Aktivitas Larvasida Fraksi N-Heksan Ekstrak Etanol Daun Mengkudu (Morinda citrifolia. L) terhadap Larva Aedes sp.

Larvicide Activity of N-Hexane Fraction of Ethanolic Morinda citrifolia. L Leaves Extract on Aedes sp. Larvae

  • Rizki Awaluddin University of Darussalam Gontor
  • Binti Sholihatin University of Darussalam Gontor
  • Nurul Marfu'ah University of Darussalam Gontor
  • Solikah Ana Estikomah University of Darussalam Gontor
Keywords: Morinda citrifolia, larvicide, Aedes aegypti, n-hexane fraction, DHF


Abstract. Aedes sp. is a vector of the dengue virus that causes Dengue Hemorrhagic Fever (DHF).
Larvicides are the optimal method for controlling mosquito development. Temephos is a larvicidal
agent of the organophosphate group which is reported to cause side eff ects and ecological hazards,
as well as resistance based on reports in several country. This study aims to determine the larvicidal
activity of the n-hexane fraction of Morinda citrifolia leaf ethanol extract on Aedes sp. The compound
groups in the fraction were identifi ed using TLC through UV light and spray reagents. There were six
types of treatment including four concentration fractions (400, 600, 800, and 1000 ppm) as treatment,
positive control (temephos 1%) and negative control 1% acetone solution. Twenty-fi ve mosquito larvae
of Aedes sp. tested for each treatment. Larval mortality was recorded and LC50 and LC99 values were
analyzed using the probit. The results showed that the TLC test of the n-hexane fraction was positive
for terpenoids, anthraquinones, phenols, tannins, and fl avonoids. The results showed that the LC50 and
LC99 values were 1040 ppm and 2439 ppm. Therefore, the n-hexane fraction of the ethanol extract had
larvicidal activity on Aedes sp with li le toxicity.

Author Biographies

Binti Sholihatin, University of Darussalam Gontor

Department of Pharmacy, Faculty of Health Science, University of Darussalam Gontor

Nurul Marfu'ah, University of Darussalam Gontor

Department of Pharmacy, Faculty of Health Science, University of Darussalam Gontor

Solikah Ana Estikomah, University of Darussalam Gontor

Department of Pharmacy, Faculty of Health Science, University of Darussalam Gontor


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How to Cite
Awaluddin R, Sholihatin B, Marfu’ah N, Estikomah S. Aktivitas Larvasida Fraksi N-Heksan Ekstrak Etanol Daun Mengkudu (Morinda citrifolia. L) terhadap Larva Aedes sp. ASP [Internet]. 28Dec.2021 [cited 21May2022];13(2):137-46. Available from: https://ejournal2.litbang.kemkes.go.id/index.php/aspirator/article/view/4823