Determination of HPLC Chromatogram Profile of Katuk (Breynia androgyna (L.) Chakrab.  & N.P.Balakr) Plants from Ristoja's Results using  Chemometric Analysis

Nanang Yunarto; Sukmayati Alegantina; Ani Isnawati

doi:10.22435/jki.v0i0.6040

Nanang Yunarto Center for Research and Development, Biomedical and Basic Health Technology, National Institute of Health Research and Development, Jakarta, Indonesia
Sukmayati Alegantina National Research and Innovation Agency, Jakarta, Indonesia
Ani Isnawati National Research and Innovation Agency, Jakarta, Indonesia

DOI: https://doi.org/10.22435/jki.v0i0.6040

Keywords: Breynia androgyna, Chromatogram profile, HPLC, Chemometric

Abstract

The katuk plant was known as Sauropus androgynous (L.) Merr, but the name is changed to Breynia androgyna (L.) Chakrab. & N.P. Balakr since the publication of Chakrab's & N.P. Balakr in the 2012 Journal of Plant Taxonomists. The content of chemical compounds and secondary metabolites of katuk leaf are strongly influenced by different habitat or location. Therefore, the Center for Research and Development of Medicinal Plants and Traditional Medicines, National Institute of Health Research and Development (NIHRD) has conducted Research on medicinal plants and herbs (RISTOJA) results on 58 samples of katuk plants originating from 7 provinces and used by 13 ethnic groups in Indonesia. This study aims to obtain plant quality based on the description of the High-Performance Liquid Chromatography (HPLC) chromatogram profile, in order to obtain the suitability of the efficacy of medicinal plants with their benefits for the community in an area. Test using HPLC with a gradient mobile phase, mixture of acetonitrile and methanol for 60 minutes. The results of the chromatogram were analyzed chemometrically by using Principal Component Analysis (PCA) data interpretation. PCA results showed that from HPLC chromatograms at 254 nm and 366 nm, each gave 3 different clusters, namely Clusters A, B and C where each cluster has the same chromatogram profile of katuk plants. Cluster A which was identified at a wavelength of 254 nm was the most used cluster by 7 ethnic groups with a total of 28 Katuk samples. Similarly, the chromatogram at a wavelength of 366 nm with the largest cluster is cluster A which is used by 11 ethnic groups with a total of 45 samples of katuk plants. where each cluster had the same chromatogram profile of katuk Plants.

References

Bunawan H, Bunawan SN, Baharum SN, Noor NM. Sauropus androgynus (L.) Merr. induced bronchiolitis obliterans: from botanical studies to toxicology. Evidence-based Complementary Alternative Medicine. 2015;2015.

Yustendi D, Mardhiah A. Pemberian ekstrak daun katuk (Sauropus androgynus L. Merr) dalam air minum kambing peranakan ettawa untuk meningkatkan produksi dan kualitas susu. Jurnal Agripet. 2018;18(2):90–4.

Irwansyah AE. Efek komponen bioaktif ekstrak daun torbangun (Coleus amboinicus L) terhadap kadar dan ekspresi gen-gen reseptor hormon laktogenik pada tikus laktasi. Disertasi. 2018;

Santoso U, Fenita Y, Sulistyowati E. Effect of Sauropus androgynus leaf extract, fish oil and vitamin E on performance, egg quality and composition in laying hens. Jurnal Agripet. 2021;21(2):105–12.

Chakrabarty T, Balakrishnan NP. Nineteen new combinations and a new name in Breynia J.R. Forst. & G. Forst. (Phyllanthaceae) from Indian subcontinent. Bangladesh Journal Plant Taxonnomy. 2012;19(2):119–22.

Ngadiarti I, Agestika L, Srimiati M, Fayasari A. Sauropus androgynus, Papaya Leaves, and Mung Beans as mixed galactagogue drink for urban postpartum mothers. Jurnal Gizi dan Pangan. 2021;16(2):101–8.

Awaludin, Kartina, Maulianawati D, Manalu W, Andriyanto, Septiana R, et al. Phytochemical screening and toxicity of ethanol extract of Sauropus androgynus. Biodiversitas. 2020;21(7):2966–70.

Bernard D, Kwabena A, Osei O, Daniel G, Elom S, Sandra A. The effect of different drying methods on the phytochemicals and radical scavenging activity of Ceylon Cinnamon (Cinnamomm zeylanicum) plant parts. European Journal of Medicinal Plants. 2014;4(11):1324–35.

Rababah T, Aludatt M, Alhamad M, Noor. Effects of drying process on total phenolics, antioxidant activity and flavonoid contents of common mediterranean herbs. International Journal of Agricultur and Biological Engineering. 2015;8(2):145–50.

Warditiani NK, Indrani A, Sari N, Swasti IAS, .Pengaruh pemberian fraksi terpenoid daun katuk (Sauropus Androgynus (L.) Merr) terhadap profil lipid tikus putih (Rattus Novergicus, L.) jantan galur wistar yang diinduksi pakan kaya lemak. Jurnal Farmasi Udayana [Internet]. 2015;4(2):66–71. Available from: https://media.neliti.com/media/publications/279791-pengaruh-pemberian-fraksi-terpenoid-daun-890eebe3.pdf

S, Rahmanisa TA. Efektivitas ekstraksi alkaloid dan sterol daun Katuk (Sauropus androgynus) terhadap produksi ASI. Majority [Internet]. 2016;5(1):117–21. Available from: https://juke.kedokteran.unila.ac.id/index.php/majority/article/viewFile/991/719

Nuryani. Kenali mutu daun kumis kucing ( Orthosiphon stamineus ) meggunakan pengolahan citra dan teknik pengenalan pola secara kemometrik. Skripsi, Fakultas Matematika dan Ilmu Pengetahuan Alam IPB. 2015;

Susanti S. Optimasi metode High Performance Liquid Chromatography pada penetapan kadar piperin Piper ningrum Linn. Skripsi. 2019;1–9.

USP. Chromatography USP 621 National Formulary. Pharmacopeial Convention Inc; 2009.

Nurnasari E, Djumali. Pengaruh kondisi ketinggian tempat tumbuh terhadap produksi dan mutu tembakau Temanggung. Buletin Tanaman Tembakau, Serat & Minyak Industri. 2010;2(2):45–59.

Shafirany MZ, Susiilawati Y, Musfiroh I. Aplikasi kemometrik dalam penentuan mutu tumbuhan obat. Pharmauho Jurnal Farmasi Sains, dan Kesehatan. 2019;4(2);6-14..

Ambrożej KK, Karpinska J. The application of spectroscopic techniques in combination with chemometrics for detection adulteration of some herbs and spices. Microchemical Journal. 2020;153(104278):1–9.

Wang P, Yu Z. Species authentication and geographical origin discrimination of herbal medicines by near infrared spectroscopy: A review. Journal Pharmacy Analysis [Internet]. 2015;5(5):277–84. Available from: http://dx.doi.org/10.1016/j.jpha.2015.04.001

Alegantina S, Setyorini HA, Oktoberia IS, Winarsih AN. Profil Physalis minima L. dari berbagai etnis di 9 (sembilan) Provinsi Indonesia secara HPLC dan kemometrik. Media Penelitian dan Pengembangan Kesehatan. 2021;31(1):17–26.