Efektivitas Gel Ekstrak Etanol 70% Daun Anggrek Oncidium (Oncidium Aliceara alice) terhadap Penyembuhan Luka Kulit Dorsum Tikus Sprague Dawley

Michele Liony Maria Onibala; Maria Ariska Happy Suntadi; Jovitta Theachrysilla Wiadji; Yustina Wanda Dwi Oktaviani; Yohanes Candra Gunawan; Stefani Santi Widhiastuti

doi:10.22435/jki.v13i1.6211

Michele Liony Maria Onibala Program Studi Biologi, Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Yogyakarta, Indonesia
Maria Ariska Happy Suntadi Program Studi Biologi, Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Yogyakarta, Indonesia
Jovitta Theachrysilla Wiadji Program Studi Biologi, Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Yogyakarta, Indonesia
Yustina Wanda Dwi Oktaviani Program Studi Biologi, Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Yogyakarta, Indonesia
Yohanes Candra Gunawan Program Studi Biologi, Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Yogyakarta, Indonesia
Stefani Santi Widhiastuti Program Studi Biologi, Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Yogyakarta, Indonesia

DOI: https://doi.org/10.22435/jki.v13i1.6211

Keywords: Oncidium Aliceara alice, Wound healing, Gel, Cathecins

Abstract

Wounds are tissue damage that is generally caused by physical contact. Wound healing is a complex mechanism involving biochemical and biocellular processes. Various therapies can be done to speed up the wound healing process, one of which is treatment using natural ingredients. One of the plants that have the potential to heal wounds is Oncidium Aliceara alice. The purpose of this study was to determine the phytochemical content of Oncidium Aliceara alice and its activity in wound healing when it is formulated in a gel form. This research is experimental research with extraction stages, phytochemical assay, gel formulations, quality test, and wound healing activity tests in animals. The gel formulation was made with various concentrations of Oncidium Aliceara alice extract 2.5%, 5% and 10%. Male Sprague Dawley rats were given an incision wound on the dorsum of the longitudinal axis along 3 cm. Wound care by administering topical Bioplacenton as a positive control, base gel as a negative control, and gel extract concentration variants according to each treatment group. Based on the research, the results of qualitative phytochemical tests showed that the 70% ethanol extract of Oncidium Aliceara alice contains flavonoids (catechins), tannins, steroids, and saponins. The best results were seen on the 7th day of observation for the 10% gel extract treatment group with more perfect signs of wound closure than the bioplacenton treatment.

References

Kartika RW, Bedah B, Paru J, Luka AP. Perawatan Luka Kronis dengan Modern Dressing. CDK Journal. 2015;42(7):546–50.

Sakum, Hartoyo M, Utomo TA. Pengaruh Pengelolaan Teknik Aseptik Pada Penanganan Luka Terbuka Stadium II-III Terhadap Kejadian Infeksi Di Rumah Sakit Umum Daerah Sunan Kalijaga Demak. E-journal Poltekkes. 2013;9(1) 474–81.

Purnama H, Sriwidodo, Ratnawulan S. Review Sistematik: Proses Penyembuhan dan Perawatan Luka. Farmaka. 2017;15(2):251–6. https://doi.org/10.24198/jf.v15i2.13366.g6184.

Ganesan P, Schmiedge J, Manchaiah V, Swapna S, Dhandayutham S, Kothandaraman PP. Ototoxicity: A challenge in diagnosis and treatment. J Audiol Otol. 2018;22(2):59–68. https://doi.org/10.7874/jao.2017.0036.

Chawla S, Chaudhary T, Aggarwal S, Maiti GD, Jaiswal K, Yadav J. Ophthalmic considerations in pregnancy. Med J Armed Forces India. 2013;63(3):278–84. https://doi.org/10.1016/j.mjafi.2013.03.0.

Suhendar U, Utami NF, Sutanto D, Nurdayanty SM. Pengaruh Berbagai Metode Ekstraksi pada Penentuan Kadar Flavonoid Ekstrak Etanol Daun Iler (Plectranthus scutellarioides). Fitofarmaka J Ilm Farm. 2020; 10(1):76-83.

Nurzaman F, Djajadisastra J, Elya B. Identifikasi Kandungan Saponin dalam Ekstrak Kamboja Merah (Plumeria rubra L.) dan Daya Surfaktan dalam Sediaan Kosmetik. J Kefarmasian Indones. 2018;8(2):85–93.

Illing I, Safitri W, Erfiana. Uji Fitokimia Ekstrak Buah Degen Ilmiati Illing, Wulan Safitri dan Erfiana. J Din. 2017;8(1):66–84.

Akcaya JB, Hidayat B, Yusro F, Mariani Y, Kehutanan F, Tanjungpura U, et al. Kemampuan Ekstrak Kulit Kayu Dua Spesies Macaranga Dalam Menghambat Pertumbuhan Bakteri Enterococcus Faecalis the Ability of Two Species of Macaranga Wood Bark Extracts To Inhibit the Growth of Bacteria Enterococcus Faecalis. 2019;5(2):95–109.

Indarto I. Uji Kualitatif Dan Kuantitatif Golongan Senyawa Organik Dari Kulit Dan Kayu Batang Tumbuhan Artocarpus Dadah Miq. J Ilm Pendidik Fis Al-Biruni. 2015;4(1):75–84.

