KONFIRMASI KEBERADAAN Fasciola gigantica DAN HOSPES PERANTARA DI LINGKUNGAN PEMUKIMAN EKOSISTEM RAWA KABUPATEN HULU SUNGAI UTARA, KALIMANTAN SELATAN

  • Budi Hairani Balai Litbang P2B2 Tanah Bumbu
  • Syarif Hidayat Balai Litbang P2B2 Tanah Bumbu
  • Paisal Paisal Balai Litbang P2B2 Tanah Bumbu
Keywords: Fasciola gigantica, cercariae, freshwater snail

Abstract

Fasciola Gigantica infection occurs in swamp buffalo on  livestock in the swamp area of Hulu Sungai Utara District. Geographically, the livestock area is linked to the villages of Sungai Papuyu and Kalumpang Dalam  by swamp water with  no barrier. Such condition allows the snail as intermediate host of F. gigantica in livestock areas  easily spread to residential areas  and, therefore, raises the risk of transmission to humans. This study aimed to confirm the presence of F. gigantica in the cercariae form and snails  in the area around the settlement of both villages. The study was an observational study of cross-sectional design, conducted in August-December 2014 . Snail sampling was conducted at the Sungai Papuyu and Kalumpang Dalam village using hand collection method. To find cercariae in the snail  crushing technique was performed. Cercariae was confirmed by means of PCR to ensure they are F. gigantica in the Laboratory of Molecular Biology, Lambung Mangkurat University, Banjarbaru. The results in both villages showed 6 genera of snail, namely Pomacea, Bellamyâ , Indoplanorbis , Lymnaea , Gyraulus and Melanoides . There are 3 types of cercariae in the snails, namely Echinostome cercariae , Brevifurcate – pharyngeate cercariae and Sulcatomicrocercous cercariae. PCR shows positive samples of F. gigantica in the Echinostome cercariae form in Lymnaea and Indoplanorbis snails. This confirms the presence of F. gigantica cercariae around the settlements area and Lymnaea and Indoplanorbis snail are the first intermediate hosts.

