Jurnal Kefarmasian Indonesia https://ejournal2.litbang.kemkes.go.id/index.php/jki <p>ISSN media elektronik:&nbsp;<a href="http://u.lipi.go.id/1387251248">2354-8770</a></p> <p>ISSN media cetak:&nbsp;<a href="http://u.lipi.go.id/1329660553">2085-675X</a></p> <p>Jurnal Kefarmasian Indonesia is a scientific journal published by Center for Research and Development of Biomedical and Basic Health Technology, Board of Health Research and Development, Ministry of Health Republic of Indonesia (Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan, Badan Penelitian dan Pengembangan Kesehatan&nbsp;Kementerian Kesehatan Republik Indonesia). The journal publishes original research articles in pharmaceutical science such as Pharmaceutical Technology, Pharmacology, Pharmaceutical Chemistry, Traditional Medicines and Pharmaceutical Care. The journal was established in 2009 while online publication has been started from 2015. The journal is published in Bahasa Indonesia and English. Jurnal Kefarmasian Indonesia is a biannual, open access, peer-reviewed and online pharmacy journal. Jurnal Kefarmasian Indonesia aims to serve the updated scientific knowledge for processing manuscript as well as for processing manuscript. The journal has been acredited by Ristekdikti Accreditation 21/E/KPT/2018 and Indonesian Institute of Science by Decree No. 783/AU1/P2MI-LIPI/11/2017.</p> <p>Jurnal Kefarmasian Indonesia has been indexed by <a href="https://doaj.org/toc/2354-8770?source=%7B%22query%22%3A%7B%22filtered%22%3A%7B%22filter%22%3A%7B%22bool%22%3A%7B%22must%22%3A%5B%7B%22term%22%3A%7B%22index.issn.exact%22%3A%222085-675X%22%7D%7D%2C%7B%22term%22%3A%7B%22index.issn.exact%22%3A%222354-8770%22%7D%7D%2C%7B%22term%22%3A%7B%22_type%22%3A%22article%22%7D%7D%5D%7D%7D%2C%22query%22%3A%7B%22match_all%22%3A%7B%7D%7D%7D%7D%2C%22from%22%3A0%2C%22size%22%3A100%7D">DOAJ</a>; <a href="http://isjd.pdii.lipi.go.id/index.php/public_no_login/index_direktori">Indonesian Scientific Journal Database</a>, <a href="http://id.portalgaruda.org/?ref=browse&amp;mod=viewjournal&amp;journal=4889">Indonesian Publication Index</a>, <a href="https://www.base-search.net/Record/1497b5f9cf595ef0ff1193d5afab7b2f432efcff10643f42eacaf41a5aa3aa83/">Bielefeld Academic Search Engine</a>, <a href="http://oaji.net/journal-detail.html?number=825">Open Academic Journal Index</a>; <a href="https://scholar.google.co.id/citations?user=gASqT4MAAAAJ&amp;hl=en">Google Scholar</a>; <a title="Sinta" href="http://sinta2.ristekdikti.go.id/journals/detail?id=2785">Sinta</a> Garda Rujukan Digital (<a href="http://garuda.ristekdikti.go.id/journal/view/4889">Garuda</a>). See Google Scholar Profile for Jurnal Kefarmasian Indonesia by clicking <a href="https://scholar.google.co.id/citations?user=gASqT4MAAAAJ&amp;hl=en">here</a>.</p> <p>Total citations: 193</p> <p>Total documents: 94</p> <p>h-index: 7&nbsp; &nbsp; &nbsp;i10-index: 3&nbsp; &nbsp; &nbsp;Impact Factor: 2,06</p> Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan en-US Jurnal Kefarmasian Indonesia 2085-675X Front Matter Volume 9 No 1 Tahun 2019 https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/1817 Editor JKI ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-17 2019-05-17 Efek Sinergis Kombinasi Ekstrak Etanolik Kayu Secang dan Rimpang Lempuyang pada Sel Kanker Payudara MCF-7 https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/324 Cancer is one of critical, chronic, and complex disease, also becoming the high cause of death in the world. Brazilin and brazilein in sappan wood (Caesalpinia sappan L.) and zerumbone in bitter ginger rhizome (Zingiber zerumbet L.) are known having cytotoxic activity with different mechanisms. This study aimed to examine combination effect of sappan wood and bitter ginger rhizome. Sappan wood and bitter ginger rhizomes macerated with ethanol 96% for 3x24 hours, filtered, and evaporated to obtain dried extract.Cytotoxic effect on MCF-7 cells was done using MTT assay. Combination Index (CI) was determined by CompuSyn based on the result of cytotoxic