DETEKSI VIRUS HEPATITIS E (HEV) DAN HANTAVIRUS PADA INANG RESERVOIR (TIKUS) DI KABUPATEN KLATEN DAN KENDAL, PROVINSI JAWA TENGAH

  • Arief Mulyono Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit Salatiga
  • Tika Fiona Sari b2p2vrp
  • Ristiyanto Ristiyanto b2p2vrp
  • Bernadus Yuliadi b2p2vrp
  • Edi Royandi b2p2vrp
  • Ayu Pradipta Pratiwi b2p2vrp
Keywords: HEV, Hantavirus, Klaten, Kendal

Abstract

Rats are animal reservoirs and harbours of several zoonotic pathogens diseases in humans. At least, there are 68 viruses of zoonotic agents that can be transmitted by rats. Two common types of viruses attackinghumans are Hepatitis E virus (HEV) and Hantavirus. Early detection of those viruses is fundamentally required in order to prevent disease transmissions to humans. The aim of the study was to detect and count the percentage of rats infected by HEV and Hantavirus in Kendal and Klaten Districts, Central Java Province. The research design used in this study was descriptive design with cross-sectional approach. The target population was rats distributed in Klaten and Kendal Districts. In addition, the research subject was trapped rats. Detection of Hantavirus was carried out using ELISA method and detection of HEV was conducted using nested reverse transcription PCR (nested RT-PCR). A total of 73 rats was successfully captured consisting of 2 genus and 4 species e.g. Rattus novergicus, R. tanezumi, R. tiomanicus and Bandicota indica. The trapped rats infected by HEV were 3.7% and 41.3% for Klaten and Kendal, respectively. However, the seropositive of Hantavirus was only found in Kendal District (20.5%). Rat control is necessary to prevent transmission of HEV and Hantavirus.

