Jurnal Kefarmasian Indonesia https://ejournal2.litbang.kemkes.go.id/index.php/jki <p>ISSN media elektronik:&nbsp;<a href="http://u.lipi.go.id/1387251248">2354-8770</a></p> <p>ISSN media cetak:&nbsp;<a href="http://u.lipi.go.id/1329660553">2085-675X</a></p> <p>Acredited by Ristekdikti Accreditation 21/E/KPT/2018 Sinta Rank Score S2 Valid until Volume 10 Number 2</p> <p>Jurnal Kefarmasian Indonesia is a scientific journal published by Center for Research and Development of Biomedical and Basic Health Technology, Board of Health Research and Development, Ministry of Health Republic of Indonesia (Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan, Badan Penelitian dan Pengembangan Kesehatan&nbsp;Kementerian Kesehatan Republik Indonesia). The journal publishes original research articles in pharmaceutical science such as Pharmaceutical Technology, Pharmacology, Pharmaceutical Chemistry, Traditional Medicines and Pharmaceutical Care. The journal is published in Bahasa Indonesia and English. Jurnal Kefarmasian Indonesia is a biannual, open access, peer-reviewed and online pharmacy journal. Jurnal Kefarmasian Indonesia aims to serve the updated scientific knowledge for processing manuscript as well as for processing manuscript.</p> <p>Jurnal Kefarmasian Indonesia has been indexed by <a href="https://doaj.org/toc/2354-8770?source=%7B%22query%22%3A%7B%22filtered%22%3A%7B%22filter%22%3A%7B%22bool%22%3A%7B%22must%22%3A%5B%7B%22term%22%3A%7B%22index.issn.exact%22%3A%222085-675X%22%7D%7D%2C%7B%22term%22%3A%7B%22index.issn.exact%22%3A%222354-8770%22%7D%7D%2C%7B%22term%22%3A%7B%22_type%22%3A%22article%22%7D%7D%5D%7D%7D%2C%22query%22%3A%7B%22match_all%22%3A%7B%7D%7D%7D%7D%2C%22from%22%3A0%2C%22size%22%3A100%7D">DOAJ</a>; <a href="http://isjd.pdii.lipi.go.id/index.php/public_no_login/index_direktori">Indonesian Scientific Journal Database</a>, <a href="http://id.portalgaruda.org/?ref=browse&amp;mod=viewjournal&amp;journal=4889">Indonesian Publication Index</a>, <a href="https://www.base-search.net/Record/1497b5f9cf595ef0ff1193d5afab7b2f432efcff10643f42eacaf41a5aa3aa83/">Bielefeld Academic Search Engine</a>, <a href="http://oaji.net/journal-detail.html?number=825">Open Academic Journal Index</a>; <a href="https://scholar.google.co.id/citations?user=gASqT4MAAAAJ&amp;hl=en">Google Scholar</a>; <a title="Sinta" href="http://sinta2.ristekdikti.go.id/journals/detail?id=2785">Sinta</a> Garda Rujukan Digital (<a href="http://garuda.ristekdikti.go.id/journal/view/4889">Garuda</a>). See Google Scholar Profile for Jurnal Kefarmasian Indonesia by clicking <a href="https://scholar.google.co.id/citations?user=gASqT4MAAAAJ&amp;hl=en">here</a>.</p> 14 articles of Jurnal Kefarmasian Indonesia has been cited by Scopus. Click <a href="https://drive.google.com/file/d/1hNSbYIoZqlp3Z7M14Ypw1zv-wYR8ElLV/view" target="_blank" rel="noopener">&nbsphere</a> for detail (update August 10, 2020). <p>Total citations: 514</p> <p>Total documents: 110</p> <p>h-index: 10&nbsp; &nbsp; &nbsp;i10-index: 13&nbsp; &nbsp; &nbsp;Impact Factor: 4,67</p> Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan en-US Jurnal Kefarmasian Indonesia 2085-675X Front Matter Volume 10 No 2 Tahun 2020 https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/3733 Editor JKI ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-27 2020-08-27 Potensi Ekstrak Etanol 96% Daun Salam (Syzigium polyanthum Wight. (Walp.)) terhadap Ekspresi p53 pada Sel Kanker HeLa Cell Lines https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/1996 Bay leaves (Syzigium polyanthum Wight. (Walp.) have been utilized as one of traditional medicines in Indonesian. However, the potency of the leaves for cancer treatment has not been well-explored. The aim of this study is to determine the expression of p53 tumor suppressor gene after administration of 96% ethanol extract of Bay leaves on HeLa cell lines. This study is begin by making extract using ethanol 96% as a solvent followed by phytochemical screening and extract evaluation of HeLa cells by immunohistochemistry. The observation of p53 protein expression is conducted using the Image J Profiler Plugin software 1.52a version. The H-score data is processed using the One-Way ANNOVA IBM SPSS 20 version program and continued with Post Hoc analysis using the Duncan test. The identification of 96% ethanol