Autentifikasi Obat Tradisional Tanpa Label Halal Yang Di Deteksi Melalui Real Time PCR

Abstract

abstrak
Dalam masyarakat mayoritas muslim, kehalalan suatu produk merupakan hal yang sangat penting. Pemerintah
Indonesia telah menerbitkan Undang Undang yang dapat memberikan jaminan kehalalan produk yang beredar di
Indonesia yang penduduknya mayoritas muslim. Obat tradisional merupakan salah satu produk yang beredar di
Indonesia juga harus memenuhi jaminan produk halal. Dalam peredarannya masih ada obat tradisional yang
belum tersertifikasi halal. Penelitian ini merupakan penelitian eksperimen yang bertujuan untuk mengetahui
autentifikasi yang belum tersertifikasi halal di wilayah Cilegon dengan menggunakan metode Real Time PCR.
Sebanyak 6 macam sampel obat tradisional terdiri dari 3 macam golongan Obat Tradisional Dalam Negeri (TR)
dan 3 macam golongan Obat Tradisonal Impor (TI) diuji menggunakan Real Time PCR). DNA gelatin masingmasing sampel diisolasi menggunakan kit komersial. Isolat DNA hasil isolasi diamplifikasikan pada Real Time
PCR sebanyak 30 siklus. Analisis data diambil dari hasil amplifikasi Real Time PCR. Dari hasil analisa melalui
metode Real Time PCR didapatkan hasil negatif dari enam sampel obat tradisional yang belum berlabel halal.
Kata kunci: Real Time PCR,halal, obat tradisional.
Abstract
In a Muslim majority society, the halalness of a product is very important. The Indonesian government has
issued a law that can guarantee the halalness of products circulating in Indonesia, which has a Muslim majority
population. Traditional medicine is one of the products circulating in Indonesia and must also meet the guarantee
of halal products. In its circulation there are still traditional medicines that have not been certified halal. This
research is an experimental study that aims to determine the authentication that has not been certified halal in the
Cilegon area by using the Real Time PCR method. A total of 6 samples of traditional medicines consisting of 3
types of Domestic Traditional Medicines (TR) and 3 types of Imported Traditional Medicines (TI) were tested
using Real Time PCR). The gelatin DNA of each sample was isolated using a commercial kit. Isolated DNA was
amplified in Real Time PCR for 30 cycles. Data analysis was taken from the results of Real Time PCR
amplification. From the results of the analysis through the Real Time PCR method, negative results were
obtained from six samples of traditional medicines that were not labeled halal.
Keywords: Real Time PCR, halal,traditional medicine