoxicity. Nigella sativa oil (NSO) and honey (H) have demonsrated nephroprotective effect. To investigate the effect and synergy of NSO and honey combination on kidney malondialdehyde (MDA) levels in CP-induced rats. Thirty male rats were divided into ten groups: Normal Control (NC), NSO1, NSO2, H1, H2, NSO1-H1, NSO1-H2, NSO2-H1, NSO2-H2, and CP-treated group which received ad libitum diet, 1 mL/kgbwt. NSO, 2 mL/ kgbwt. NSO, 3.7 mL/kgbwt. H, 7.4 mL/kgbwt. H, 1 mL/kgbwt. NSO and 3.7 mL/kgbwt. H, 1 mL/kgbwt. NSO and 7.4 mL/kgbwt. H, 2 mL/kgbwt. NSO and 3.7 mL/kgbwt. H, 2 mL/kgbwt. NSO and 7.4 mL/kgbwt. H, ad libitum diet, respectively, for 21 days. On the 18th day, NC group received 1 ml/kgbwt. NaCl 0.9% while the rest received 8 mg/kgbwt. single dose CP intraperitoneally. Kidney MDA levels was measured using thiobarbituric acid reactive substance (TBARS) method. Kidney MDA levels was significantly lower in all single and combination dose treatment groups compared to CP-treated group (p=0,000). Combination index of all combination dose treatment groups is <1. NSO and honey combination provided synergistic protective effect against CP-induced oxidative damage in kidney tissue in rats.

Keywords: Nigella sativa oil, honey, combination index, cisplatin, nephrotoxicity