Efektivitas Formula Krim Tabir Surya Berbahan Aktif Ekstrak Etanol Biji Wali (Brucea javanica L. Merr)

  • Almira Amini Universitas Mataram
  • Candra Dwipayana Hamdin Universitas Mataram
  • Handa Muliasari
  • Windah Anugrah Subaidah
Keywords: Formulation, Effectivity test, Sunscreen cream, Wali seed extract, Erythema

Abstract

High intensity of ultraviolet (UV) radiation causes several adverse effect such as erythema, sunburn, pigmentation, early senescence, and skin cancer. These effects can be prevented by using sun protection product. Wali (Brucea javanica L. Merr) is one of natural plants that contain phenol and flavonoid. These compounds are proven to have sunscreen effect. The purposes of this studies are to formulate and evaluate the effectivity of oil-in-water-type sunscreen cream using 1% ethanol extract of Wali seed. The sunscreen cream is developed by using a fusion method and the evaluation of the effectivity is conducted in vivo on the skin of mice (Mus musculus). The formulated sunscreen cream is then tested for its physical properties, including homogenity, pH, spreadability, adhesion, and stability. The effectivity of the formulated cream is also tested by observing the formation of erytema on the skin of mice (Mus musculus). Erythema appearing on the mouse skin is analyzed with ImageJ and SPSS v23 using the one way anova method. The results showed that the ethanol extract of Wali seeds could be formulated into sunscreen cream. Furthermore, the formulated cream was also able to protect the mice skin against the formation of erythema significantly compared to negative controls (p <0.05) after exposure to UV B light by 93% erythema inhibition activity

References

Ahmad, Shamim I, editors. Ultaviolet light in human health, diseases, and environment. Switzerland: Springer; 2017.

McKenzie R. UV radiation in the melanoma capital of the world: What makes New Zealand so different? American Institute of Physics Conferency Proceedings. 2017;1810(1):1–8.

Saewan N, Jimtaisong A. Photoprotection of natural flavonoids. Journal of Applied Pharmaceutical Science. 2013;3(9):129–41.

Isfardiyana S, Safitri S. Pentingnya melindungi kulit dari sinar ultraviolet dan cara melindungi kulit dengan sunblock buatan sendiri. Jurnal Inovasi dan Kewirausahaan. 2014;3(2):126–33.

Ahmad I, Agus ASR. Uji stabilitas formula krim tabir surya ekstrak umbi bawang dayak (Eleutherine americana L. Merr.). Journal of Tropical Pharmacy and Chemistry. 2013; 2(3):159–65.

Latha MS, Martis J, Shobha V, Sham SR, Bangera S, Krishnankutty B, et al. Sunscreening agents: A review. The Journal of Clinical Aesthetic Dermatology. 2013; 6(1):16–26.

Fauzi AR, Rina N. Merawat kulit dan wajah. Jakarta: Gramedia; 2012.

Gadri A, Darijono ST, Mauludin R, Iwo MI. Formulasi sediaan tabir surya dengan bahan aktif nanopartikel cangkang telur ayam broiler. Jurnal Matematika dan Sains. 2012;17(3):89–97.

Hogade MG, Patil B, Prashant D. Comparative sun protection factor determination of fresh fruits extract of cucumber vs marketed cosmetic formulation. Research Journal of Pharmaceutical Biological and Chemical Sciences. 2010;1(3):55–9.

Hamdin CD, Muliasari H, Ihsan M. Efek hipoglikemis suspensi biji buah Wali (Brucea javanica (L.) Merr pada tikus Diabetes. BioWallace JIIB. 2017;3(2):88-93.

Ablat A, Mohamad J, Awang K, Shilpi JA, Arya A. Evaluation of antidiabetic and antioxidant properties of Brucea javanica seed. The Scientific World Journal:2014:1–8.

Helmi H, Susanti I, Agung NA, Kusen S. Antibacterial activity of belilik (Brucea javanica (L). Merr) growth of enteropathogenic bacteria. Journal of Biological Research. 2015. 21(1):35–40.

Bagheri E, Hajiaghaalipour F, Nyamathulla S, Salehen N. The apoptotic effects of Brucea javanica fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation. Dovepress. 2018;12:657–71.

Alhabsyi DF, Suryanto E, Wewengkang DS. Aktivitas antioksidan dan tabir surya pada ekstrak kulit buah pisang goroho (Musa acuminate L.). Pharmacon. 2014;3(2):107-14.

Achmad, SA, Hakim EH, Makmur L, Syah YM, Juliawaty LD, Mujahidin D. Ilmu kimia dan kegunaan tumbuh tumbuhan obat indonesia jilid 1. Bandung: Penerbit ITB; 2009.

Muliasari H, Hamdin CD, Ananto AD IM. Hypoglycemic effect of Brucea javanica (L) Merr leaves and seed extract in alloxan-induced diabetic rats. Proceeding of 2nd International Conference on Science and Technology. 2017:62–7.

Dewi R, Anwar E, Yunita KS. Uji stabilitas fisik formula krim yang mengandung ekstrak kacang kedelai (Glycine max). Pharmaceutical Sciences and Research. 2014. 1(3):194–208.

