Antimicrobial Activity of Fractions of Ethyl Acetate Extract of Cladosporium oxysporum, An Endophytic Fungus Derived from Alyxia reinwardtii

  • Dwi Hartanti Faculty of Pharmacy, University of Muhammadiyah Purwokerto, Banyumas, Central Java, Indonesia
  • July Supriyanto Faculty of Pharmacy, Airlangga University, Surabaya, East Java, Indonesia
  • Noor Erma N Sugijanto Faculty of Pharmacy, Airlangga University, Surabaya, East Java, Indonesia
Keywords: Alyxia reinwardtii, Antimicrobial, Cladosporium oxysporum, endophytic fungi


The endophytic fungi Cladosporium oxysporum was isolated from medicinal plants Alyxia reinwardtii. The antimicrobial activity of fractions of ethyl acetate extract and also antioxidant and antimicrobial activities of fraction 7 of ethyl acetate extract of (AR-7) were determined in this study. Antimicrobial activity was determined by TLC bioautography and disc diffusion methods. Antioxidant activity was determined by TLC DPPH autography assay. The fractions of ethyl acetate extract of C. oxysporum at dose of 100 mg/ml were active as antimicrobial against S. aureus, E. coli and C. albicans in disc diffusion method test. Fraction AR-7 was active as antioxidant in TLC-DPPH autography assay. Fraction AR-7 was active as antimicrobial against S. aureus, but was not active against another tested microoorganisms in TLC-bioautography