combination. Cell cycle profile and apoptosis induction was analyzed by flow cytometry. Sappan wood extracts and bitter ginger rhizome exhibited cytotoxic effects on MCF-7 cells with the IC50 values of 30 and 155 μg/mL respectively. The combination of sappan wood 15 μg/mL and bitter ginger 8, 12, 24, and 60 μg/mL produced synergistic effect with the CI value of 0.57-0.85. Sappan wood 15 μg/mL combined with bitter ginger 8 and 24 μg/mL showed cell cycle inhibition at G2/M phase. The combination also increased apoptosis induction compared to untreated cells and its single treatment.The combination of sappan wood ethanolic extracts and bitter ginger rhizome showed synergistic cytotoxic effect. Its synergism effect revealed through cell cycle arrested at G2/M phase and acceleration of apoptotic induction. Sari Haryanti Ika Yanti M. Sholikhah Yuli Widiyastuti ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-16 2019-05-16 1 9 10.22435/jki.v9i1.324 Uji Aktivitas Antiseptik Ekstrak Etanol Daun Sirih (Piper betle Linn.) dalam Obat Kumur terhadap Staphylococcus aureus secara in Vitro https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/351 Betle leaf (Piper betle Linn.) is very popular in Indonesia. Betle leaf is useful for dental health and frequently used as mouthwash; to eliminate body and mouth odor; treats mouth ulcer, nosebleed, itching, ulceration and vaginal discharge in women. An oral cavity is a place for bacteria. If there is a bacterial immunity reduction, that was originally commensal bacteria can turn into a pathogen that cause infection. One of bacteria that commonly found in the oral cavity is Staphylococcus aureus. The bacteria have the ability to occupy and form biofilm on biomaterials that cause resistant to antimicrobials, thus making it difficult to eradicate host that is infected by S. aureus. Previous study reported a multidrug resistant of S. aureus that could cause death, that to overcome it, betle leaves extract ethanol in vitro antiseptic test to S.aureus was conducted. The test was conducted by the phenol coefficient method using S. auereus ATCC 25923 bacteria. Povidone iodine mouthwash that is circulated in the market is used as a comparison. The results showed that mouthwash containing ethanol extract of betle leaf had a phenol coefficient value of 1.87 while povidone iodine mouthwash as a comparison, had a phenol coefficient of 1.0. Mouthwash containing ethanol extract of betle leaf had higher antiseptic effectiveness than the comparative mouthwash. Dian Sundari Almasyhuri Almasyhuri ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-16 2019-05-16 10 18 10.22435/jki.v9i1.351 The Effect of Infusion of Syzygium polyanthum (Wight) Walp. Leaves as Natural Preservative Chicken Meats https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/1100 Syzygium polyanthum (Wight) Walp. (Indonesian bay leaf) is known for possessing antimicrobial activity that might be applied for natural food preservative. In this study, we analyzed the constituents of infusion of S. polyanthum leaves and evaluated its potency as the preservative of fresh chicken meats. The infusions were prepared with the method described in Indonesian Pharmacopeia. Phytochemical content of infusion of S. polyanthum leaves was analyzed by qualitative phytochemical screening using colorimetric methods. Its application for preservative of fresh chicken meat was evaluated based on its activity in inhibiting the growth of bacteria on the meats as well as the physical observation of the preserved meats. The infusion of S. polyanthum leaves contained flavonoids, tannins, and saponins. The infusion at the optimum concentration of 10% was capable of inhibiting bacterial growth on chicken meats and extending their shelf life up to 3 days in temperature of 3-7ºC. Dwi Hartanti Asmiyenti Djaliasrin Djalil Alwani Hamad Nur Yulianingsih ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-16 2019-05-16 19 27 10.22435/jki.v9i1.1100 Keragaman Bakteri Endofit Penghasil L-Asparaginase Bebas L-Glutaminase