extract result showed that bay leaves contain flavonoids, alkaloids, saponins, phenols, terpenoids, and steroids. Based on the Post Hoc test results, the administration of 96% ethanol extract bay leaves with three dose variations (156 µg/ml, 234 µg/ml, 312 µg/ml) and negative control had significant differences, while the high dose (312 µg/ml) had not significant differences to positive control. The result showed that 96% ethanol extract of bay leaves by the dose of 312 ug/ml can inhibit the expression of mutant p53 protein in cervical cancer cells HeLa cell lines. Wahyu Hidayati Landyyun Rahmawan Sjahid Wahyu Ismalasari Kusmardi Kusmardi ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-24 2020-08-24 79 86 10.22435/jki.v10i2.1996 Karakteristik Minuman Probiotik Sari Ubi Kayu dari Kultur Bakteri Lactobacillus acidophilus dan Streptococcus thermophilus https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/2488 Functional foods are food that consumed, as usual, has a physiological effect and can reduce the effect of chronic disease. One of them is probiotic beverages. Probiotic beverages that have been widely known overtime was produced by a dairy house. This product could have been expensive due to the cost of raw materials. The alternative raw material such as the use of extracted cassava juice may reduce the price of the final product. The aims of this research were to study the effects of culture comparison between characteristics of the probiotic beverage from cassava juice, and get the best culture comparison that produces the best probiotic characteristics of the lactic beverage fermentation from cassava juice. This research applied qualitative experiment method in Lactobacillus acidophilus and Streptococcus thermophiles as an inoculum evaluated. Serial in culture ratio respectively 20:50; 30:50; 40:50; and 50:50. The observed research were pH value, viscosity, total lactic acid bacteria (LAB) and activities test against bacteria Escherichia coli. The result showed that the best culture comparison qualified as probiotics criteria was 50:50. It was having pH value 4,1; viscosity 0,706 g/mL; total LAB 2,1x106 cfu/mL and activity bacteria Escherichia coli was 5,3 mm. Nunung Yulia Adi Wibowo Eva Dania Kosasih ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-27 2020-08-27 87 94 10.22435/jki.v10i2.2488 Efek Iritasi Sabun Mandi Batang Mengandung Susu Produk Usaha Kecil Menengah di Kota Cimahi https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/2592 One of the ways to increase profit in small and medium business (UKM) in Cimahi is by diversifying its products, through manufacturing of bar soap derived from cow's milk from UKM. The requirement of bath soap is proper acidity (pH) level, otherwise it will make the skin dry, itchy or even increase irritation. Therefore, this in vivo non-clinical irritation evaluation is conducted to evaluate the effects of bar soap produced by UKM in Cimahi according to the guidelines in rat test animals. The animals is divided into five groups and exposed to soap for four hours. Five soap samples is obtained from UKM in Cipageran area, Cimahi. Observations is made at the hours of 1, 4, 24, 48, and 72 hours after exposure and is continued for 14 days. The measured parameters is erythema and edema, and the primary irritation index. The results showed that bar soap containing cow milk produced by UKM in Cimahi did not cause erythema and edema. Primary irritation index of all bath soap samples was 0.0 . It can be concluded that soap bars containing cow's milk produced by Cimahi City UKM including into the category of very mild irritation (negligigle). Suci Nar Vikasari Faizal Hermanto Elivas Simatupang Afifah Bambang Sutjiatmo Titta Hartyana Sutarna Ririn Puspadewi Lucky Rachmawan Fahmy Ahsanul Haq ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-24 2020-08-24 95 101 10.22435/jki.v10i2.2592 Perbandingan Teknik Aerasi dan Ultrasonikasi Gelasi Ionik Nanopartikel Deksametason Natrium Fosfat https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/2150 Alginate, a biocompatible and biodegradable natural polymer, has been widely used as a drug molecular carrier using ionic gelation methods (crosslinking). One of the factors that must be taken into account in its preparation is the mechanical effect. The purpose