Isolasi, Identifikasi, Karakterisasi, dan Uji antibiofilm Derivat Asam Galat dari Kulit Batang Sterculia quadrifida R.Br

  • Rollando Rollando Jurusan Farmasi, Fakultas Sains dan Teknologi, Universitas Ma Chung, Malang, Indonesia
Keywords: Faloak, Isolate, Gallic acid derivative, Antibiofilm

Abstract

Faloak (Sterculia quadrifida R.Br) is used empirically by residents of Timor island to treat hepatitis, typhoid, ulcers, and to restore stamina. Information of active compound contained in the bark of faloak specifically unpublished. This study aims to determine the active compound contained in faloak bark that can be used as antibiofilm. The extraction was performed by soxletation method, the isolation was performed by gradien isolation method, the elusidation was performed by merging information from NMR and LC-MS spectra analysis. The biofilm inhibition activity test was performed by microdilution method formed on flat bottom flexible microplate U-bottom PVC 96 wells with staining using 1% crystal violet. The isolation process obtained 3 gallic acid derivatives, named isolate 1, isolate 2, and isolate 3. Test of inhibition of biofilm formation showed isolate 1 has IC50 of 46,87 µg/mL, isolate 2 has IC50 of 45,87 µg/mL, and isolate 3 has IC50 42,65 µg/mL.The biofilm test results showed that isolates 1-3 had high biofilm inhibition potency.

References

Siswadi, Rianawati H, Saragih G, Hadi, D. The potency of faloak's (Sterculia quadrifidaR.Br ) active compunds as natural remedy. Prosiding Seminar International, Kementrian Kehutanan bagian Penelitian dan Pengembangan Hutan, Bogor. 2013;4-10.

Fabianus R. Aktivitas Anticendawan Zat Ekstraktif Faloak (Sterculia comosa Wallich). Jurnal Kehutanan IPB. 2012;67(2):6-11.

Rollando. Penelusuran Potensi Aktivitas Antibakteri dan Antioksidan Fraksi Kulit Pohon Faloak (Sterculia quadrifida R.Br). Jurnal Kefarmasian. 2016;4:1-5.

Shakh MAR, Ohara-Nemoto T, Ono Y, Shimoyama S, Kimura TK, Nemoto K. In vitro processing of glutamyl endopeptidaseproenzymes from Entero- coccus faecalisand importance of N-terminal residue in enzyme catalysis, Advances in Biochemistry. 2013; 1(5): 73-80.

Darby ML, Margaret MW. Dental hygiene: theory and practice, 3rd ed. Elsevier, London. 2010. H. 3,16

Madigan MT, Martinko JM, Brock TD. Brock. Biology of Microorganisms. 14th Ed. New Jersey: Pearson Prentice. 2014;617-619.

Eke PI, Dye BA, Wei L, Thornton-Evans GO, Genco RJ. Prevalence of periodontitis in adults in the United States. Dent Res. 2010;91(10):914-20.

Arinda A, Rahardjo P, Triwardani A., Perbedaan efektivitas obat kumur yang mengandung cengkeh dengan obat kumur chlorexidine gluconat 0.2% dalam menghambat pembentukan plak. Orthodontic Dental Journal. 2012;01:22-25.

Raina L. Ensiklopedi tumbuhan berhasiat obat. Jakarta: Salemba Medika. 2011;13-19.

Daglia, M. Polyphenols as antimicrobial agents. Curr. Opin. Biotechnol. 2012;23:174–181

Quideau S, Deffieux D, Douat-Casassus C, Pouysegu, L.Plant polyphenols: Chemical properties, biological activities, and synthesis. Angew. Chem. Int. Ed. Engl. 2011;50:586–621.

Cushnie TP, Lamb AJ.Recent advances in understanding the antibacterial properties of flavonoids. Int. J. Antimicrob. Agents. 2011;38:99–107.

Ren Z, Chen L, Li J, Li Y. Inhibition of Streptococcus mutans polysaccharide synthesis by molecules targeting

glycosyltransferase activity. J. Oral Microbiol. 2016;8:31-95.

Kang, MS, Oh JS, Kang IC, Hong SJ, Choi CH. Inhibitory effect of methyl gallate and gallic acid on oral bacteria. J. Microbiol. 2008;46:744–750.

Krzy´sciak W, Jurczak A, Ko´scielniak D, Bystrowska B, Skalniak A. The virulence of Streptococcus mutans and the ability to form biofilms. Eur. J. Clin. Microbiol. Infect. 2014;33:499–515.

Published
2017-08-22
How to Cite
1.
Rollando R. Isolasi, Identifikasi, Karakterisasi, dan Uji antibiofilm Derivat Asam Galat dari Kulit Batang Sterculia quadrifida R.Br. jki [Internet]. 22Aug.2017 [cited 20May2022];7(2):105-11. Available from: https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/3497
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