AKTIVITAS ANTIBAKTERI MINYAK SERAI DAPUR dan MINYAK ADAS pada Staphylococcus aureus di RUANG INAP RUMAH SAKIT

Khoirun Nisyak; Susi Hartiningsih

doi:10.22435/jtoi.v13i2.2227

Khoirun Nisyak STIKES Rumah Sakit Anwar Medika
Susi Hartiningsih Stikes Rumah Sakit Anwar Medika

DOI: https://doi.org/10.22435/jtoi.v13i2.2227

Keywords: antibakteri, minyak serai dapur, minyak adas, S.aureus, rumah sakit

Abstract

ABSTRACT

Pathogenic microbial contamination is one of the causes of nosocomial infection in hospitals. Lemongrass oil and fennel oil are essential oils that are used as antimicrobials especially against Staphylococcus aureus. The purpose of this study was to determine the antibacterial activity of lemongrass oil and fennel oil against S. aureus isolated from Anwar Medika Sidoarjo hospital ward through the air diffusion method using essential oil diffuser. The compounds in lemongrass oil and fennel oil were analyzed by Gas Chromatography-Mass Spectrometer. The antibacterial activity test was conducted by the air capture method and continued with the Gram staining, catalase test, coagulase test, and calculation of the number of colonies. The results obtained from this study showed a decrease in the number of S. aureus colonies after the use of essential oils for 48 hours. Based on these results it can be concluded that lemongrass oil and fennel oil can suppress the growth of S. aureus bacteria isolated from hospital wards.

Keywords: antibacterial, lemongrass oil, fennel oil, S. aureus, hospital

ABSTRAK

Kontaminasi mikroba patogen adalah salah satu penyebab terjadinya infeksi nosokomial di rumah sakit. Minyak serai dapur dan minyak adas merupakan minyak atsiri yang dapat dimanfaatkan sebagai antimikroba khususnya pada bakteri Staphylococcus aureus. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri minyak serai dapur dan minyak adas terhadap S. aureus di ruang rawat inap rumah sakit melalui metode difusi udara dengan menggunakan Diffuser Essensial Oil. Kandungan senyawa dalam minyak sereh dapur dan adas dianalisa dengan GC-MS. Analisa aktivitas antibakteri menggunakan metode tangkap udara dan dilanjutkan uji perhitungan jumlah koloni, pewarnaan Gram, uji katalase, dan uji koagulase. Hasil yang diperoleh dari penelitian ini menunjukkan penurunan jumlah koloni S. aureus setelah penggunaan minyak atsiri dalam jangka waktu 48 jam. Berdasarkan hasil tersebut dapat disimpulkan bahwa minyak serai dapur dan minyak adas memiliki kemampuan untuk menekan pertumbuhan bakteri S. aureus dalam ruang perawatan inap rumah sakit kelas III.

Kata kunci: antibakteri, minyak serai dapur, minyak adas, S.aureus, rumah sakit

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