Preparasi Radiofarmaka Nanokoloid Human Serum Albumin (HSA) untuk Limfosintigrafi

  • Yunilda Yunilda Pusat Teknologi Radioisotop dan Radiofarmaka, Badan Tenaga Atom dan Nuklir, Indonesia
  • Rohadi Awaludin Pusat Teknologi Radioisotop dan Radiofarmaka, Badan Tenaga Atom dan Nuklir, Indonesia
Keywords: Sentinel lymph node, Lymphoscintigraphy, Radiopharmaceuticals, Nanoparticle, Human serum albumin


Nanocolloid human serum albumin (HSA) labeled with technetium-99m is widely used in nuclear medicine for detection of breast cancer through examination of sentinel lymph node (SLN). SLN examination is used to detect the spread of cancer cells before and after surgery. Accumulation of a radiopharmaceutical in lymph nodes was detected by using a gamma cameraor SPECT. The aim of this study is to establish the protocol of HSA nanocolloid preparation with composition and specification in accordance with the Technical Leaflet Nanocoll Albumon which refers to The Society of Nuclear Medicine and Molecular Imaging (SNMMI) and European Association of Nuclear Medicine (EANM).This study includes the step of preparation and quality test. The method was carried out by means of protein denaturation of HAS at certain pH and heating at certain temperature. Quality tests were carried out using particle size analyzer (PSA) to determine the particle size, Transmission Electron Microscopy (TEM) to observe the morphology of the particles, and paper chromatography to measure radiolabeling yield. This study has obtained the particle size 80% of <100 nm, the yieldof morphology labelling >95% and pH of 7.3–7.4. The result was a protocol of preparing HSA nanocolloid.


Buletin Jendela Data dan Informasi Kesehatan. Jakarta: Kementerian Kesehatan RI;2015.

Tokin CA, Cope FO, Metz WL, Blue MS, Potter BM, Abbruzzese BC, et al. The efficacy of Tilmanocept in sentinel lymph mode mapping and identification in breast cancer patients: a comparative review and meta-analysis of the 99mTc-labeled nanocolloid human serum albumin standard of care. Clin Exp Metastasis. 2012;29(7):681–6.

Vidal-Sicart S, Valdés Olmos R. Sentinel node mapping for breast cancer: Current situation. J Oncol. 2012;1-7.

Kraft O, Havel M. Sentinel lymph nodes and planar scintigraphy and SPECT/CT in various types of tumours. Estimation of some factors influencing detection success. Nucl Med Rev. 2013;16(1):17–25.

Brouwer O, Buckle T, Vermeeren L, Klop W, Balm A, Van Der Poel HG, et al. Comparing the hybrid fluorescent-radioactive tracer indocyanine green- 99mTc-Nanocolloid with99mTc-nanocolloid for sentinel node identification: A validation study using lymphoscintigraphy and SPECT/CT. J Nucl

Med [Internet]. 2012;53(7):1034–40. Available from:

Van Den Berg NS, Buckle T, Kleinjan GI, Klop WM, Horenblas S, Van Der Poel HG, et al. Hybrid tracers for sentinel node biopsy. Q J Nucl Med Mol imaging Off Publ Ital Assoc Nucl Med [and] Int Assoc Radiopharmacol (IAR), [and] Sect Soc Radiopharm. 2014;58(2):193–206.

Heuveling DA, Visser GWM, De Groot M,De Boer JF, Baclayon M, Roos WH, et al. Nanocolloidal albumin-IRDye 800CW: A near-infrared fluorescent tracer with optimal retention in the sentinel lymph node. Eur J Nucl Med Mol Imaging. 2012;39(7):1161–8.

Schiller E, Bergmann R, Wunderlich G, Andreeff M, Jacob A, Pietzsch H-J. Ga-68-and Cu-64-Labeled NOTA-Albumin Conjugates for PET Sentinel Lymph Node Imaging. ISRN Mol Imaging [Internet].

