Konsentrasi Aman Kurkumin dan PGV-0 terhadap Sel Vero Berdasarkan Hasil Uji Sitotoksik

Dewi Marbawati; Sarjiman Sarjiman

doi:10.22435/jki.v5i2.3687

Dewi Marbawati Balai Litbang Pengendalian Penyakit Bersumber Binatang, Banjarnegara, Jawa Tengah
Sarjiman Sarjiman Fakultas Farmasi, Universitas Gadjah Mada, Sekip Utara, Yogyakarta

DOI: https://doi.org/10.22435/jki.v5i2.3687

Keywords: Curcumin, Pentagamavunon-0, Cytotoxic test, Vero cell

Abstract

Curcumin (1,7-bis(3-methoxyphenyl 4'hidroksi) -1.6 heptadien, 3,5-dione) is the yellow pigment of Curcuma longa. Curcumin has various biological activities such as antioxidant, antibacterial, antifungal, antiprotozoal and antivirus. Various benefits of curcumin can not be separated from the weakness which is not stable to pH and light. Pentagamavunon-0 (PGV-0) were made by changing the β diketone group on cluster analog of curcumin into monoketon while eliminating active methylene group so it is more stable to pH and light. PGV-0 also has potential as a stronger antioxidant and antiinflammatory agent than other curcumin analogues. The objective of this research was to determine the safe concentrations of curcumin and PGV-0 on vero cells due to the increased use of the two compounds through the cytotoxic test. This research includes experimental research. Cytotoxic test performed with microculture tetrazolium technique (MTT) method. Use of MTT to evaluate the cytotoxic is based on changes of tetrazolium salt into formazan crystals by mitochondrial enzyme succinate dehydrogenase with the help of cellular NADH. The results showed that the safe concentrations of curcumin and PGV-0 on vero cells respectively are 6.25 and 1.5625 ppm. Based on the cytotoxic test the secure concentration of curcumin was higher than PGV-0.

References

Gupta SC, Patchva S, Aggarwal BB. Therapeutic roles of curcumin: lessons learned from clinical trials. AAPS J. 2013;15(1):195–218.

Singh RK, Rai D, Yadav D, Bhargav A, Balzarini J, De Clercq E, et al. Synthesis, antibacterial and antiviral properties of curcumin bioconjugates bearing dipep-tide, fatty acids and folic acid, European Journal of Medicinal Chemistry. 2010; 45: 1078–86.

Hatcher H, Planalp R, Cho J, Torti FM, Torti SV. Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci. 2008;65(11):1631-52.

Chattopadhyay I, Biswas K, Bandyopadhyay U and Banerjee RK, Turmeric and Curcumin: Biological Actions and Medicinal Applications. Current Science. 2004; 87(1):44-53.

Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin: the Indian solid gold. Adv Exp Med Biol. 2007;595:1-75.

Buadonpri W, Wichitnithad W, Rojsitthisak P, Towiwat P. Synthetic cucumin inhibits carragennan-induced paw edema in rats. J Health Res. 2009; 23(1):11- 6.

Singh RK, Rai D, Yadav D, Bhargava A, Balzarini J, De Clercq E. Synthesis, antibacterial and antiviral properties of curcumin bioconjugates bearing dipeptide, fatty acids and folic acid. European Journal of Medicinal Chemistry. 2010;45:1078–86

Gangait A, Barman T, Mukherjee PK. Validated method for the estimation of curcumin in turmeric powder. Indian journal of traditional knowledge. 2011;10(2):247-50.

Tonnesen HH, Solubility, chemical and photochemical stability of curcumin in surfactant solutions. Studies of curcumin and curcuminoids, XXVIII, Pharmazie. 2002;57(12):820-4.

Liang G, Shao L, Wang Y, Zhao C, Chu Y, Xiao J. Exploration and synthesis of curcumin analogues with improved structural stability both in vitro and in vivo as cytotoxic agents. Bioorganic and Medical Chemistry. 2009;17(6):2623-31.

Kumavat SD, Chaudhari YS, Borole P, Mishra P, Shenghani K, Duvvuri P. Degradation Studies Of Curcumin. International Journal of Pharmacy Review & Research. 2013;3(2),50-5.

Sharma RA, Gescher AJ, Steward WP. Curcumin: The story so far. Eur. J. of Cancer. 2005;41:1955- 68.

Van der Goot H. The chemistry and qualitative structure-activity relationship of curcumin, in Recent Development in Curcumin Pharmacochemistry, Proce-dings of The International Symposium on curcumin Pharmacochemistry (ISCP), August 29 – 31. 1995. Edited by Suwijiyo Pramono, Aditya Media, Yogyakarta. Indonesia.1997

Sardjiman. Synthesis of Some New Series of Curcumin Analogues, Anti-oxidative, anti-inflamatory, antibacterial activities and qualitative-structure Activity Relationship. Dissertation. Gadjah Mada University. Yogyakarta; 2000.

Yuniarti N, Nugroho PA, Asyhar A, Sardjiman S, Ikawati Z, Istyastono EP. In vitro and in silico studies on curcumin and its analogues as dual inhibitors for cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2). Journal of Mathematic and Fundamental Science. 2012;44(1):51-66.

Handler N, JaegerW, Puschacher H, Leisser K, Erker T. Synthesis of novel curcumin analogues and their evaluation as selective cyclooxygenase-1 (COX-1)iInhibitors, Chem. Pharm. Bull. 2007;55:64-71.

Sons WJ. 2008. vero cell. Curr protocol Microbiology. Inggris.

Doyle A and JB Griffits. Cell and Tissue Culture for Medical Research. New York: John Wiley & Sons. 2000.

Rachmayanti H. Uji Sitotoksik Fraksi Non Polar Ekstrak Etanol Kulit Kayu Srikaya (Annona squamosa L) Terhadap Sel T47D. Skripsi. Fakultas Farmasi Universitas Muhammadiyah Surakarta

Nogaki A, Satoh K, Iwasaka K, Takano H, Takahama M, Ida Y and Sakagami H. Radical intensity and cytotoxic activity of

curcumin and gallic acid, Anticancer Research. 1998;18:(5A). 3487-91.

Zandia K, Ramedani E, Mohammadi K, Tajbakhsh S, Deilami I, Rastian Z, et.al.. Evaluation of Antiviral Activities of Curcumin Derivatives against HSV-1 in Vero Cell Line. Natural Product Communications (NPC). 2010; 5(12): 1935 – 38.