Infeksi Virus Dengue pada Nyamuk Aedes aegypti Menggunakan Artificial Blood Feeding dan Deteksi Virus Dengue Menggunakan Teknik Molekular

  • Endang Srimurni Kusmintarsih Fakultas Biologi, Universitas Jenderal Soedirman, Jl. dr. Suparno 63, Purwokerto 53122, Indonesia
  • Medina Fadli Latus Syaadah
  • Edy Riwidiharso
  • R Tedjo Sasmono
Keywords: Artificial Blood Feeding, Aedes aegypti, Real Time (RT-PCR

Abstract

Abstract. Artificial blood-feeding using the parafilm-M membrane can be used as an alternative solution and
substitute live animals as a source of blood. This method is not only be used for blood-feeding but also to infect
the dengue virus (DENV) to mosquitoes. This study was aimed to determine the effectiveness artificial blood
feeding using parafilm-M membrane in Aedes mosquitoes originated in Indonesia and determine the positivity
of mosquitoes infected by Indonesia DENV-1. DENV-1 was isolated from patient and propagated in Vero cell
culture. The feeding was done in cardboard cups after mosquitos have been starved for 4-17 hours before being
fed with human blood. A conical 50ml tube was prepared, and a hole was created in the tube lid. The tube
opening was covered with parafilm. Glycerol was added into conical tube and heated in water bath for an hour
at 55oC. A mixture of blood and DENV-1 was made with concentration of 10%. Detection of DENV in bloodfed
mosquitos was carried out by using Simplexa Dengue Real-Time RT-PCR assay. The results showed that
the prevalence of blood-fed mosquitoes reached 66.67% with fasting period for 17 hours. Blood feeding
mosquitoes are affected by duration of fasting period, blood-feeding time, and attractants from human skin
rubbed into parafilm-M membrane. The prevalence of blood-fed Ae. aegypti infected by DENV was 20.83%.
This study provides information on the effectiveness of artificial parafilm membrane blood-feeding in a
laboratory setting that will be useful for vector control study in Indonesia.

Published
2019-12-27
How to Cite
1.
Kusmintarsih E, Latus Syaadah M, Riwidiharso E, Sasmono R. Infeksi Virus Dengue pada Nyamuk Aedes aegypti Menggunakan Artificial Blood Feeding dan Deteksi Virus Dengue Menggunakan Teknik Molekular. ASP [Internet]. 27Dec.2019 [cited 9Apr.2020];11(2):91-8. Available from: https://ejournal2.litbang.kemkes.go.id/index.php/aspirator/article/view/460