Kadar Sekresi IL-10 Hepar Mencit Galur BALB/c Setelah Pemberian Protein Pili 65,5 kDa Klebsiella pneumoniae

Abstract

ABSTRACT
Klebsiella pneumoniae belongs to the genus Klebsiella in the family Enterobacteriaceae, which is often found as a
nosocomial disease in hospitals. K. pneumoniae is easily resistant to antibiotics because it has the Extended-Spectrum
-Lactamase enzyme. The death rate from this bacterium reaches 28.3% and continues to increase. This study used
the organelle protein pili K. pneumoniae. This immune reaction can be measured by looking at the increased levels
of Interleukin-10 as a post-infection resolution protein, so the aim of the study was to find a relationship between
increased liver IL-10 levels and exposure to K. pneumoniae pili protein in mice. The variables in this study were IL10 levels in the liver of mice and pili protein concentrations of 65.5 kDa K. pneumoniae in each group. The correlation
analysis test of this study used the One-way Anova correlation test and continued with the Post Hoc test. This study
used 21 liver samples which were divided into 3 groups. The first group is the control group given PBS, the second
group is the treatment group with pili protein, and the third group is the adjuvant group. Liver samples were
processed using Sandwich ELISA. The results showed an increase in IL-10 levels in the treatment group given the
pili protein 65.5 kDa Klebsiella pneumoniae. The data also showed differences in the total mean of each group in IL10 levels after administration of K. pneumoniae pili protein (p=0.008). The treatment group had the highest mean
levels of IL-10 so there was an increased relationship between exposure to pili protein and IL-10 levels in mice based
on crude data. There was a significant increase in IL-10 levels in the treatment group compared to the adjuvant group
and there was an average increase in IL-10 levels in the treatment group compared to the control group, although
statistically the increase was not different and the relationship increased significantly.M.
Keywords: Klebsiella pneumoniae, Interleukin-10, pili protein 65,5 kDa.

ABSTRAK
Klebsiella pneumoniae termasuk genus Klebsiella dalam famili Enterobacteriaceae, sering ditemukan sebagai
penyakit nosokomial di rumah sakit. K. pneumoniae termasuk bakteri mudah resisten terhadap antibiotik
dikarenakan memiliki enzim Extended Spectrum β- Lactamase. Tingkat kematian akibat bakteri ini mencapai 28,3%
dan terus meningkat. Penelitian ini menggunakan organel protein pili K. pneumoniae. Reaksi imun ini dapat diukur
dengan melihat peningkatan kadar Interleukin-10 sebagai protein resolusi pasca infeksi sehingga tujuan penelitian
menemukan hubungan peningkatan kadar IL-10 hepar terhadap paparan protein pili K. pneumoniae pada mencit.
Variabel penelitian ini adalah kadar IL-10 pada hepar mencit dan kadar konsentrasi protein pili 65,5 kDa K.
pneumoniae pada setiap kelompok. Uji analisis korelasi penelitian ini menggunakan uji korelasi One-way Anova
dan dilanjutkan dengan uji Post Hoc. Penelitian ini menggunakan 21 sampel hepar yang dibagi dalam 3 kelompok.
Kelompok pertama adalah kelompok kontrol dengan pemberian PBS, kelompok kedua adalah kelompok perlakuan
dengan pemberian protein pili, dan kelompok ketiga adalah kelompok adjuvan. Sampel hepar diproses dengan
menggunakan Sandwich ELISA.Hasil penelitian menunjukkan peningkatan kadar IL-10 pada kelompok perlakuan
yang diberikan protein pili 65,5 kDa Klebsiella pneumoniae. Data juga menunjukan perbedaan mean total setiap
kelompok pada kadar IL-10 setelah pemberian protein pili K. pneumoniae (p=0,008). Kelompok perlakuan memiliki
mean kadar IL-10 tertinggi sehingga terdapat hubungan peningkatan antara paparan protein pili dengan kadar IL10 pada mencit secara data kasar. Peningkatan kadar IL-10 signifikan terdapat pada kelompok perlakuan dibanding
kelompok adjuvan dan terdapat peningkatan rata-rata kadar IL-10 pada kelompok perlakuan dibandingkan dengan
kelompok kontrol meskipun secara statistik peningkatan tidak berbeda dan hubungan peningkatan secara signifikan.
Kata kunci: Klebsiella pneumoniae, Interleukin-10, protein pili 65,5 kDa.