TEKNIK ISOLASI - IDENTIFIKASI Yersinia pestis SEBAGAI PENYEBAB PENYAKIT PES (HASIL PELATIHAN DI BALAI BESAR VETERINER BOGOR)
Abstract
Teknik isolasi dan identifikasi Y. pestis mempunyai prinsip- prinsip umum pertumbuhan yaitu terdiri dari tiga tahap yaitu: tahap pengkayaan, seleksi pada media agar dan uji biokimia. Tahap pengkayaan dilakukan dengan cara menimbang sebanyak 10-25 gram spesimen kemudian dimasukkan dalam blender atau plastik steril dan ditambah 90-225 ml media pengkayaan (dapat menggunakan Buffered Peptone Water (BPW), Brain Heart Infusion (BHI) atau menggunakan Nutrient Broth). Setelah itu dibuat suspensi spesimen 10%, kemudian dilakukan homogenisasi selama ± 2 menit dan diinkubasikan pada suhu 37 derajat Celcius selama 24 jam.
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