MANFAAT EKSTRAK ETANOL DAUN REMEK DAGING (Hemigraphis colorata W. Bull) TERHADAP LUKA BAKAR PADA TIKUS

Lusi Putri Dwita; Vera Ladeska; Aisyah Ramadhani; Dwi Rahma Augusta; Retno Tri Saufia

doi:10.22435/jtoi.v13i1.2823

Lusi Putri Dwita Universitas Muhammadiyah Prof. DR HAMKA
Vera Ladeska Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA Jakarta, Indonesia
Aisyah Ramadhani Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA Jakarta, Indonesia
Dwi Rahma Augusta Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA Jakarta, Indonesia
Retno Tri Saufia Fakultas Farmasi dan Sains, Universitas Muhammadiyah Prof. DR. HAMKA Jakarta, Indonesia

DOI: https://doi.org/10.22435/jtoi.v13i1.2823

Keywords: Hemigraphis colorata, burns, macrophages, fibroblasts, collagen

Abstract

ABSTRACT

Remek daging (Hemigraphis colorata W.Bull) have been studied and used traditionally for wound healing. This study aimed to determine the effect of topical application of remek daging leaves ethanolic extract 70% on the burn wound. The animals used for this study were 30 rats, divided into five groups, namely 20, 10, 5% remek daging extract ointment, negative control (vaseline flavum), and positive control (silver sulfadiazine 1%). Histology observations were held on days 3, 7, and 14 after burn wound induction. Histological observations showed an increase number of macrophages, fibroblasts, collagen density, and re-epithelialization in the extract ointment group significantly compare to the negative control (p <0.05). The application of ointment extract 20% to the rats showed comparable results to silver sulfadiazine 1% (p> 0.05). It can be concluded that remek daging ointment extract can accelerate the healing of burn wounds with the best results at a concentration of 20%.
Keywords: Hemigraphis colorata, burns, macrophages, fibroblasts, collagen.

ABSTRAK

Remek daging (Hemigraphis colorata W.Bull) telah diteliti dan digunakan untuk penyembuhan luka secara tradisional. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol 70% daun remek daging secara topikal pada luka bakar tikus putih. Hewan yang digunakan adalah 30 ekor tikus yang dibagi menjadi 5 kelompok yaitu kelompok salep ekstrak daun remek daging 20, 10, 5% (%b/b), kontrol negatif (vaselin flavum) dan kontrol positif (silver sulfadiazine 1% (%b/b)). Pengamatan secara histologi dilakukan pada hari ke 3, 7 dan 14 setelah induksi luka bakar. Pengamatan histologi menunjukkan peningkatan jumlah makrofag, jumlah fibroblas, kepadatan kolagen dan ketebalan re-epitelisasi pada kelompok salep ekstrak daun remek daging secara signifikan dibandingkan kontrol negatif (p<0,05). Tikus yang diberikan perlakuan salep ekstrak 20% menunjukkan hasil sebanding dengan silver sulfadiazin 1% (p>0,05). Dapat disimpulkan bahwa salep ekstrak daun remek daging dapat mempercepat penyembuhan luka bakar dengan hasil terbaik pada konsentrasi 20%.

Kata kunci: Hemigraphis colorata, luka bakar, makrofag, fibroblas, kolagen.

References

DAFTAR PUSTAKA

Al-aali, K. Y. (2016). Microbial Profile of Burn Wound Infections in Burn Patients, Taif , Saudi Arabia. Archives of Clinical Microbiology, 7(2), 1–9.

Departemen Kesehatan Republik Indonesia. (2008). Farmakope Herbal Indonesia Edisi I. Jakarta: Direktorat Jendral Pengawasan Obat dan Makanan. Hlm. 171-174.

Diegelmann, R. F. (2004). Wound Healing: an Overview of Acute, Fibrotic and Delayed Healing. Frontiers in Bioscience, 9(1–3), 283. https://doi.org/10.2741/1184

Fuadi, M. I., & Elfiah, U. (2015). Jumlah Fibroblas pada Luka Bakar Derajat ii pada Tikus dengan Pemberian Gel Ekstrak Etanol Biji Kakao dan Silver Sulfadiazine, e-Journal Pustaka Kesehatan, 3(2), 244–248.

