Keragaman Bakteri Endofit Penghasil L-Asparaginase Bebas L-Glutaminase

  • tri ratna sulistiyani Research Center for Biology (LIPI)
  • dinihari indah kusumawati
Keywords: Endhophytic bacteria, L-asparaginase, Medical plants


Endophytes are potential as a source of active compound producer. Endophytes that is isolated from tropical medicinal plants has been getting attention due to its high biodiversity and active compound producing ability. L-asparaginase is the first enzyme used as chemotherapeutic agent for leukemia. The aim of this study is to collect the endophytic bacteria associated with tropical medicinal plants from Sumba Island and investigate the activity of L-asparaginase without L-glutaminase from endophytic bacteria isolates. The samples Gliricidia sepium, Pittosporum moluccanum, Clerodendrum buchanani, and Zingiberaceae are collected from Wanggameti, East Sumba, NTT. Samples are sterilized using surface-sterilization method and endophytic bacteria are isolated using plant piece method on R2A media. Selected endophytic bacteria are identified by 16S rDNA sequences. L-asparaginase screening is conducted using modified R2A with addition of L-asparagine and phenol red as colour indicator. A total of 34 isolates of endophytic bacteria were collected from 5 samples. A total of 14 genus consisted of 17 different bacterial species were obtained from 34 selected isolates. Endophytic bacteria of P. stutzeri strains of SMKL1 and R. radiobacter strains of SMKW2 from the Kahili plant were needed as L-glutaminase-free L-asparaginase and were potential to be candidate of leukemia cancer chemotherapy


Bray F, Ferlay J, Soerjomataram I. Global Cancer Statistics 2018 : GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries. 2018;394–424.

Wahidin M, Noviani R, Hermawan S, Andriani V, Ardian A, Djarir H. Population-Based Cancer Registration in Indonesia. 2012;13:1709–10.

Dixit S, Ali H. Anticancer activity of Medicinal plant extract-A review. J Chem Cheml Sci. 2010;1(1):79–85.

Suhirman S, Hernani, Syukur C. Uji toksisitas ekstrak lempuyang gajah (Zingiber zerumbet) terhadap larva udang (Artemia salina Leach.) Bul Littro. 2006;XVII(1):30–8.

Devita VT, Chu E. AACR Centennial Series A History of Cancer Chemotherapy. 2008;(21):8643–54.

Hu Q, Sun W, Wang C, Gu Z. Recent advances of cocktail chemotherapy by combination drug delivery systems. Adv Drug Deliv Rev 2015; Available from:

Tan RX, Zou WX. Endophytes: a rich source of functional metabolites. Nat Prod Rep. 2001;18(4):448–59.

Vellard M. The enzyme as drug: Application of enzymes as pharmaceuticals. Curr Opin Biotechnol. 2003;14(4):444–50.

Manasa C, Nalini MS. L-Asparaginase Activity of Fungal Endophytes from Tabernaemontana heyneana Wall . (Apocynaceae), Endemic to the Western Ghats (India). Int Sch Res Not. 2014;2014:1–7.

Joshi RD, Kulkarni NS. Optimization studies on L-asparaginase production from endophytic Bacteria. 2016;2:624–9.

Saxena A, Upadhyay R, Kango N. Isolation and identification of actinomycetes for production of novel extracellular glutaminase free L-asparaginase. Indian J Exp Biol. 2015;53(12):786–93.

Jalgaonwala RE, Mahajan RT. Evaluation of hydrolytic enzyme activities of endophytes from. Biotechnol Adv. 2011;7(6):1733–41.

Arau WL, Marcon J, Maccheroni W, Elsas JD Van, Vuurde JWL Van. Diversity of Endophytic Bacterial Populations and Their Interaction. 2002;68(10):4906–14.

Powers EM. Efficacy of the Ryu nonstaining KOH technique for rapidly determining gram reactions of food-borne and waterborne bacteria and yeasts. Appl Environ Microbiol. 1995;61(10):3756–8.

Packeiser H, Lim C, Balagurunathan B, Wu J, Zhao H. An extremely simple and effective colony PCR procedure for bacteria, yeasts, and microalgae. Appl Biochem Biotechnol. 2013;169(2):695–700.

Palaniappan P, Chauhan PS, Saravanan VS, Anandham R, Sa T. Isolation and characterization of plant growth promoting endophytic bacterial isolates from root nodule of Lespedeza sp. Biol Fertil Soils. 2010;46(8):807–16.

Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program fro Windows 95/98/NT. Nucleic Acids Symp Series. 1999;41:95-98

Kim OS, Cho YJ, Lee K, Yoon SH, Kim M, Na H, et al. Introducing EzTaxon-e: A prokaryotic 16s rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol. 2012;62(PART 3):716–21.

Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evo. 1987;4:406–25.

Kumar S, Stecher G, Tamura K. MEGA7 : Molecular Evolutionary Genetics Analysis Version 7 . 0 for Bigger Datasets. 2018;33(7):1870–4.

Mahajan R V., Saran S, Saxena RK, Srivastava AK. A rapid, efficient and sensitive plate assay for detection and screening of l-asparaginase-producing microorganisms. FEMS Microbiol Lett. 2013;341(2):122–6.

Ruby EJ. A Review: Bacterial Endophytes and their Bioprospecting. Journal of Pharmacy Research, 2011;4(3), pp.795–799.

Chareprasert S, Piapukiew J, Thienhirun S, Whalley AJS, Sihanonth P, Products F. Endophytic fungi of teak leaves Tectona grandis L . and rain tree leaves Samanea. 2006;481–2.

Chow Y, Ting ASY. Endophytic l-asparaginase-producing fungi from plants associated with anticancer properties. J Adv Res. 2015;6(6):869–76.

Chowdhury EK, Jeon J, Rim SO, Park Y. Composition , diversity and bioactivity of culturable bacterial endophytes in mountain-cultivated ginseng in Korea. Sci Rep. 2017;(July):1–10. Available from:

Hardoim PR, Overbeek LS Van, Berg G, Pirttilä M, Compant S, Campisano A, et al. The Hidden World within Plants : Ecological and Evolutionary Considerations for Defining Functioning of Microbial Endophytes. 2015;79(3):293–320.

Ratna Sulistiyani T, Lisdiyanti P, Lestari Y. Population and Diversity of Endophytic Bacteria Associated with Medicinal Plant Curcuma zedoaria. Microbiol Indones. 2014;8(2):65–72. Available from:

Lopes AM, Oliveira-Nascimento L de, Ribeiro A, Tairum CA, Breyer CA, Oliveira MA de, et al. Therapeutic l-asparaginase: upstream, downstream and beyond. Crit Rev Biotechnol. 2017;37(1):82–99.

Parte AC, Road W. LPSN — list of prokaryotic names with standing in nomenclature. 2014;42(November 2013):613–6.

How to Cite
sulistiyani tri, kusumawati dinihari. Keragaman Bakteri Endofit Penghasil L-Asparaginase Bebas L-Glutaminase. jki [Internet]. 16May2019 [cited 27Mar.2023];9(1):28-9. Available from: