Kajian Potensi Penggunaan Bioreaktor terhadap Senyawa Ajmalisin Suatu Contoh Produksi Metabolit Sekunder Tanaman Obat

  • Ratih Dian Saraswati Pusat Biomedis dan Teknologi Dasar Kesehatan, Badan Litbangkes Kemenkes RI
Keywords: Bioreactor, Ajmalicine, C. roseus

Abstract

The limited availability of medicinal plants as well as their limitations in cultivation as a source of medicinal compounds can lead to excessive harvesting, habitat destruction and destructive exploration. This led to the extinction of rare plants and caused the condition of natural resources worse. The purpose of this study is to elucidate the potential of bioreactor in terms of its ability to increase the active compounds of plant obtained from C. roseus ajmalicine. The data was obtained from various references. The main references discuss about ajmalicine and bioreactor.In general, the concentration of compounds produced by cell suspension is higher than its original concentration in the plants. In study cases of ajmalicine, the compounds were produced as much as seven times more than these compounds in native plants. Other studies show that the use of different plant organs, the addition of elicitor, the weight ofinitial cells as well as aeration rate can affect the concentration of ajmalicine obtained from medium in the bioreactor. Tissue cultured plants in bioreactor can produce secondary metabolites in a short time with high yields. However, previous optimazation should be carried out in terms of adding elicitor, aeration rate and pH condition of the bioreactor

References

1.Kardono, L.B. Sugeng., Pe- ngembangan Bahan Baku Obat Berbasis Sumber Daya Hayati. Orasi Pengukuhan Profesor Riset Bidang Kimia Organik. LIPI. Jakarta. 2009
2.Isnawati, Ani. Pengembangan Metoda Isolasi Untuk Mendapatkan Kadar Artemisinin Yang Optimal Guna Menuju Kemandirian Bahan Baku Obat Malaria. Laporan Akhir Penelitian. Pusat Penelitian dan Pengembangan Biomedis dan Farmasi Badan Litbangkes. Jakarta.2006
3.WHO. 2008. Traditional Medicine. Fact sheetNo.134http://www.who.int/mediacentre/factsheets/fs134/en/. Diakses tanggal 5 Mei 2011
4.Dodds, John H., Lorin W. Roberts., Experiment in Plant Tissue Culture. 2nd Ed. Cambridge University Press. Cambridge. 1985
5.Macaskill, Craig., The National Agricultural Directory 2011. RainbowSA.
6.http://www.weizmann.ac.il/plants/aharoni/PlantMetabolomeCourse/May022007.pdf
7.Schultz, Jack. Secondary Metabolites inPlantshttp://www.biologyreference.com/Re-Se/Secondary-Metabolites-in-Plants.html
8.Indrayanto, Gunawan. 2010. Prospek Kultur Jaringan Tanaman Untuk Produksi Kimia BahanAlam.http://garuda.dikti.go.id/jurnal/detil/id/2:11823/q/bioreaktor%20tanaman%20obat/offset/0/limit/3. Diakses tanggal 5 Mei 2011.
9.Mukarlina, R.R. Esyanti, Arbayah H. Siregar. Pengaruh Pemberian Elisitor Homogenat Jamur Pythium aphanidermatum (Edson) Fitzp. Terhadap Kandungan Ajmalisin dalam Kultur Akar Catharanthus roseus (L.) G. Don. Jurnal Matematika dan Sains, 2006,Volume 11, No.2, 44-49
10.Radji, Maksum. Peranan Bioteknologi dan Mikroba Endofit dalam Pen gembangan Obat Herbal. Majalah Ilmu Kefarmasian, 2005, Volume 2, No.3, 113-126
11.Revolusi Bioteknologi, Ed. Jean L. Marx. Yayasan Obor Indonesia. Jakarta. 1991
12. Hay. Diaplikasikan di Pelbagai Bidang Kehidupan. Koran Jakarta, http://www.ristek.go.id/file/upload/Referensi/2010/digital_cliping/Klipping%20Berita20Iptek%2013-11-2010.pdf. Hal 10/22. Diakses tanggal 5 Mei 2011
13. Gupta, Yanka., Selected Readings in Microbiology and Genetics. Sarup & Sons. 1998
14. Subroto, M. Ahkam, Nina Artanti. Produksi Metabolit Sekunder dari Tanaman Melalui Teknik Biak Sel dan Jaringan : Suatu Rintisan Ke Arah Tahap Komersialisasi Industri. Warta Biotek, 1996, Volume 10, No. 1, 5-10.
15. Esyanti, R.R., Aida Muspiah. Pola Produksi Ajmalisin dari Kultur Agregat Sel catharanthus roseus (L.) G Don. dalam Bioreaktor Airlift. Hayati 2006, Volume 13, No.4, 161-165
16. Kumar, Padma, 2010. Plant Bioreactors Types - Their Advantages and Disadvantages http://www.biotecharticles.com/OthersArticle/Plnt-Bioreactors-Types-Their-Advantages-and-Disadvantages-259.html. Diakses tanggal 5 Mei 2011
17. Misawa, Manasaru. Plant Tissue Culture : An Alternative For Production of Useful Metabolites. Food & Agriculture Org. 1994.
Published
2012-02-24
How to Cite
1.
Saraswati R. Kajian Potensi Penggunaan Bioreaktor terhadap Senyawa Ajmalisin Suatu Contoh Produksi Metabolit Sekunder Tanaman Obat. jki [Internet]. 24Feb.2012 [cited 20May2022];2(1):28-4. Available from: https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/3714
Section
Articles