;2013:1–8. Available from:


Wallace AM, Han LK, Povoski SP, Deck K, Schneebaum S, Hall NC, et al. Comparative evaluation of [(99m)tc]tilmanocept for sentinel lymph node mapping in breast cancer patients: results of two phase 3 trials. Ann Surg Oncol [Internet]. 2013;20(8):2590–9. Available from: http://www.pubmed 144&tool=pmcentrez&rendertype=abstract

Widyasari EM, Oekar NK. Formulasi kit Human Serum Albumin (HSA)-nanosfer sebagai radiofarmaka untuk studi limfosintigrafi di kedokteran nuklir. 2012;13(1):49-60.

Giammarile F, Alazraki N, Aarsvold JN, Audisio RA, Glass E, Grant SF, et al. The EANM and SNMMI practice guideline for lymphoscintigraphy and sentinel node localization in breast cancer. Eur J Nucl Med Mol Imaging. 2013;40(12):1932–47.

Giammarile F1, Alazraki N, Aarsvold JN, Audisio RA, Glass E, Grant SF, et al. Response to comment by Aprile et al.: the EANM and SNMMI practice guideline for lymphoscintigraphy and sentinel node

localization in breast cancer. Eur J Nucl Med Mol Imaging. 2014;41(6):1259–60.

Preparasi Radiofarmaka Nanokoloid....(Yunilda, dkk) 25

Misra PK, Dash U, Maharana S. Investigation of bovine serum albumin-surfactant aggregation and its physicochemical characteristics. Colloids Surfaces A Physicochem Eng Asp [Internet]. Elsevier B.V.; 2015;483:36–44. Available from: 2015.06.052

Amin S, Barnett G V., Pathak JA, Roberts CJ, Sarangapani PS. Protein aggregation, particle

formation, characterization& rheology. Curr Opin Colloid Interface Sci [Internet]. Elsevier B.V.; 2014;19(5):438–49. Available from:

Roberts CJ. Protein aggregation and its impact on product quality. Curr Opin Biotechnol Internet]. Elsevier Ltd;2014;30:211–7. Available from:

Zhanga S,Chena X, Ding S, Lei Q. Unfolding of human serum albumin by gemini and single-chain surfactants: A comparative study. Colloids Surfaces A Physicochem Eng Asp. 2016;495:30–8.

International Atomic Energy Agency. Radiopharmaceuticals for Sentinal Lymph Node Detection: Status and Trends. IAEA Hum Heal Ser. (6).Vienna:International Atomic Energy Agency;2015. Available


International Atomic Energy Agency. Technetium-99m radiopharmaceuticals: Manufacture of kits. Technical report series No. 466. Viena: Austria; 2008.

Santos I, Fernandes C, Maria L, Gano L, Santos IC, Paulo A. Re(I) and 99mTc(I) tricarbonyl complexes with ether-containing pyrazolyl-based chelators: Chemistry, biodistribution and metabolism We dedicate this paper to Professor Maria José Calhorda on the occasion of her 65th birthday. J Organomet Chem [Internet]. Elsevier B.V;2014;760:138–48. Available from: http://dx.

Motaleb MA, Lashine EM. Preparation and biological evaluation of 99m tc-tmpp as a novel agent for tumor diagnosis. Int J Adv Res. 2015;3(10):362–70.

Kluba CA, Mindt TL. Clickk-to-chelate: Development of technetium and rheniumtricarbonyl labeled radiopharmaceuticals. Molecules. 2013;18(3):3206–26.

Nurlaila Z. Formulasi radiofarmaka 99mtc-glutation untuk diagnosis kanker. J Sains dan Teknol Nukl Indones.2010;(2):77–86.

N, Triaspolitica. "Mengenal Penyakit Kanker, Jenis, Gejala, Penyebab Berikut Pengobatan Kanker." Mau Nanya Dong Dok. N.p, 20 June 2017. Web. 28 June 2017.>.

How to Cite
Yunilda Y, Awaludin R. Preparasi Radiofarmaka Nanokoloid Human Serum Albumin (HSA) untuk Limfosintigrafi. jki [Internet]. 28Feb.2017 [cited 29May2022];7(1):19-5. Available from: