Uji Aktivitas Antioksidan dan Penghambatan Tirosinase serta Uji Manfaat Gel Ekstrak Kulit Batang Taya (Nauclea subdita) terhadap Kulit
AbstractDayak women in Borneo treat their skin with natural ingredients, one of which is taya cortex (Nauclea subdita). Flavonoids that can be found in natural ingredients have antioxidant effect and can inhibit the activity of tyrosinase in melanogenesis process. The research objectives were to measure the antioxidant activity and the inhibition of tyrosinase of taya cortex extract, to obtain a stable formulation of taya cortex extract gel, and to determine the safety and efficacy on human skin. The method used for antioxidant activity test was DPPH method. The inhibition of tyrosinase was conducted with kojic acid as control. The safety and efficacy test were conducted to healthy women ages 35-50 with healthy and normal skin. The results show IC50 value of 568.58 µg/mL in L-tyrosine and 1374.69 µg/mL in L-DOPA for inhibiton of tyrosinase. Antioxidant activity assay of the extract shows IC50 value of 48.78 µg/mL and can be categorized as a powerful antioxidant (<50 µg/mL). The formulation of gel containing taya cortex extract was physically stable for 12 weeks. Safety test and efficacy test of the gel show the gel is safe to use topically and show an increase in skin elasticity.
Riwut T, Mantikei S. Maneser Panatau Tatu Hiang – Menyelami Kekayaan Leluhur. Editor Nila Riwut. Pusakalima. Palangkaraya. 2008.
Lin J.Y, Fisher D.E. Melanocyte biology and skin pigmentation. Nature Journal. 2007;445(7130):843-50.
Sheo SY, Sharma VK, Sharma N. Mushroom Tyrosinase: Recent Prospects. Journal of Agricultural Food Chemistry. 2003;51(10):2837-53.
Uyen LDP, Nguyen DH, Kim EK. Mechanism of skin pigmentation. Biotechnology and Bioprocess Engineering, 2008; 13(4);383-95.
Fitrie AA. Histologi dari Melanosit. Fakultas Kedokteran Bagian Histologi, Universitas Sumatera Utara. 2004.
Ohguchi K, Tanaka T, Kido T.. Effects of hydroxystilbene derivatives on tyrosinase activity. Biochemistry Biophysical Research Community. 2003;307(4):861-63.
Pinnell SR. Cutaneous photodamage, oxidative stress, and topical antioxidant protection. J Am Acad Dermatol, 2003; 48(1),1-19.
Indonesia. Farmakope Herbal Indonesia Edisi I, Jakarta. Departemen Kesehatan; 2008.
Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002;10:178-82.
Ramamoorty, Praveen K, Bono A. Antioxidant activity total phenolic and flavonoid content of Morinda citrifolia fruit extracts from various extraction processes. Journal of Engineering and Technology. 2007;2(1):70-80.
Batubara I, Darusman L K, Mitsunaga T, Rahminiwati M, Djauhari E. Potency of Indonesia medicinal plant as tyrosinase inhibitor and antioxidant agent. J. Biol Sci. 2010;10(2):138-44.
Spiewak R. Patch testing for contact allergy and allergic contact dermatitis. The Open Allergy Journal 2008;1:42-51.
Lusivera TK. Mempelajari pengaruh pemanasan terhadap kadar flavonoid [Skripsi]. Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Bogor. 2002.
Fidrianny I., darmawati A., Sukrasno.. Antioxidant capacities from different polarities etracts of Cucurbitaceae Leaves Using FRAP, DPPH assay and correlation with phenolic, flavonoid, carotenoid content. Int. J. Pharm. Pharm. Sci. 2014;6(2):858-62.
Yanhouy L, Hong CO, Nam MH, Kim JH. Antioxidant and Glycation Inhibitory Activities of Gold Kiwifruit, Actinidia chinensis. J. Korean Soc. Appl. Biol. Chem. 2011;54(3):460-67.
Momtaz S, Mapunya BM, Houghton PJ, Edgerly C, Hussein A, Naidoo S, et.al. Tyrosinase inhibition by extracts and constituents of Sideroxylon inerme L. stem bark, used in South Africa for skin lightening.Journal of Ethnopharmacology. 2008;119(3):507–12.
Hyuk SJ, Hyun PS, Hwan SD, Hee LJ, Won SW, Sun HS. Antioxidative and Skin-Whitening Effect of an Aqueous Extract of Salicornia herbacea. Bioscience, Biotechnology, and Biochemistry. 2009;73(3):552-56.
Copyright (c) 2016 Jurnal Kefarmasian Indonesia
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.