References

Annida, 2013. Studi Komprehensif Epidemiologi Fasciolopsiasis dan Pemetaannya di Kabupaten Hulu Sungai Utara Provinsi Kalimantan Selatan Tahun 2012, Tanah Bumbu.
Annida;Paisal, 2014. Siput air tawar sebagai hospes perantara trematoda di Desa Kalumpang Dalam dan Sungai Papuyu , Kecamatan Babirik , Kabupaten Hulu Sungai Utara. Jurnal Buski, 5(2), pp.55–60.
Anuracpreeda, P., Chawengkirttikul, R. & Sobhon, P., 2016. Immunodiagnosis of Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease. Plos One, 11(1), p.e0145650.
Attwood, S.W. & Upatham, E.S., 2013. A Population Growth Trend Analysis for Neotricula aperta, the Snail Intermediate Host of Schistosoma mekongi, after Construction of the Pak-Mun Dam. PLoS Neglected Tropical Diseases, 7(11).
Byers, J.E. et al., 2013. Climate and pH Predict the Potential Range of the Invasive Apple Snail (Pomacea insularum) in the Southeastern United States. PLoS ONE, 8(2).
Chen, J.X. et al., 2013. An Outbreak of Human Fascioliasis gigantica in Southwest China. PLoS ONE, 8(8).
Grabner, D.S. et al., 2014. Invasion biology meets parasitology: A case study of parasite spill-back with egyptian Fasciola gigantica in the invasive snail Pseudosuccinea columella. PLoS ONE, 9(2), pp.1–8.
Hairani, B. et al., 2016. Identifikasi Serkaria Fasciolopsis buski dengan PCR untuk Konfirmasi Hospes Perantara di Kabupaten Hulu Sungai Utara, Kalimantan Selatan, Indonesia . Balaba, 12(1), pp.7–14.
Hairani, B., 2014. Laporan Hasil Penelitian : Deteksi Fasciolopsis buski pada Hospes Perantara (Keong Air Tawar) di Kabupaten Hulu Sungai Utara Melalui Metode PCR, Tanah Bumbu.
Hanington, P.C., Forys, M.A. & Loker, E.S., 2012. A somatically diversified defense factor, FREP3, is a determinant of snail resistance to schistosome infection. PLoS Neglected Tropical Diseases, 6(3).
Jayawardena, U. a, Rajakaruna, R.S. & Amerasinghe, P.H., 2011. Cercariae of trematodes in freshwater snails in three climatic zones in Sri Lanka. Ceylon Journal of Science (Biological Sciences), 39(2), pp.95–108.
Kulsantiwong, J. et al., 2013. Species-specific primers designed from RAPD products for Bithynia funiculata, the first intermediate host of liver fluke, Opisthorchis viverrini, in North Thailand. J Parasitol, 99(3), pp.433–7.
Le, T.H. et al., 2007. Molecular confirmation that Fasciola gigantica can undertake aberrant migrations in human hosts. Journal of Clinical Microbiology, 45(2), pp.648–650.
Lily Natalia;Suhardono;Adin Priadi, 2006. Kerbau Rawa Di Kalimantan Selatan : Permasalahan, Penyakit Dan Usaha Pengendalian. Wartazoa, 16(4), pp.206–215.
Lv, S. et al., 2013. Water-related parasitic diseases in China. International Journal of Environmental Research and Public Health, 10(5), pp.1977–2016.
Mas-Coma, S., Esteban, J.G. & Bargues, M.D., 1999. Epidemiology of human fascioliasis: a review and proposed new classification. Bull World Health Organ, 77, pp.340–346.
McDermott, K.S. et al., 2014. Distribution and occurrence of the exotic digenetic trematode (Centrocestus formosanus), its exotic snail intermediate host (Melanoides tuberculatus), and rates of infection of fish in springs systems in western Texas. The Southwestern Naturalist, 59(2), pp.212–220.
NODC Taxonomic Code database, 1996a. Fasciola gigantica Taxonomic Serial No.: 56136. Available at: https://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN&search_value=56136&print_version=PRT&source=to_print#null/%0A [Accessed January 17, 2017].
NODC Taxonomic Code database, 1996b. Fasciolopsis buski Taxonomic Serial No.: 56131. Available at: https://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN&search_value=56131&print_version=PRT&source=to_print#null/%0A [Accessed January 17, 2017].
Opisa, S. et al., 2011. Malacological survey and geographical distribution of vector snails for schistosomiasis within informal settlements of Kisumu City, western Kenya. Parasites & Vectors, 4(1), p.226.
Prasad, P.K. et al., 2011. PCR-based molecular characterization and insilico analysis of food-borne trematode parasites Paragonimus westermani, Fasciolopsis buski and Fasciola gigantica from Northeast India using ITS2 rDNA. Bioinformation, 6(2), pp.64–68.
Suhardono;Kosasih, 2000. Infeksi baru fasciola gigantica sebagai alat Untukmenetapkan strategi pengendalian fasciolosis Melalui pemberian flukisida pada kerbau rawa Di propinsi kalimantan selatan. In Seminar Nasional Peternakan dan Veteriner. Bogor: Balai Peneltian Veteriner Bogor, pp. 488–491.
Suhardono et.al, 2000. Dinamika populasi siput Lymnaea Rubiginosa dan kejadian Fasciolosis pada kerbau rawa di Kecamatan Danau Panggang. In Seminar Nasional Peternakan dan Veteriner. Bogor, pp. 492–497.
Suhardono et.al, 1999. Fasciolosis pada kerbau yang dipelihara pada lahan rawa di propinsi kalimantan selatan. In Seminar Nasional Peternakan dan Veteriner. Bogor: Balai Peneltian Veteriner Bogor, pp. 571–578.
Suon, S. et al., 2006. Seasonal differences in the incidence of infection with Fasciola gigantica in cambodian cattle. Tropical Animal Health and Production, 38(1), pp.23–28.
Yakhchali, M., Malekzadeh-Viayeh, R. & Imani-Baran, A., 2014. PCR-RFLP analysis of 28 SrDNA for specification of Fasciola gigantica (Cobbold, 1855) in the infected Lymnaea auricularia (Linnaeus, 1785) snails from Northwestern Iran. Iranian Journal of Parasitology, 9(3), pp.358–364.
Published
2018-06-30
How to Cite
Hairani, B., Hidayat, S., & Paisal, P. (2018). KONFIRMASI KEBERADAAN Fasciola gigantica DAN HOSPES PERANTARA DI LINGKUNGAN PEMUKIMAN EKOSISTEM RAWA KABUPATEN HULU SUNGAI UTARA, KALIMANTAN SELATAN. Vektora : Jurnal Vektor Dan Reservoir Penyakit, 10(1), 75-82. https://doi.org/10.22435/vk.v10i1.1054