https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/1302 Endophytes are potential as a source of active compound producer. Endophytes that is isolated from tropical medicinal plants has been getting attention due to its high biodiversity and active compound producing ability. L-asparaginase is the first enzyme used as chemotherapeutic agent for leukemia. The aim of this study is to collect the endophytic bacteria associated with tropical medicinal plants from Sumba Island and investigate the activity of L-asparaginase without L-glutaminase from endophytic bacteria isolates. The samples Gliricidia sepium, Pittosporum moluccanum, Clerodendrum buchanani, and Zingiberaceae are collected from Wanggameti, East Sumba, NTT. Samples are sterilized using surface-sterilization method and endophytic bacteria are isolated using plant piece method on R2A media. Selected endophytic bacteria are identified by 16S rDNA sequences. L-asparaginase screening is conducted using modified R2A with addition of L-asparagine and phenol red as colour indicator. A total of 34 isolates of endophytic bacteria were collected from 5 samples. A total of 14 genus consisted of 17 different bacterial species were obtained from 34 selected isolates. Endophytic bacteria of P. stutzeri strains of SMKL1 and R. radiobacter strains of SMKW2 from the Kahili plant were needed as L-glutaminase-free L-asparaginase and were potential to be candidate of leukemia cancer chemotherapy tri ratna sulistiyani dinihari indah kusumawati ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-16 2019-05-16 28 39 10.22435/jki.v9i1.1302 Studi in Silico Lima Senyawa Aktif Sebagai Penghambat Protein Virus Dengue https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/1157 Dengue infection is an endemic disease in the tropics and subtropics, caused by dengue virus (DENV) infection. Some compounds have been shown to have antiviral effects on some viruses. In silico study is conducted to predict the stability of natural ingredient compounds: artemisinin, catechin, mangiferin, epigallocatechin gallate (EGCG), and quercetin in their interactions with dengue virus proteins at molecular level. This study is carried out using the 2008 version of the Molecular Operating Environment (MOE) software. Ligands are ribavirin as antiviral control whereas artemisinin, mangiferin, EGCG, and quercetin with 3D mole format structures. The downloaded DENV protein with PDB document format is the DENV serotype 2 envelope protein with 1OKE code, non structural protein 3 (NS3) with 2VBC code and NS5 protein with 1L9K code. In silico test generally showed that catechin, mangiferin, EGCG, and quercetin had more stable docking ligands to DENV’s proteins. In particular, mangiferin had stable docking ligand to envelope proteins, NS3 (helicase and protease) and in NS5-methyltransferase compared to ribavirin. Catechin stabled on NS3-protease, EGCG on NS3 (helicase and protease) and quercetin on NS3-protease. Artemisinin had less stabled bonds than ribavirin. The results indicated that catechin, mangiferin, EGCG, and quercetin had potential inhibition to DENV proteins whereas mangiferin was the most potential compound to inhibit dengue virus protein targets. Reni Herman ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-17 2019-05-17 40 47 10.22435/jki.v9i1.1157 Aktivitas Antibakteri Minyak Atsiri Daun Tembakau terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/1219 Tobacco plants are widely used as raw material for cigarettes, but the results of the study show that in tobacco leaves contain compounds that can be used in the pharmaceutical and health fields. Tobacco leaves contain essential oils that have a distinctive aroma. The study aims to examine the antibacterial activity of tobacco essential oil from six sources tobacco such as Temanggung, Yogyakarta, Purwodadi, Boyolali, Blitar and Probolinggo. Tobacco essential oil is obtained by the steam-water distillation method. Antibacterial activity evaluation was carried out by diffusion method and the