of this study was to explain the preparation process and the characteristics of the calcium alginate crosslinkers as dexamethasone sodium phosphate carriers with low energy and high energy techniques. Nanosuspension is made in six formulas using 3 techniques: low energy (aeration), high energy (ultrasonication), low and high energy (aeration and ultrasonication) with a fixed concentration of dexamethasone sodium phosphate and sodium alginate, that is 0,2% and 0,1%, with 0,02% and 0,2% of calcium chloride. Determination of particle size, zeta potential, and morphology of nanoparticles using Particle Size Analyzer (PSA) and Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM). Determination of encapsulation efficiency using UV/Vis spectrophotometer and statistical analysis using MANOVA test. Synthesis of nanosuspension using a combination of low and high energy (aeration and ultrasonication) results in the most optimal characteristics with particle size value of 352.90 ± 6.10 nm, homogenized polydispersity index (0,52 ± 0,04), optimal potential zeta -44,40 ± 0,4 mV, the encapsulation efficiency of 49,5 - 74,8% and spherical particle shape. It can be concluded that the preparation using a combination of low and high energy is the most optimal preparation result. Bambang Hernawan Nugroho Multi Tri Wardhani Suparmi Suparmi ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-27 2020-08-27 102 109 10.22435/jki.v10i2.2150 Optimasi Formula Krim Ekstrak Daun Katuk (Sauropus Androgynus) Variasi Konsentrasi Asam Stearat, Trietanolamin, dan Gliserin https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/2496 Sauropus androgynus (L.) Merr.) contain secondary metabolite compounds: flavonoids and polyphenols which has bioactivity as antioxidants. Antioxidants act as photoprotector, so it can be formulated into sun protection product. The selection of cream base is very important due to it’s not only as vehicle of active ingredients but also affecting cream’s physical properties and effectiveness. This study aims to determine the composition of the optimum base formula and evaluation formula cream of extract katuk leaves with variations concentration of stearic acid, TEA, and glycerin. Formula optimization was conducted through the Simplex Lattice Design methode with 13 formulas. The results of evaluation were processed with Design Expert software. The optimum formula was respectively at 13.16% stearic acid, 12% glycerin, and 3.84% TEA. The optimum formula cream evaluation was spreadability in 4.89 cm, 0.56 s stickiness power, and pH 6,1. Acceptability test results showed that the cream could accepted by society very well. Febia Arien Lestari Wahida Hajrin Nisa Isneni Hanifa ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-24 2020-08-24 110 119 10.22435/jki.v10i2.2496 Preparation of Curcumin-Liposomes using pH-Driven Method to Enhance Stability and Encapsulation Efficiency https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/2728 Curcumin, a hydrophobic polyphenol, derived from turmeric, has numerous pharmacological activities, including hepatoprotective, antioxidant, anti-inflammatory, antimicrobial, and anticarcinogenic. However, the therapeutic efficacy of curcumin is limited due to its poor oral bioavailability and high susceptibility to degradation. The poor oral bioavailability of curcumin has been attributed to its poor aqueous solubility and extensive first past metabolism. Curcumin dissolves in alkaline conditions but it is highly unstable undergoing rapid hydrolytic degradation in neutral or alkaline condition. To solve these problems, liposome-based encapsulation technology is applied to improve the bioavailability of curcumin. The existing method to prepare curcumin-liposome, thin-film method, is complex and usually required the use of organic solvents. In this study, pH-driven method, an organic solvent-free and easily encapsulation technique utilizing the pH-dependent solubility of curcumin was proposed to improve the stability and bioavailability. Curcumin-liposomes were prepared using phospholipon 90 H and soya lecithin as phospholipid sources. The characterizations of curcumin-liposomes include particle size, microstructure, encapsulation efficiency, infrared spectra, salt stability and storage stability. The particle size measured was 