Validasi Metode Analisis Penetapan Kadar Protein Ekstrak Ikan Gabus dengan Metode Lowry dan Bromocresol Green

Andi Suhendi; Abdul Rohman; Safira Cahyaningrum

doi:10.22435/jki.v13i1.6219

Andi Suhendi Bagian Kimia Farmasi, Fakultas Farmasi Universitas Muhammadiyah Surakarta, Surakarta, Indonesia
Abdul Rohman Departemen Kimia Farmasi, Fakultas Farmasi Universitas Gadjah Mada, Yogyakarta, Indonesia
Safira Cahyaningrum Bagian Kimia Farmasi, Fakultas Farmasi Universitas Muhammadiyah Surakarta, Surakarta, Indonesia

DOI: https://doi.org/10.22435/jki.v13i1.6219

Keywords: Snakehead fish, Lowry, Bromocresol-green, Spectrophotometer, Analytical method validation

Abstract

Snakehead fish is one of the biological resources with high economic value because it contains high albumin levels. Analytical methods for the determination of protein content in snakehead fish are available not been validated. This study aims to obtain a comparison of the accuracy, repeatability, intermediate precision, and linearity between the Lowry and Bromocresol-green methods in determining albumin levels from snakehead fish extracts. This study was performed with the SHIMADZU UV-1280 spectrophotometer at a wavelength of 778 nm for the lowry method and 638 nm for the bromocresol-green method. The results of the study show that all parameters meet the requirements for accepting validation. The results were tested statistically with the independent t-test, the p-value was 0.172 (p> 0.05) the accuracy between the Lowry and Bromocresol-green methods did not have a significant difference, that the Lowry and Bromocresol green methods had the same ability to determine accuracy parameters and precision parameters, the p-value is 0.000 (p <0.05) the precision between the Lowry and bromocresol-green methods has a significant difference, that the bromocresol-green method is better than the Lowry method.

References

Harianti. Ikan gabus (Channa striata) dan berbagai manfaat albumin yang terkandung di dalamnya. Jurnal Balik Diwa. 2011;2(2):18–25.

Ab Wahab SZ, Abdul Kadir A, Nik Hussain NH, Omar J, Yunus R, Baie S, et al. The effect of Channa striatus (haruan) extract on pain and wound healing of post-lower segment caesarean section women. Evid Based Complement Alternat Med. 2015; 1-6. doi: 10.1155/2015/849647

Abu Bakar MR, Abdul Kadir A, Abdul Wahab SZ, Abdul Karim AH, Nik Hussain NH, Mohd Noor N, et al. Randomized controlled trial on the effect of Channa striatus extract on measurement of the uterus, pulsatility index, resistive index of uterine artery and superficial skin wound artery in post lower segment caesarean section women. PLoS One. 2015;10(7):1-11. doi: 10.1371/journal.pone.0133514

Fajri UN, Hadisaputro S, Soejoenoes A. The effect of snake fish extract (Channa striata) on post cesarean section wound status in postpartum anemia mothers. Indonesian Journal of Medicine. 2018;3(2):84–8.

Asfar M, Tawali AB, Mahendradatta M. Potensi ikan gabus (Channa striata) sebagai sumber makanan kesehatan. In: Prosiding Seminar Nasional Teknologi Industri II. Makassar; 2014. p. 150–4.

Asikin AN, Kusumaningrum I. Edible portion dan kandungan kimia ikan gabus (Channa striata) hasil budidaya kolam di Kabupaten Kutai Kartanegara, Kalimantan Timur. Ziraa’ah Majalah Ilmiah Pertanian. 2017;42(3):158–63.

Fitriyani E, Meidy Deviarni I. Pemanfaatan ekstrak albumin ikan gabus (Channa striata) sebagai bahan dasar cream penyembuhan luka. Vokasi. 2016; 9(3):166-74

Chasanah E, Nurilmala M, Purnamasari AR, Fithriani D. Komposisi kimia, kadar albumin dan bioaktivitas ekstrak protein ikan gabus (Channa striata) alam dan hasil budidaya. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan. 2015;10(2):123–32.

Suhendi A, Pawarti H, Rohman A, Wahyono D, Nurrochmad A. Snakehead fish extract (channa striata): a review of pharmacological activity. EurAsian Journal of Biosciences. 2020;14(2): 4527-33.

Sari FA, Handayani S, Nurhaini R. Penetapan kadar albumin dalam ikan gabus (Channa striata) kukus dengan metode spektrofotometri visibel. CERATA Jurnal Ilmu Farmasi. 2016;6(1):8-17

Putri AAB, Yuliet Y, Jamaluddin J. Analisis kadar albumin ikan sidat (Anguilla marmorata dan Anguilla bicolor) dan uji aktivitas penyembuhan luka terbuka pada kelinci (Oryctolagus cuniculus). 2016;2(2):90–5.

Alamsjah MA, Kusumaningrum GA, Masithah ED. Uji kadar albumin dan pertumbuhan ikan gabus (Channa striata) dengan kadar protein pakan komersial yang berbeda. JIPK. 2014;6(1):25–30.

Martina V, Vojtech K. A comparison of biuret, lowry and bradford methods for measuring the egg’s proteins. In Faculty of Agronomy, Cz; 2015. 394–8.

Moosavi SM, Ghassabian S. Linearity of calibration curves for analytical methods: a review of criteria for assessment of method reliability. Calibration and validation of analytical methods - a sampling of current approaches. 2018. doi: 10.5772/intechopen.72932

Shen CH. Chapter 8 - Quantification and analysis of proteins. In: shen ch, editor. Diagnostic molecular biology. Academic Press; 2019. p. 187–214.

Taghreed A. Measurementof serum albuminby three different methods. kerbala journal of pharmaceutical sciences. 2013;(4):111-18.

Yunarto N, Reswandaru UN, Sulistyowati I, Prameswari IO, Pinanditi QL, & Patadungan TM. Validation of spectrophotometry method for determination of (+)-catechin in ethyl acetate fraction of gambir extract (Uncaria gambir Roxb.). Jurnal Tumbuhan Obat Indonesia. 2021;14(2):127-36.

Harmono HD. Validasi metode analisis logam merkuri (hg) terlarutn pada air permukaan dengan automatic mercury analyzer. Indonesian Journal of Laboratory. 2020 Aug 19;2(3):11–6.

Peris-Vicente J, Esteve-Romero J, Carda-Broch S. Validation of analytical methods based on chromatographic techniques: an overview. In: Analytical Separation Science. John Wiley & Sons, Ltd; 2015. p. 1757–808.