1. Schulz B, Boyle C. What are endophytes? In: Schulz B, Boyle C, Sieber TN, editors. Soil Biology: Microbial Root Endophytes. Berlin Springer-Verlag; 2006. p. 1-13.
2. Alvin A, Miller KI, Neilan BA. Exploring the potential of endophytes from medicinal plants as sources of antimycobacterial compounds. Microbiology Research. 2014;169:483–95.
3. Radic N, Strukelj B. Endophytic fungi - The treasure chest of antibacterial substances. Phytomedicine Journal. 2012;19:1270-84.
4. Suryanarayanan TS. Endophyte research: going beyond isolation and metabolite documentation. Fungal Ecology.
5. Mangestuti, Subehan, Widyawaruyanti A, Zaidi SFH, Awale S, Kadota S. Traditional medicine of Madura island in Indonesia. Journal of Traditional Medicine. 2007;24:90-103.
6. Pribadi ER. Pasokan dan permintaan tanaman obat Indonesia serta arah penelitian dan pengembangannya. Perspektif. 2009;8(1):52-64
7. Kitagawa I, Shibuya H, Baek NI, Yokokawa Y, Nitta A, Wiriadinata H, et al. Pulosarioside, a new bitter trimericiridoid diglucoside, from an Indonesian
jamu, the bark of Alyxia reinwardtii BL. (Apocynaceae). Chemistry and Pharmaceutical Bulletin. 1988;36(10):4232-5.
8. Rattanapan J, Sichaem J, Tippyang S. Chemical constituents and antioxidant activity from the stems of Alyxia reinwardtii. Record of Natural Product.
9. Steffan B, Watjen W, Michels G, Niering P, Wray V, Ebel R, et al. Polyphenols from plants used in traditional Indonesian medicine (Jamu): uptake and antioxidative effects in rat H4IIE hepatoma cells. Journal of Pharmacy and Pharmacology. 2005;57:233–40.
10. Topcu G, Che CT, Cordell GA, Ruangrungsi N. Iridolactones from Alyxia reinwardtii. Phytochemistry. 1990;29(10):3197-9.
11. Strobel G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiology and Molecular Biology Review. 2003;67(4):491–502.
12. Sugijanto NE, Diesel A, Ebel R, Indrayanto G, Zaini NC. Chemical constituents of the endophytic fungus Lecythophora sp. isolated from Alyxia reinwardtii. Natural Product Communication. 2009;4(11):1485-8.
13. Sugijanto NE, Diesel A, Rateb M, Pretsch A, Gogalic S, Zaini NC, et al. Lecythomycin, a new macrolactone glycoside from the endophytic fungus Lecythophora sp. Natural Product Communication. 2011;6(5):677-8.
14. Huang WY, Cai YZ, Hyde KD, Corke H, Sun M. Endophytic fungi from Nerium oleander L (Apocynaceae): main constituents and antioxidant activity.
World Journal of Microbiology and Biotechnology. 2007;23:1253-63.
15. Samaga PV, Rai VR. Free radical scavenging activity and active metabolite profiling of endophytic fungi from Nothapodytes foetida and Hypericum
mysorense. International Journal of Chemistry and Analitical Sciences. 2013;4:96-101.
16. Samaga PV, Rai VR, Rai KML. Bionectria ochroleuca NOTL33 - an endophytic fungus from Nothapodytes foetida producing antimicrobial and free radical
scavenging metabolites. Annals of Microbiology. 2014;64:275-85.
17. Song YC, Huang WY, Sun C, Wang FW, Tan RX. Characterization of graphis lactone A as the antioxidant and free radical-scavenging substance from the culture of Cephalosporium sp. IFB-E001, an endophytic fungus in Trachelospermum jasminoides. Biological and Pharmaceutical Bulletin. 2005;28(3):506-9.
18. Zhao J, Zhang JS, Yang B, Li GP, Li SP. Free radical scavenging activity and characterization of sesquiterpenoids in four species of Curcuma using a TLC bioautography assay and GC-MS Analysis. Molecules. 2010;15:7547-57.
19. Ho MY, Chung WC, Huang HC, Chung WH, Chung WH. Identification of endophytic fungi of medicinal herbs of Lauraceae and Rutaceae with antimicrobial property. Taiwania. 2012;57(3): 229-41.
20. Zheng L, Chen H, Han X, Lin W, Yan X. Antimicrobial screening and active compound isolation from marine bacterium NJ6-3-1 associated with the sponge Hymeniacidon perleve. World Journal of Microbiology and Biotechnology. 2005;21:201-6.
21. Paul NC, Yu SH. Two species of endophytic Cladosporium in Pine trees in Korea. Mycobiology. 2008;36(4):211-6.
22. Bensaci OA, Daoud H, Lombarkia N, Rouabah K. Formulation of the endophytic fungus Cladosporium oxysporum Berk. & M.A. Curtis, isolated from Euphorbia
bupleuroides subsp. luteola, as a new biocontrol tool against the black bean aphid (Aphis fabae Scop.). Journal of Plant Protection Research. 2015;55(1):80-7.
23. Sholihah NI. Aktivitas antimikroba ekstrak metanol, etil asetat,dan diklorometana jamur endofit Cladosporium oxysporum (ALE C) dari Alyxia reinwardtii BL. .
Surabaya: Airlangga University; 2008.
24. Ciesla Ł, Kryszen J, Stochmal A, Oleszek W, Waksmundzka-Hajnos M. Approach to develop a standardized TLC-DPPH• test for assessing free radical scavenging
properties of selected phenolic compounds. Journal of Pharmaceutical and Biomedical Analysis. 2012;70:126-35.
25. Hamid AA, Aiyelaagbe OO, Usman LA, Ameen OM, Lawal A. Antioxidants: Its medicinal and pharmacological applications. African Journal of Pure and Applied Chemistry. 2010;4(8):142 -51.
26. Choma IM, Grzelak EM. Bioautography detection in thin-layer chromatography. Journal of Chromatography A. 2011;1218:2684–91.
27. Jadulco R, Brauers G, Edrada RA, Ebel R, Wray V, Sudarsono, et al. New metabolites from sponge-derived fungi Curvularia lunata and Cladosporium herbarum. Journal of Natural Product. 2002;65:730-3.
28. Shigemori H, Kasai Y, Komatsu K, Tsuda M, Mikami Y, Kobayashi Ji. Sporiolides A and B, new cytotoxic twelve-membered macrolides from a marine-derived fungus Cladosporium species. Marine Drugs. 2004;2:164-9.
29. Wang FW. Bioactive metabolites from Guignardia sp., an endophytic fungus residing in Undaria pinnatifida. Chinese Journal of Natural Medicines. 2012;10(1):0072-6.
How to Cite
Hartanti D, Supriyanto J, Sugijanto N. Antimicrobial Activity of Fractions of Ethyl Acetate Extract of Cladosporium oxysporum, An Endophytic Fungus Derived from Alyxia reinwardtii. jki [Internet]. 29Feb.2016 [cited 20May2022];6(1):1-. Available from: