Quantitative Analysis of Bioactive Compounds in Extract and Fraction of Star Fruit (Averrhoa carambola L.) Leaves using High Performance Liquid Chromatography

  • Nanang Yunarto Center for Biomedical and Basic Technology of Health, National Institute of Health Research and Development, Ministry of Health Republic of Indonesia
  • Novi Sulistyaningrum Center for Biomedical and Basic Technology of Health, National Institute of Health Research and Development, Ministry of Health Republic of Indonesia
Keywords: Apigenin, Quercetin, Averrhoa carambola L, HPLC

Abstract

Star fruit (Averrhoa carambola L.) is potential as raw material for medicine, native in tropic areas, including Indonesia. According to other study report, star fruit leaves containing flavonoids apigenin and quercetin as potential anti-inflammatory and anticancer agents. The raw material for the drug in Indonesia mostly obtained through imports from other countries. In order to support the independence of traditional medicine raw materials, it is important to standardize the quality of traditional medicine raw materials, in this case is star fruit leaves by High Performance Liquid Chromatography (HPLC) method. The sample used is star fruit leaves extract obtained from maceration process using ethanol 70%; water fraction, ethyl acetate and hexane fractions obtained from fractionation process of the ethanolic extract. Physical parameters analyzed in sample include appearance, color, odor, taste, extract yield, water content, loss of drying, total ash content, residual solvent. Chemical parameters analyzed include apigenin and quercetin contents. The results shows that star fruit leaves used in this study meet the standards of Indonesian Herbal Pharmacopoeia with highest apigenin (6.37%) and quercetin (4.49%) content are in ethyl acetate fraction.

References

Moresco HH, Queiroz GS, Pizzolatti MG, Brighente IMC. Chemical constituents and evaluation of the toxic and antioxidant activities of Averrhoa carambola leaves. Rev Bras Farmacog. 2012;22(2):319-24.

Khanam Z, Sam KH, Hazerra N, Chua HCI, Bhat UI. Determination of polyphenolic content, HPLC analyses and DNA cleavage activity of Malaysian A. carambola L. fruit extract. Journal of King Saud

University-Science. 2015;27(2):331-37.

Araho, D, Miyokoshi M, Chou WH, Kambara T, Mizutani KT. A New flavone C-glycoside from the leaves of Averrhoa carambola. Natural Medicine. 2005;59(3):113 -16.

Cabrini DA, Moresco HH, Imazu P, Delai da Silva C, Pietrovski EF, Gasparin DA. Analysis of the potential topical anti-inflammatory activity of Averrhoa carambola L. in mice. Evidence-Based

Complementary and Alternative Medicine. 2011;12:1-7.

Artanti N, Ma’arifa Y, Hanafi M. Isolation and identification of active antioxidant compound from star fruit (Averrhoa carambola) mistletoe (Dendrophthoe pentandra (L.) Miq.) ethanol extract. Journal

of Applied Science. 2006:6(1);1659-63.

Ministry of Health, Republic of Indonesia. Indonesia Herbal pharmacopeia. Jakarta. 2008.

Yunarto N, Elya B, Konadi L. Potency of ethyl acetate fraction of gambir leaves extract (Uncaria gambir Roxb.) as antihyperlipidemia. Jurnal Kefarmasian Indonesia. 2015;5(1):1-10.

Yunarto N, Aini N. Effect of Purified Gambir Leaves Extract to Prevent Atherosclerosis in Rats. Health Science Journal of Indonesia. 2015;6(2):105-10.

Dighe V, Mestry D, Shambhu N. High performance liquid chromatographic method Jurnal Kefarmasian Indonesia. 2017;7(1):26-33 for quantization of apigenin from dried root

powder of Gmelina arborea Linn. International Journal of Pharma and Biosciences. 2011;2(1):742-49.

Gulati G, Baghel S. Quantitative of quercetin from alcoholic extract Emblicao ficinalis by HPLC method. International Journal of Green Chemistry and Bioprocess. 2013;3(1):1-6.

Ministry of Health, Republic of Indonesia. Penetapan Parameter Standar Ekstrak. Jakarta. 2000.

World Health Organization. Quality control methods for herbal materials. 2nd Edition. Geneva. The Organization. 2011.

The United States Pharmacopeial Convention. The United States Pharmacopeia-National Formulary. 34th ed. Maryland: The United States Pharmacopeia Convention; 2011.

Chen XQ, Xiao JB. RP-HPLC-DAD detrmination of flavonoids: separation of quercetin, luteolin and apigenin in Marchantia convoluta. Iranian Journal of Pharmaceutical Research. 2010 Nov 20:175-81.

Moresco HH, Queiroz GS, Pizzolati MG, Brighente IMC. Chemical constituents and evaluation of the toxic and antioxidant activities of Averrhoa carambola leaves. Revista Brasileira de armacognosia.

;22(2):319-24.

Funakoshi-Tago M, Nakamura K, Tago K, Mashino T, Kasahara T. Anti-inflammatory activity of structurally related flavonoids, Apigenin, Luteolin and Fisetin. International Immuno Pharmacology. 2011;11(9):1150-9.

Baghel SS, Shrivastava N, Baghel RS, Agrawal P, Rajput S. A review of quercetin: antioxidant and anticancer properties. World J Pharm Pharmaceutical Sci. 2012 May 1;1(1):146-60.

Dajas F. Life or death: neuroprotective and anticancer effects of quercetin. Journal of ethnopharmacology. 2012 ;143(2):383-96.

Published
2017-02-28
How to Cite
1.
Yunarto N, Sulistyaningrum N. Quantitative Analysis of Bioactive Compounds in Extract and Fraction of Star Fruit (Averrhoa carambola L.) Leaves using High Performance Liquid Chromatography. jki [Internet]. 28Feb.2017 [cited 26May2022];7(1):26-3. Available from: https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/3481
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