Yumas M. Formulasi sediaan krim wajah berbahan aktif ekstrak metanol biji kakao non fermentasi (Theobroma cacao L) kombinasi madu lebah. Jurnal Industri Hasil Perkebunan. 2016; 11(2):75–87.

Azkiya Z, Ariyani H, Nugraha T. Evaluasi sifat fisik krim ekstrak jahe merah (Zingiber officinale Rosc. var. rubrum) sebagai antinyeri. Journal of Current Pharmaceutical Sciences. 2017;1(1):12–8.

Oktaviasari L, Zulkarnain A. Formulasi dan uji stabilitas fisik sediaan lotion o/w pati kentang (Solanum Tuberosum L.) serta aktivitasnya sebagai tabir surya. Majalah Farmaseutik. 2017;13(1):9–27.

Eff ARY, Pertiwi RD, Rakhmawati I, Utami TP. In-vitro and in-vivo sunscreen activity of active compounds isolated from fruits of Phaleria marcocarpha (Scheff.) Boerl. Journal of Young Pharmacist. 2018;10(2s):S106–10.

Rao USM, Abdurrazak M, Mohd KS. Phytochemical screening, total flavonoid and phenolic content assays of various solvent extracts of tepal of musa paradisiaca penyaringan fitokimia, jumlah asai kandungan flavonoid dan fenolik pelbagai ekstrak pelarut tepal Musa paradisiaca. Malaysian Journal of Analytical Sciences. 2016; 20(5):1181–90.

Ramakrishna A, Ravishankar G. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior. 2011;6(11):1720–31.

Rahayu MP, Wiryosoendjoyo K, Prasetyo A. Uji aktivitas antibakteri ekstrak soxhletasi dan maserasi buah makassar (Brucea javanica L. Merr) terhadap bakteri Shigella dysentriae ATCC 9361 secara in-vitro. Jurnal Biomedika. 2012. 2(1).

Hasni KU, Lubees ZIZ, Mohamad J, Varman M. Brucea javanica seeds as source of potential natural antioxidants to improve biodisel thermal and oxidative stability. Malaysian Journal of Fundamental and Applied Sciences. 2017;13(3):207-12.

Pandiangan CPP. Aktivitas buah makassar (Brucea javanica (L.) Merr) sebagai antikanker. Journal of agromedicine unila. 2015;2(2):113-7.

Agarwal P. Pharmaceutical calculations. New York: Jones & Bartlett Learning; 2016.

Butler H. Poucher's perfumes, cosmetics and soaps 10th edition. Dordretcht. Kluwer Academic Publisher; 2000.

Ulaen SPJ, Banne Y SR. Pembuatan salep anti jerawat dari ekstrak rimpang temulawak (Curcuma xanthorrhiza Roxb.). Jurnal Ilmiah Farmasi. 2012;3(2):45–9.

Shankar R, Sarangi B, Gupta R, Pathak K. Formulation and characterization of polyherbal cream for skin manifestations. Journal of Asian Association of Schools of Pharmacy. 2016;1:360–6.

Roberths SM, James RC, Williams PL. Principles of toxicology environmental and industrial applications third edition, New Jersey: John Wiley & Sons; 2015.

Grimes PE. Aesthetic and cosmetic surgery for darker skin type. Philadephia: Lippincot Williams & Wilkins; 2008.

Rhodes L, Gledhill K, Masoodi M, Haylett A, Brownrigg M, Thody A, et al. The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases. The Federation of American Scienties for Experimental Biology Journal. 2009; 23(11):3947–56.

Ruzicka, T. Eicosanoids and the Skin. Florida. CRC Press; 2000.

Rhodes L, Belgi G, Parslew R, McLoughlin L, Clough G, Friedmann P. Ultraviolet-B-induced erythema is mediated by nitric oxide and prostaglandin E2 in combination. Journal of Investigate Dermatology. 2001;117(4):880–5.

Auerbach, P, Cushing T, Harris NS. Auerbach’s wilderness medicine seventh edition. New York: Elsevier Health Sciences; 2016.

Elmets CA, Ledet J, Athar M. Cyclooxygenases: Mediators of UV-induced skin cancer and potential targets for prevention. Journal of Investigate Dermatology. 2014;134(10):2497-502.

Afaq S, Katiyar SK. Polyphenols: skin photoprotection and inhibition of photocarcinogenesis. Mini-Reviews in Medicinal Chemistry. 2011;11(14):1200–15.

Cetinkaya H, Kulak M, Karaman M, Karaman HS, Kocer F. Flavonoid accumulation behavior in response to the abiotic stress: can a uniform mechanism be illustrated for all plants ?. Intech Open Scienc. 2017. 151-65.

Sisa M, Bonnet SL, Ferreira D, Van Der Westhuizen JH. Photochemistry of flavonoids. Molecules. 2010; 15(8):5196–245.

Published
2020-02-24
How to Cite
1.
Amini A, Hamdin C, Muliasari H, Subaidah W. Efektivitas Formula Krim Tabir Surya Berbahan Aktif Ekstrak Etanol Biji Wali (Brucea javanica L. Merr). jki [Internet]. 24Feb.2020 [cited 3Aug.2020];10(1):50-8. Available from: https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/2066