Prayoga, dkk. Antioksidan Ekstrak Kasar Daun Pepe ( Gymnema Reticulatum Br .) Pada Berbagai Jenis Pelarut. J Ilmu dan Teknol Pangan. 2019;8(2):111–21.

Nugrahani R, Andayani Y, Hakim A. Skrining Fitokimia dari Ekstrak Buah Buncis (Phaseolus vulgaris L) dalam Sediaan Serbuk. J Penelit Pendidik IPA. 2016;2(1):96-103.

Ligor M, Kornyšova O, Maruška A, Buszewski B. Determination of flavonoids in tea and Rooibos extracts by TLC and HPLC. J Planar Chromatogr - Mod TLC. 2008;21(5):355–60.

Irianto IDK, Purwanto P, Mardan MT. Aktivitas Antibakteri dan Uji Sifat Fisik Sediaan Gel Dekokta Sirih Hijau (Piper betle L.) Sebagai Alternatif Pengobatan Mastitis Sapi. Maj Farm. 2020;16(2):202.

Petersen H, Tavakoli F, Kruber S, Münscher A, Gliese A, Hansen NO, et al. Comparative study of wound healing in rat skin following incision with a novel picosecond infrared laser (PIRL) and different surgical modalities. Lasers Surg Med. 2016;48(4):385–91.

Kim E, Hwang K, Lee J, Han SY, Kim EM, Park J, et al. Skin protective effect of epigallocatechin gallate. Int J Mol Sci. 2018;19(1):1–14.

Han SY, Kim E, Hwang K, Ratan ZA, Hwang H, Kim EM, et al. Cytoprotective effect of epigallocatechin gallate (EGCG)-5′-O-α-glucopyranoside, a novel EGCG derivative. Int J Mol Sci. 2018;19(5).

De Gaspi FODG, Foglio MA, De Carvalho JE, Santos GMT, Testa M, Passarini JR, et al. Effects of the topical application of hydroalcoholic leaf extract of Oncidium flexuosum Sims. (Orchidaceae) and microcurrent on the healing of wounds surgically induced in Wistar rats. Evidence-based Complement Altern Med. 2011;2011.

Charissa, M, Djajadisastra J, Elya B. Uji aktivitas antioksidan dan penghambatan tirosinase serta uji manfaat gel ekstrak kulit batang taya (Nauclea subdita) terhadap kulit. Jurnal Kefarmasian Indonesia. 2016;6(2):98-107.

Indriaty S. Formulasi Dan Uji Stabilitas Gel Antiaging Dari Kombinasi Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus Polyrhizus) Dan Lendir Bekicot (Achatina Fulica) Dengan Variasi Gelling Agent Carbomer 940 1%, 1,25%, 1,5%Dan 1,75%. J Pharmacopolium. 2019;2(2):104–11.

Prasongko ET, Lailiyah M, Muzayyidin W. Formulasi Dan Uji Efektivitas Gel Ekstrak Daun Kedondong ( Spondias dulcis F .) Terhadap Luka Bakar Pada Tikus Wastar ( Rattus novergicus ). J Wiyata S1 Farm Fak Farm ,Institut Ilmu Kesehat Bhakti, Kesehat Bhakti Wiyata. 2020;7(10):27–36.

Chen Y, Tian L, Yang F, Tong W, Jia R, Zou Y, Yin L, Li L, He C, Liang X, Ye G, Lv C, Song X, Yin Z. Tannic acid accelerates cutaneous wound healing in rats via activation of the erk ½ signaling pathways. advances in wound care. 2019; 8 (7):341-354.

Su X, Liu X, Wang S, Li B, Pan, T, Liu D, Wang F, Diao Y, Li K. Wound-healing promoting effect of total tannins from Entada phaseoloides (L.) Merr. in rats. Burns. 2017;43(4):830-838.

Carvalho MTB, Araujo-Filho HG, Barreto AS, Quintans-Junior LJ, Quintans JSS, Barreto RSS. Wound healing properties of flavonoids: a systematic review of highlighting the mechanisms of action. phytomedicine. 2021;90(1):1-15.

Aslam MS, Ahmad MS, Riaz H, Raza SA, Hussain S, Qureshi QS, Maria P, Hamzah Z, Javed O. Phytochemicals: Source of Antioxidants and Role in Disease Prevention. London:IntechOpen; 2018

Razika L, Chaoche TA, Nadjiba C, Narimen, B. Antioxidant and wound healing potential of saponins extracted from the leaves of Algerian Urtica dioica L. Pakistan J of Pharmaceutical Sciences. 2017;30(3):1023-29.

Wang AS, Armstrong EJ, Armstrong AW. Corticosteroids and wound healing: clinical considerations in the perioperative period. The American Journal of Surgery. 2013;206(3):410-7.

Kuswahyuning R, Lesmana I. Formulation and evaluation of mangosteen (Garcinia mangostana L.) fruit pericarp extract gel. Jurnal Kefarmasian Indonesia. 2021;11(2):90-7.

Wicke C, Halliday B, Allen D, Roche NS, Scheuenstuhl H, Spencer MM, Roberts AB, Hunt TK. Effects of steroids and retinoids on wound healing. The Archives of Surgery. 2000;135(11):1265-70.