FTA CARDS SEBAGAI TEMPAT PENYIMPANAN SPESIMEN YANG OPTIMAL DAN SESUAI DENGAN ASPEK BIOSAFETY

  • Risqa Novita
Keywords: FTA cards, biosafety, penyimpanan spesimen

Abstract

Pengambilan spesimen di lapangan, terutama di daerah terpencil memiliki risiko karena memerlukan rantai dingin agar spesimen tetap dalam kondisi segar. Namun fasilitas laboratorium di daerah terpencil masih banyak yang kurang lengkap seperti tidak terdapat freezer untuk menyimpanan spesimen, dan transportasi ke daerah terpencil memerlukan waktu lama. Untuk mengatasi hal itu, diperlukan suatu teknik penyimpanan yang tidak membutuhkan freezer dan sesuai dengan aspek biosafety karena spesimen mengandung partikel infeksius. FTA cards merupakan teknologi terbaru untuk penyimpanan dan penanganan spesimen yang sesuai drengan aspek biosafety namun hingga saat ini pemakaian FTA cards masih belum banyak digunakan. Tujuan dari penulisan ini untuk mengkaji FTA cards dari berbagai aspek agar menjadi rujukan dalam memilih tehnik penyimpanan spesimen dibandingkan dengan metode konvensional sehingga para pembaca dapat mengetahui kegunaan FTA cards secara luas yang dapat dipakai dalam surveilans penyakit menular dan penyakit tidak menular. Tulisan ini merupakan kajian dari literatur-literatur yang ada di Google scholar dan Pubmed, dengan pencarian menggunakan kata kunci FTA cards, biosafety dan penyimpanan spesimen. Berdasarkan hasil dari penelusuran literatur, didapatkan hasil bahwa FTA cards dapat digunakan sebagai media penyimpanan spesimen. Spesimen yang dapat diteteskan ke atas permukaan FTA cards adalah darah, serum, cairan tubuh, feses dan organ tubuh hewan lainnya seperti nyamuk. FTA cards dapat direkomendasikan sebagai tehnik penyimpanan spesimen pada surveilans penyakit menular dan penyakit tidak menular namun memiliki harga yang lebih mahal dibandingkan dengan penyimpanan konvensional

 

Collecting of infectious specimens in the field, particularly in the remote areas is risky, because they need cold chain for keep it fresh. Unfortunately, the facilities of laboratory in remote areas are not complete. There are no freezer to storage the specimens and the transportation to the remote areas limited. To solve the problem, there should be a storage technique which do not need the freezer and related to biosafety aspect, because the specimen are infectious. FTA cards is the latest technology for storage and handling specimens which related to biosafety aspect, but until now FTA cards is not widely used. The purpose of this writing is to review FTA cards from various aspects and compared it with the conventional storage, so the readers would know about the widely usage of FTA cards, especially in surveillance of infectious diseases. The methods of this writing is study from the literatures on google scholar and pubmed , by using keywords FTA cards, biosafety and storage specimen. Based on the results of tracing the literatures, obtained the results that FTA cards could be used as a storage specimens. Blood, serum, body fluids, faeces and body organs from animals as the mosquito can drizzling upon the surface of FTA cards. FTA cards can be recommended as the best storage technique of the specimen on surveillance of infectious disease and non infectious diseases but FTA cards are more expensive compared with the conventional storage

