Isolasi dan Skrining Aktivitas Antibakteri Fungi Tanah Muara Desa Katialada Gorontalo Lokasi Satu terhadap Staphylococcus aureus

Wilda Nur Rohmatillah; Muhammad Zainul Arifin; Saeful Akhmad Tauladani; Gani Asri Muharam; Asia Asia; Dwi Koko Pratoko; Ari Satia Nugraha

doi:10.22435/jki.v0i0.5978

Wilda Nur Rohmatillah Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Negeri Jember, Jember, Indonesia
Muhammad Zainul Arifin Politeknik Kelautan & Perikanan Bitung, Kota Bitung, Indonesia
Saeful Akhmad Tauladani Politeknik Kelautan & Perikanan Bitung, Kota Bitung, Indonesia
Gani Asri Muharam Balai Pendidikan dan Pelatihan Perikanan Aertembaga, Kota Bitung, Indonesia
Asia Asia Politeknik Kelautan dan Perikanan Bone, Kabupaten Bone, Indonesia
Dwi Koko Pratoko Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Negeri Jember, Jember, Indonesia
Ari Satia Nugraha Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Negeri Jember, Jember, Indonesia

DOI: https://doi.org/10.22435/jki.v0i0.5978

Keywords: Antibakteri, Staphylococcus aureus, Isolasi fungi tanah

Abstract

The emergence of antibiotic resistance becomes a barrier in controlling infectious diseases, so it is necessary to find new alternative antibiotics. This study aimed to explore the potential of estuarine soil fungals secondary metabolites isolated from Katialada Village Gorontalo Location One and test their antibacterial activity against Staphylococcus aureus. The antibacterial parameter observed was the percent inhibition of ethyl acetate extracts against Staphylococcus aureus. The estuarine soil samples were cultured in PDA media producing five single fungal isolates differentiated based on color morphology, texture, and shape coded IS2-BTG-4.1.1, IS2-BTG-4.1.2, IS1-BTG-4.2, IS2-BTG4.3.1, and IS2-BTG-4.3.2. Those five samples were fermented using PDB media and the fermented products were extracted with ethyl acetate. The extracts were screened for its compound using a TLC plate and antibacterial testing with a single concentration of 100 g/mL in the microdilution method. The screening results showed that the five ethyl acetate extracts contained terpenoid compounds and code IS2-BTG-4.3.2 also had alkaloids. Based on the antibacterial test results, the five ethyl acetate extracts had growth inhibitory activity on the Staphylococcus aureus, with the percentage showed by IS2-BTG-4.1.1 50,2% ± 14,8%, IS2-BTG-4.1.2 35,6% ± 12,9%, IS1-BTG-4.2 13,0% ± 7,3%, IS2-BTG-4.3.1 13,6% ± 6,0%, and IS2-BTG-4.3.2 by 12,4% ± 9,2%.

Author Biographies

Wilda Nur Rohmatillah, Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Negeri Jember, Jember, Indonesia

Drug Utilitation and Discovery Research Groups, Fakultas Farmasi

Saeful Akhmad Tauladani, Politeknik Kelautan & Perikanan Bitung, Kota Bitung, Indonesia

Politeknik Kelautan & Perikanan Bitung; Australian National Centre for Ocean Resources and Security (ANCORS)-UOW

Dwi Koko Pratoko, Program Studi Sarjana Farmasi, Fakultas Farmasi, Universitas Negeri Jember, Jember, Indonesia

Drug Utilisation and Discovery Research Groups

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