PERFORMA Tryptone Bile X-Glucuronide (TBX) YANG DISUPLEMENTASI CEFOTAXIME SEBAGAI MEDIUM SELEKTIF UNTUK SKRINING ESBL-E.coli DARI SAMPEL LINGKUNGAN

  • Tati Febrianti
Keywords: Escherichia coli, ESBL, TBX Agar suplemented with Cefotaxime

Abstract

Escherichia coli (E.coli) is one of the gram-negative bacteria producing Extended-spectrum β-lactamase (ESBL) which is the focus of global health problems related to resistance. Environmental contamination by ESBL-E.coli can be found in river, around slaughterhouses and hospital waste. Identification of ESBL-E.coli from environment will be challenging to do considering that E. coli mixes with various other microorganisms in the environment. This study aimed to determine the performance of TBX Agar supplemented with Cefotaxime as a selective medium for ESBL-E.coli screening. The research samples were 138 water samples of environmental with a volume of 100 ml obtained from rivers, open sewers in the market, Poultry Slaughterhouses (RPHU) and hospital waste inlets and outlets around Jakarta. Laboratory examinations were carried out through the filtration stage, inoculation on the TBX Agar+Cefotaxime medium as well as species confirmation and ESBL with the indol test and double-disk test. The results showed that 87,08% (40-100%) of suspect colonies growing on TBX Agar+Cefotaxime medium were confirmed as E.coli and 82,51% (12-100%) were confirmed as ESBL-E.coli. There was no correlation between TBX Agar+Cefotaxime performance and sampling locations. Based on the results of the study, it can be concluded that the TBX+Cefotaxime medium can be used for ESBL-E.coli screening in the environment, but further confirmation is needed using the indole and tests.

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Published
2021-04-05
How to Cite
1.
Febrianti T. PERFORMA Tryptone Bile X-Glucuronide (TBX) YANG DISUPLEMENTASI CEFOTAXIME SEBAGAI MEDIUM SELEKTIF UNTUK SKRINING ESBL-E.coli DARI SAMPEL LINGKUNGAN. bpk [Internet]. 5Apr.2021 [cited 6May2021];. Available from: https://ejournal2.litbang.kemkes.go.id/index.php/bpk/article/view/3858