77.85 ± 0.39 nm. It was found that curcumin-liposomes prepared by the pH-driven method was stable during storage for 30 days and gave encapsulation efficiency of 60.71 ± 0.20%, higher as compared to the ones prepared by thin-film method. In conclusion, pH-driven is a promising method for the preparation of curcumin-liposomes to enhance stability and encapsulation efficiency Fransiska Christydira Sekaringtyas Suwasmi Purwajanti Etik Mardliyati Sri Ningsih ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-24 2020-08-24 120 129 10.22435/jki.v10i2.2728 Efektivitas Ekstrak Etanol Daun Durian (Durio zibethinus (Linn.)) sebagai Antihiperurisemia https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/2148 Durian (Durio zibethinus Linn.) leaves are known to inhibit the xanthine oxidase enzyme which can reduce uric acid levels in the blood. The purpose of study is to determine the effectiveness of ethanol extracts of durian leaves in reducing uric acid levels in hyperuricemic mice. The mice were induced with chicken’s liver juice 0,2 mL/20 gBW and potassium oxonate 250 mg/kgBW. The animals were mice with uric acid levels above 3,3 mg/dL or 6,2-7,1 mg/dL. The study was used a completely randomized design (CRD) with six treatments and three replications, with three mice in each replications. The treatments group were K+: Allopurinol 10 mg/kgBW; K-: CMC-Na 0.5%; extracts in group P1,P2, P3, P4 are 50; 100; 200; 250 mg/kgBW. The measurements of uric acid levels were performed on day-0, day-6 after induction, and day-15 after administering each group. The data obtained were analyzed by One Way Anova test followed by Duncan's Post Hoc further test. The results showed that durian leaves could reduce uric acid levels in mice blood with the highest percentage reduction was in P2 (100 mg/kgBW) group of 40,53%, followed by P1 (50 mg/kgBW) of 38,68%. The lowest percentage was in the P4 group (250 mg/kgBW) of 22,36%, followed by the P3 group (200 mg/kgBW) of 27,71% and the ED50 value was 61,65 mg/kgBW. In conclusion, ethanol extracts of durian leaves has a significant effect on decreasing total uric acid levels in mice and has potential as an antihyperuricemia agent. Rela Sonia Yusnelti Yusnelti Fitrianingsih Fitrianingsih ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-24 2020-08-24 130 139 10.22435/jki.v10i2.2148 Ekstrak Etanol Kopi Hijau Arabika (Coffea arabica L.) sebagai Antihiperglikemi pada Mencit (Mus musculus) https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/2122 Diabetes mellitus is an excessive level of blood sugar concentration disease. One of the compounds that can moderate the blood sugar level is chlorogenic acid that mostly found in green coffee. This study aims to determine the extract activity of Arabica (Coffea arabica L.) green coffee in decreasing the blood sugar level of mice (Mus musculus) that induced by alloxan monohydrate. The method is True Experimental Laboratories with the analysis of chlorogenic acid levels using a UV-Vis spectrophotometer. The research design is Post Test Control Group Design, using 24 mices that divided into six treatment groups. The groups consist of negative control (distilled water), Dose I (100 mg/kg bw), Dose II (200 mg/kg bw), Dose III (300 mg/kg bw), Dose IV (400 mg/kg bw), and positive control (metformin). Blood sugar levels of the mice is measured at the intervals of 1st day, 7th day, 21st day. The data is analyzed using the SPSS program with normality tests (Kolmogorov-Smirnov), homogeneity tests (Levene Test), and parametric statistical tests (One-Way ANOVA). The data results was normally distributed and homogeneous (P> 0.05) with several pairs of data in the treatment group were significantly different (P> 0.05). The extract of Arabica (Coffea arabica L.) green coffee contained 4.235% chlorogenic acid, could reduced the hyperglycemia mice blood sugar levels by the dose of 10.72% (Dose I); 19.85% (Dose II); 27.20% (Dose III); and 31.60% (Dose IV) with dose IV as the effective dose. Irawati Hamdani Salfauqi Nurman ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-24 2020-08-24 140 147 10.22435/jki.v10i2.2122 Back Matter Volume 10 No 2 Tahun 2020 https://ejournal2.litbang.kemkes.go.id/index.php/jki/article/view/3734 Editor JKI ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2020-08-27 2020-08-27