Hanani E. (2015). Analisis Fitokimia. EGC, Jakarta. Hlm. 10-13, 69, 86, 125, 154, 239.

Harborne JB. (1987). Metode Fitokimia Penuntun Cara Modern Menganalisa Tumbuhan Terbitan Kedua. ITB Pers, Bandung.

Hesketh, M., Sahin, K. B., West, Z. E., & Murray, R. Z. (2017). Macrophage Phenotypes Regulate Scar Formation and Chronic Wound Healing, 1–10. https://doi.org/10.3390/ijms18071545

Joyson, A., & Krishnakumar, K. (2017). Hemigraphis colorata: a review, 6(4), 557–561.

Junqueira, L., & Carneiro, J. (2007). Basic Histology: Text & Atlas. The McGraw-Hill Companies.

Kalangi, S. J. R. (2013). Histofisiologi kulit. Jurnal Biomedik (JBM), 5(3), 12–20.

Kemenkes RI. (2018). Laporan Hasil Riset Kesehatan Dasar (Riskesdas) Indonesia tahun 2018. Riset Kesehatan Dasar 2018.

Kuhlmann, T., Bitsch, A., Stadelmann, C., Siebert, H., & Bru, W. (2001). Macrophages are Eliminated from the Injured Peripheral Nerve via Local Apoptosis and Circulation to Regional Lymph nodes and the Spleen, 21(10), 3401–3408.

Li, K., Diao, Y., Zhang, H., Wang, S., Zhang, Z., Yu, B., … Yang, H. (2011). Tannin Extracts from Immature Fruits of Terminalia Chebula fructus Retz. Promote Cutaneous Wound Healing in Rats. https://doi.org/10.1186/1472-6882-11-86

Moura, L. I. F., Dias, A. M. A., Suesca, E., Casadiegos, S., Leal, E. C., Fontanilla, M. R., … Carvalho, E. (2014). Neurotensin-loaded Collagen Dressings Reduce Inflammation and Improve Wound Healing in Diabetic Mice. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1842(1), 32–43. https://doi.org/10.1016/j.bbadis.2013.10.009

Nasiri, E., Hosseinimehr, S. J., Azadbakht, M., Akbari, J., Enayati-Fard, R., Azizi, S., & Azadbakht, M. (2015). The healing Effect of Arnebia Euchroma Ointment Versus Silver Sulfadiazine on Burn Wounds in Rat. World Journal of Plastic Surgery, 4(2), 134–144.

Pastar, I., Liang, L., Sawaya, A. P., Wikramanayake, T. C., Glinos, G. D., Drakulich, S., … Tomic-Canic, M. (2018). Preclinical Models for Wound-healing Studies. Skin Tissue Models for Regenerative Medicine. Elsevier Inc. https://doi.org/10.1016/B978-0-12-810545-0.00010-3

Prakashbabu, B. C., Vijay, D., & George, S. (2017). Wound Healing and Anti-inflammatory Activity of Methanolic Extract of Gmelina arborea and Hemigraphis colorata in Rats, 6(8), 3116–3122.

Rowan, M. P., Cancio, L. C., Elster, E. A., Burmeister, D. M., Rose, L. F., Natesan, S., … Chung, K. K. (2015). Burn Wound Healing and Treatment: Review and Advancements. Critical Care, 19(1), 1–12. https://doi.org/10.1186/s13054-015-0961-2

Thakur, R., Jain, N., Pathak, R., & Sandhu, S. S. (2011). Practices in Wound Healing Studies of Plants, 2011. https://doi.org/10.1155/2011/438056

Tiwari, vk. (2012). Burn Wound: How it Differs from other Wounds? Indian Journal of Plastic Surgery. https://doi.org/10.4103/0970-0358.101319

Verma, D. K., Bharat, M., Nayak, D., Shanbhag, T., Shanbhag, V., & Rajput, R. S. (2012). Areca Catechu: Effect of Topical Ethanolic Extract on Burn Wound Healing in Albino Rats. Int J Pharmacol and Clin Sci, 1(3), 74–78.

WHO. [May;2020 ];http://www.who.int/news-room/fact-sheets/detail/burns 2018

Young, A., & McNaught, C. E. (2011). The Physiology of Wound Healing. Surgery, 29(10), 475–479. https://doi.org/10.1016/j.mpsur.2011.06.011