Preparation of Curcumin-Liposomes using pH-Driven Method to Enhance Stability and Encapsulation Efficiency
AbstractCurcumin, a hydrophobic polyphenol, derived from turmeric, has numerous pharmacological activities, including hepatoprotective, antioxidant, anti-inflammatory, antimicrobial, and anticarcinogenic. However, the therapeutic efficacy of curcumin is limited due to its poor oral bioavailability and high susceptibility to degradation. The poor oral bioavailability of curcumin has been attributed to its poor aqueous solubility and extensive first past metabolism. Curcumin dissolves in alkaline conditions but it is highly unstable undergoing rapid hydrolytic degradation in neutral or alkaline condition. To solve these problems, liposome-based encapsulation technology is applied to improve the bioavailability of curcumin. The existing method to prepare curcumin-liposome, thin-film method, is complex and usually required the use of organic solvents. In this study, pH-driven method, an organic solvent-free and easily encapsulation technique utilizing the pH-dependent solubility of curcumin was proposed to improve the stability and bioavailability. Curcumin-liposomes were prepared using phospholipon 90 H and soya lecithin as phospholipid sources. The characterizations of curcumin-liposomes include particle size, microstructure, encapsulation efficiency, infrared spectra, salt stability and storage stability. The particle size measured was 77.85 ± 0.39 nm. It was found that curcumin-liposomes prepared by the pH-driven method was stable during storage for 30 days and gave encapsulation efficiency of 60.71 ± 0.20%, higher as compared to the ones prepared by thin-film method. In conclusion, pH-driven is a promising method for the preparation of curcumin-liposomes to enhance stability and encapsulation efficiency
Konatham S, Nyathani HK, Bonepally CR, Yeannameneni PK, Aukunuru J. Liposomal delivery of curcumin to liver. Turkish Journal of Pharmaceutical Sciences. 2010;7(2):89–98.
Cheng C, Peng S, Li Z, Zou L, Liu W, Liu C. Improved bioavailability of curcumin in liposomes prepared using a pH-driven, organic solvent-free, easily scalable process. Royal Society of Chemistry Advances. 2017;7(42):25978–86.
Feng T, Wei Y, Lee RJ, Zhao L. Liposomal curcumin and its application in cancer. International Journal of Nanomedicine. 2017;12:6027–44.
Lu Y, Ding N, Yang C, Huang L, Liu J, Xiang G. Preparation and in vitro evaluation of a folate-linked liposomal curcumin formulation. Journal of Liposome Research. 2012;22(2):110–9.
He H, Lu Y, Qi J, Zhu Q, Chen Z, Wu W. Adapting liposomes for oral drug delivery. Acta Pharmaceutica Sinica B. 2019;9(1):36–48.
Laouini A, Charcosset C, Fessi H, Holdich RG, Vladisavljević GT. Preparation of liposomes: a novel application of microengineered membranes-from laboratory scale to large scale. Colloids and Surfaces B: Biointerfaces. 2013;112:272–8.
Pan K, Luo Y, Gan Y, Baek SJ, Zhong Q. pH-driven encapsulation of curcumin in self-assembled casein nanoparticles for enhanced dispersibility and bioactivity. Soft Matter. 2014;10(35):6820–30.
Basnet P, Hussain H, Tho I, Skalko-Basnet N. Liposomal delivery system enhancesanti-inflammatory properties of curcumin. Journal of Pharmaceutical Sciences. 2012;101(2):598-609.
Peng S, Zou L, Liu W, Li Z, Liu W, Hu X, et al. Hybrid liposomes composed of amphiphilic chitosan and phospholipid: Preparation, stability and bioavailability as a carrier for curcumin. Carbohydrate Polymers. 2017;156:322–32.
Kumar N, Rai A, Reddy ND, Raj PV, Jain P, Deshpande P, et al. Silymarin liposomes improves oral bioavailability of silybin besides targeting hepatocytes, and immune cells. Pharmacological Reports. 2014;66(5):788–98.
Chen X, Zou LQ, Niu J, Liu W, Peng SF, Liu CM. The stability, sustained release and cellular antioxidant activity of curcumin nanoliposomes. Molecules. 2015;20(8):14293–311.
Patil YP, Jadhav S. Novel methods for liposome preparation. Chemistry and Physics of Lipids. 2014;177:8–18.
Hasan M, Ben Messaoud G, Michaux F, Tamayol A, Kahn CJF, Belhaj N, et al. Chitosan-coated liposomes encapsulating curcumin: study of lipid-polysaccharide interactions and nanovesicle behavior. Royal Society of Chemistry Advances. 2016;6(51):45290–304.
Yallapu MM, Jaggi M, Chauhan SC. β-Cyclodextrin-curcumin self-assembly enhances curcumin delivery in prostate cancer cells. Colloids and Surfaces B: Biointerfaces. 2010;79(1):113–25.
Zou L, Peng S, Liu W, Gan L, Liu W, Liang R, et al. Improved in vitro digestion stability of (-)-epigallocatechin gallate through nanoliposome encapsulation. Food Research International. 2014;64:492–9.
Paramera EI, Konteles SJ, Karathanos VT. Microencapsulation of curcumin in cells of Saccharomyces cerevisiae. Food Chemistry. 2011;125(3):892–902.
Liu W, Liu W, Ye A, Peng S, Wei F, Liu C, et al. Environmental stress stability of microencapsules based on liposomes decorated with chitosan and sodium alginate. Food Chemistry. 2016;196:396–404.
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