References

Angela L, David H, Thomas S, Cryan P, Amy G, Pulliam J, et al., 2012. viruses: are bats special? A comparison of bats and rodents as reservoirs of zoonotic A comparison of bats and rodents as reservoirs of zoonotic viruses: are bats special? Proc R Soc B, p.9. Available at: http://rspb.royalsocietypublishing.org/content/suppl/2013/01/31/rspb.2012.2753.DC1.h%5Cnhttp://rspb.royalsocietypublishing.org/content/280/1756/20122753.full.html#ref-list-1%5Cnhttp://dx.doi.org/10.1098/rspb.2012.2753%5Cnhttp://rspb.royalsocietypublishing.
Blasdell K, Cosson JF, Chaval Y, Herbreteau V, Douangboupha B, Jittapalapong S, et al., 2011. Rodent-borne hantaviruses in Cambodia, Lao PDR, and Thailand. EcoHealth, 8(4), pp.432–443.
Ermonval M, Baychelier F & Tordo N, 2016. What do we know about how hantaviruses interact with their different hosts? Viruses, 8(8).
Guerra JA de AA, Kampa KC, Morsoletto DGB, Junior AP & Ivantes CAP, 2017. Hepatitis E: A Literature Review. Journal of Clinical and Translational Hepatology, X(X), pp.1–8.
He W, Wen Y, Xiong Y, Zhang M, Cheng M & Chen Q, 2018. The prevalence and genomic characteristics of hepatitis e virus in murine rodents and house shrews from several regions in China. BMC Veterinary Research, 14(1), pp.1–13.
Johansson P, Yap G, Low H-T, Siew C-C, Kek R, Ng L-C, et al., 2010. Molecular characterization of two hantavirus strains from different rattus species in Singapore. Virology journal, 7, p.15.
Johne R, Plenge-Bönig A, Hess M, Ulrich RG, Reetz J & Schielke A, 2010. Detection of a novel hepatitis E-like virus in faeces of wild rats using a nested broad-spectrum RT-PCR. Journal of General Virology, 91(3), pp.750–758.
Jonsson CB, Figueiredo LTM & Vapalahti O, 2010. A global perspective on hantavirus ecology, epidemiology, and disease. Clinical Microbiology Reviews, 23(2), pp.412–441.
Kamar N, Dalton HR, Abravanel F & Izopet J, 2014. Hepatitis E virus infection. Clinical Microbiology Reviews, 27(1), pp.116–138.
Kruger DH, Figueiredo LTM, Song JW & Klempa B, 2015. Hantaviruses-Globally emerging pathogens. Journal of Clinical Virology, 64, pp.128–136. Available at: http://dx.doi.org/10.1016/j.jcv.2014.08.033.
Li W, Guan D, Su J, Takeda N, Wakita T, Li TC, et al., 2013. High prevalence of rat hepatitis E virus in wild rats in China. Veterinary Microbiology, 165(3–4), pp.275–280. Available at: http://dx.doi.org/10.1016/j.vetmic.2013.03.017.
Manigold T & Vial P, 2014. Human hantavirus infections: Epidemiology, clinical features, pathogenesis and immunology. Swiss Medical Weekly, 144(March), pp.1–10.
Mulyanto, Depamede SN, Sriasih M, Takahashi M, Nagashima S, Jirintai S, et al., 2013. Frequent detection and characterization of hepatitis E virus variants in wild rats (Rattus rattus) in Indonesia. Archives of Virology, 158(1), pp.87–96.
Murphy EG, Williams NJ, Jennings D, Chantrey J, Verin R, Grierson S, et al., 2019. First detection of Hepatitis E virus ( Orthohepevirus C ) in wild brown rats ( Rattus norvegicus ) from Great Britain . Zoonoses and Public Health, (April), pp.1–9.
Pérez-Gracia MT, Suay-García B & Mateos-Lindemann ML, 2017. Hepatitis E and pregnancy: current state. Reviews in Medical Virology, 27(3), pp.1–8.
Plyusnina A, Ibrahim IN & Plyusnin A, 2009. A newly recognized hantavirus in the Asian house rat (Rattus tanezumi) in Indonesia. Journal of General Virology, 90(1), pp.205–209.
Reynes J-M, Carli D, Bour J-B, Boudjeltia S, Dewilde A, Gerbier G, et al., 2017. Seoul Virus Infection in Humans, France, 2014–2016. Emerging Infectious Diseases, 23(6), pp.973–977.
Ryll R, Bernstein S, Heuser E, Schlegel M, Dremsek P, Zumpe M, et al., 2017. Detection of rat hepatitis E virus in wild Norway rats (Rattus norvegicus) and Black rats (Rattus rattus) from 11 European countries. Veterinary Microbiology, 208, pp.58–68. Available at: http://dx.doi.org/10.1016/j.vetmic.2017.07.001.
Sridhar S, Yip CCY, Wu S, Cai J, Zhang AJX, Leung KH, et al., 2018. Rat hepatitis E virus as cause of persistent hepatitis after liver transplant. Emerging Infectious Diseases, 24(12), pp.2241–2250.
Suyanto A & Kartikasari SN, 2001. Kelelawar di Indonesia, Cibinong: Puslitbang Biologi LIPI.
Vaheri A, Strandin T, Hepojoki J, Sironen T, Henttonen H, Mäkelä S, et al., 2013. Uncovering the mysteries of hantavirus infections. Nature Reviews Microbiology, 11(8), pp.539–550. Available at: http://www.nature.com/doifinder/10.1038/nrmicro3066.
Zhang Y-Z, Zou Y, Fu ZF & Plyusnin A, 2010. Hantavirus Infections in Humans and Animals, China. Emerging Infectious Diseases, 16(8), pp.1195–1203.
Zupanc T, Saksida A & Korva M, 2015. Hantavirus infections. Clinical Microbiology and Infectious Diseases, pp.1–14.
Published
2019-09-25

Most read articles by the same author(s)