minimum inhibitory concentration on two bacteria, Escherichia coli and Staphylococcus aureus. The yield of tobacco essential oil ranged from 0.54-3.39%. Tobacco essential oil had antibacterial activity against bacteria E. coli and S. aureus in the presence of inhibition zones in bacterial media. The biggest inhibition zone was in Yogyakarta tobacco essential oil of 26 mm for S. aureus bacteria, while for E. coli bacteria the biggest inhibition zone was Blitar tobacco essential oil which was equal to 21 mm. The inhibition zone was not much different from the inhibition zone in tetracycline antibiotic testing (positive control) which was 25 mm in S. aureus and 21.5 mm bacteria in E. coli bacteria. Tobacco essential oil from Probolinggo was able to inhibit the growth of S. aureus bacteria up to a concentration of 6.25% and E. coli bacteria to a concentration of 12.5%. Elda Nurnasari Kristiana Sri Wijayanti ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-17 2019-05-17 48 56 10.22435/jki.v9i1.1219 Uji Sensitisasi Dermal Masker Gel Kombinasi Ekstrak Ampas Daun Teh dan Air Cucian Beras https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/491 The combination of tea leaf dregs extract and rice washing water as a peel-off gel mask was found to be stable up to 28 days of storage and had an IC50 value 20.4 ppm. This potential can provide an optimal effects as cosmetic ingredients.However, to meet the regulatory requirements of Badan Pengawas Obat Makanan (BPOM) RI and ISO 10993, a dermal sensitization test also need to be done. The aim of this study was to determine the dermal sensitization reaction from peel-off gel mask containing a combination of tea leaf dregsextract (Camellia sinensis L.) and rice washing water (Oryza sativa L.) in test animal. Thisstudy was designed based on experimental laboratory methods and sample selection by simple random sampling. The intervention was given based on posttest-only control group design. The test results for dermal sensitization were determined using the Guinea Pig Maximization Test (GPMT) method. The results showed erythema and edema reactions according to the Magnusson and Kligman scale after administration of peel-off gel masks as samples in the topical tests in test animals, appeared on the 11th day and in the challenge test appeared on the 24th day. This was due to incubation period for one week where the hypersensitivity state can develop, so that this reaction is also called the delayed hypersensitivity reaction Andi Nurul Annisa Wahyu Utaminingrum Erza Genatrika ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-17 2019-05-17 57 64 10.22435/jki.v9i1.491 Kajian Sistematik: Efek Gen Multi Drug Resistance-1 pada Farmakokinetik Klopidogrel https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/584 Multi Drug Resistance-1 (MDR-1) gene polymorphisms encoding for P-glycoprotein can affect clopidogrel intestinal absorption. This systematic review aim to identify the impact of MDR-1 gene 3435 variants on clopidogrel pharmacokinetics. Literature review were retrieved from MEDLINE, Science Direct, Scopus, Clinical Key, ProQuest and Google Scholar databases. The articles are critically reviewed and analyzed to answer this systematic review’s aim. The result showed that, in patients with cardiovascular disease, the peak plasma concentration (Cmax) and the total area under the plasma concentration–time curve (AUC) of clopidogrel and its active metabolites were lower in 3435TT compared to 3435CC. Nevertheless, the variants of MDR-1 gene were not significantly correlated to the plasma concentration in healthy subjects. Clopidogrel pharmacokinetic profile varied widely between MDR-1 3435 variants and subjects. Rasmaya Niruri Rini Noviyani indah Mei Rahajeng ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-17 2019-05-17 65 72 10.22435/jki.v9i1.584 Back Matter Volume 9 No 1 Tahun 2019 https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/1818 Editor JKI ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2019-05-17 2019-05-17