References

1. Arredondo M, Garrido C, Parkin N, Zahonero N, Bertagnolio S, et al (2012) Comparison of HIV-1 RNA measurements obtained by using plasma and dried blood spots in the automated Abbott Real-Time viral load assay. J Clin Microbiol 50(3):569–72.
2. Marconi A, Balestrieri M, Comastri G, Pulvirenti FR, Gennari W, et al (2009) Evaluation of the Abbott Real-Time HIV-1 quantitative assay with dried blood spot specimens. Clin Microbiol Infect 15(1):93–7.
3. Vidya M, Saravanan S, Rifkin S, Solomon SS, Waldrop G, et al (2012) Dried blood spots versus plasma for the quantitation of HIV-1 RNA using a Real-Time PCR, m2000rt assay. J Virol Methods 181(2):177–81. Epub 2012/03/10.
4. Smith P.W., et al. Systematic Review of the Use of Dried Blood Spots for Monitoring HIV Viral Load and for Early Infant Diagnosis. PLOS ONE. 2014:9(3);1-8.
5. Song et al., Preservation Methods Differ in Fecal Microbiome Stability, Affecting
Suitability for Field Studies. Applied and environmental science. 2016.1(3); e00021-16
6. Dominianni C, Wu J, Hayes RB, Ahn J. Comparison of methods for fecal microbiome biospecimen collection. BMC Microbiol.2014:14;103.
7. Chin J., Manual Pemberantasan Penyakit Menular, Ed ke 17, I Nyoman Kadun : Penerjemah: Jakarta UI, Terjemahan dari Control of Communicable disease, 2007
8. Peluso A.L., Use of FTA Cards for the Storage of Breast Carcinoma Nucleic Acid on Fine-Needle Aspiration Samples. Cancer cytopathology. 2015. 585-592.
9. Saieg MA, Geddie WR, Boerner SL, et al. The use of FTA cards for preserving unfixed cytological material for high-throughput molecular analysis. Cancer (Cancer Cytopathol). 2012;120:206-214.
10. Wolfgramm Ede V, de Carvalho FM, Aguiar VR, et al. Simplified buccal DNA extraction with FTA Elute Cards. Forensic Sci Int Genet. 2009;3:125-127.
11. Lenselink CH, de Bie RP, van Hamont D, et al. Detection and genotyping of human papillomavirus in self-obtained cervicovaginal samples by using the FTA cartridge: new possibilities for cervical cancer screening. J Clin Microbiol. 2009;47:2564-2570.
12. Hansen TV, Simonsen MK, Nielsen FC, Hundrup YA. Collection of blood, saliva, and buccal cell samples in a pilot study on the Danish nurse cohort: comparison of the response rate and quality of genomic DNA. Cancer Epidemiol Biomarkers Prev. 2007;16:2072-2076.
13. Smith LM, Burgoyne LA. Collecting, archiving and processing and DNA from wildlife samples using FTA databasing paper. BMC Ecol. 2004;4:4.
14. Lenselink CH, de Bie RP, van Hamont D, et al. Detection and genotyping of human papillomavirus in self-obtained cervicovaginal samples by using the FTA cartridge: new possibilities for cervical cancer screening. J Clin Microbiol. 2009;47:2564-2570.
15. Wolfgramm Ede V, de Carvalho FM, Aguiar VR, et al. Simplified buccal DNA extraction with FTA Elute Cards. Forensic Sci Int Genet. 2009;3:125-127.
16. Gustavsson I, Lindell M, Wilander E, Strand A, Gyllensten U. Use of FTA card for dry collection, transportation and storage of cervical cell specimen to detect high-risk HPV. J Clin Virol. 2009;46:112-116.
17. Milne E, van Bockxmeer FM, Robertson L, et al. Buccal DNA collection: comparison of buccal swabs with FTA cards. Cancer Epidemiol Biomarkers Prev. 2006;15:816-819.
18. Rottinghaus EK, Ugbena R, Diallo K, Bassey O, Azeez A, et al. Dried blood spot specimens are a suitable alternative sample type for HIV-1 viral load measurement and drug resistance genotyping in patients receiving first-line
antiretroviral therapy. Clin Infect Dis. 2012:54(8);1187–95.
19. Ahmed Heba A.,Ewan T MacLeod, Geoff Hide, Susan C Welburn and Kim Picozzi. The best practice for preparation of samples from FTA®cards for diagnosis of blood borne infections using African trypanosomes as a model system.Parasites & Vectors.2011:4(68);1-7.
20. Owor BE, Shepherd DN, Taylor NJ, Edema R, Monjane AL, Thomson JA, Martin DP and Varsani. A: Successful application of FTA® Classic Card technology and use of bacteriophage [phi]29 DNA polymerase for largescale field sampling and cloning of complete maize streak virus genomes. J Virol Methods.2007:140;100-105.
21. Catarino Rosa, et al., Randomized Comparison of Two Vaginal Self-Sampling Methods for Human Papillomavirus Detection: Dry Swab versus FTA Cartridge. PLOS One. 2015;1-13.
22. Guan Y, Gravitt PE, Howard R, Eby YJ, Wang S, Li B, et al. Agreement for HPV genotyping detection between self-collected specimens on a FTA cartridge and clinician-collected specimens. J Virol Methods. 2013; 189(1):167–71.
23. De Bie RP, Schmeink CE, Bakkers JM, Snijders PJ, Quint WG, Massuger LF, et al. The indicating FTA elute cartridge: a solid sample carrier to detect high-risk HPV and high-grade cervical lesions. J Mol Diagn. 2011; 13(4):371–6.
24. Johanson, H.C., V. Hyland, C. Wicking, and R.A. Sturm. DNA elution from buccal cells stored on Whatman FTA Classic Cards using a modified methanol fixation method. Biotechniques.2009:46;309-311.
25. Kampmann Marie L., Anders Buchard, Claus Børsting, and Niels Morling. High throughput sequencing of forensic genetic samples using punches of FTA cards with buccal swabs. BioTechniques. 2016:61(3);149-151
Published
2017-11-24
How to Cite
1.
Novita R. FTA CARDS SEBAGAI TEMPAT PENYIMPANAN SPESIMEN YANG OPTIMAL DAN SESUAI DENGAN ASPEK BIOSAFETY. sel [Internet]. 24Nov.2017 [cited 14Jul.2020];4(2):81-0. Available from: http://ejournal2.litbang.kemkes.go.id/index.php/sel/article/view/1469