Kombinasi Minyak Kayu Putih dan Jeruk Nipis terhadap Mortalitas Pediculus humanus capitis

Janatin Nur Aripin; Dita Pratiwi Kusuma Wardani; M Luthfi Almanfaluthi; Isna Hikmawati

doi:10.22435/blb.v18i1.5357

Janatin Nur Aripin Program Studi Teknologi Laboratorium Medik D4, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Purwokerto, Jalan Letjen Soeparjdo Roestam KM 07. Po Box 2999 Purwokerto 53186, Jawa Tengah, Indonesia
Dita Pratiwi Kusuma Wardani Program Studi Teknologi Laboratorium Medik D4, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Purwokerto, Jalan Letjen Soeparjdo Roestam KM 07. Po Box 2999 Purwokerto 53186, Jawa Tengah, Indonesia
M Luthfi Almanfaluthi Program Studi Pendidikan Dokter, Fakultas Kedokteran Universitas Muhammadiyah Purwokerto, Jalan KH Ahmad Dahlan, Dukuhwaluh, Po Box 202 Purwokerto 53182, Jawa Tengah, Indonesia
Isna Hikmawati Program Studi Keperawatan, Fakultas Ilmu Kesehatan, Universitas Muhammadiyah Purwokerto, Jalan Letjen Soeparjdo Roestam KM 07. Po Box 2999 Purwokerto 53186, Jawa Tengah, Indonesia

DOI: https://doi.org/10.22435/blb.v18i1.5357

Keywords: lime, cajuput oil, Pediculus humanus capitis

Abstract

The use of synthetic chemical pediculicides is known to have side effects and the occurrence of resistance so plant-based pediculicides are needed to kill Ph. capitis. Eucalyptus and lime oil are known to have an effect in killing Ph. capitis. The purpose of this study was to determine the effectiveness of the combination of eucalyptus and lime oil on the mortality of Ph. capitis. This study was conducted in a true experimental with a post-test-only control group design in May 2021. This study consisted of 5 treatment groups, one group as a positive control (permethrin 1%), one group as a negative control (aquadest), and 3 groups received treatment with a combination of eucalyptus and lime oil in the proportions of 1:3 (25 ml of eucalyptus oil: 75 ml of lime juice), 1:1 (50 ml of eucalyptus oil: 50 ml of lime juice) and 3: 1 (75 ml eucalyptus oil: 25 ml lime juice). It was repeated 3 times so that the total sample was 150 Ph. capitis which was observed every 5 minutes for 1 hour. The research data were analyzed using the Kruskal-Wallis test and continued with the Posthoc Mann-Whitney U test. Mortality of Ph. capitis in the combination group the most effective was the combination of 75% eucalyptus oil and 25% lime oil in the 5th-minute observation of 100% mortality compared to the control group and other groups.

References

1. Hayati I. Mortalitas Pediculus humanus capitis terhadap air perasan Citrus histrix. J Ilm AVICENNA. 2019;14(3):52-56.

2. Coates SJ, Thomas C, Chosidow O, Engelman D, Chang AY. Ectoparasites: Pediculosis and tungiasis. J Am Acad Dermatol. 2020;82(3):551-569. doi:10.1016/j.jaad.2019.05.110.

3. Deeksha, Malviya R, Sharma PK, Singh D, Sharma A. Formulation of herbal shampoo against head louse (Pediculus humanus capitis De Geer). Open Biol J. 2021;8(1):74-80. doi:10.2174/1874196702008010074.

4. Sayekti FDJ, Qurrohman MT, Priyandari DA, Srikandini C. Pengaruh kombinasi buah jeruk nipis dan buah mengkudu terhadap mortalitas Pediculus humanus capitis. At-Taqaddum. 2020;12(1):47-54.

5. Nurmatialila W, Widyawati, Utami A. Hubungan antara tingkat pengetahuan mengenai pedikulosis kapitis dan praktik kebersihan diri dengan kejadian pedikulosis kapitis pada siswa SDN 1 Tunggak Kecamatan Toroh Kabupaten Grobogan. Diponegoro Med J (Jurnal Kedokt Diponegoro). 2019;8(3):1081-1091.

6. Kristinawati E, Zaetun S, Srigede L. Efektivitas kombinasi filtrat daun jeruk nipis (Citrus aurantifolia) dan daun pandan wangi (Pandanus amaryllifolius) sebagai insektisida alami pembasmi kutu rambut (Pediculus humanus capitis). Media Bina Ilm. 2018;1(1):7-12.

7. Anggraini A, Anum Q, Masri M. Hubungan Tingkat pengetahuan dan personal hygiene terhadap kejadian pedikulosis kapitis pada anak asuh di panti asuhan liga dakwah Sumatera Barat. J Kesehat Andalas. 2018;7(1):131. doi:10.25077/jka.v7.i1.p131-136.2018.

8. Arrizqiyani T, Khusnul, Virgianti DP. Uji efektivitas formula Pedikulosida berbahan aktif minyak atsiri terhadap mortalitas kutu kepala (Pediculus humanus capitis De Geer) secara in vitro. J Muhammadiyah Med Lab Technol. 2018;2(1):1-11.

9. Imboden A. Effective treatments for head lice. Nurse Pract. 2019;44(9):36-42. doi:10.1097/01.NPR.0000574668.19239.db.

10. Leung AKC, Lam JM, Leong KF, Barankin B, Hon KL. Paediatrics : how to manage pediculosis capitis. Drugs Context. 2021;11:1-15.

11. Husni L, Al-Waiz M. Topical ivermectin in the treatment of pediculosis capitis. Our Dermatology Online. 2021;12(1):14-18. doi:10.7241/ourd.20211.3.

12. Candy K, Nicolas P, Andriantsoanirina V, Izri A, Durand R. In vitro efficacy of five essential oils against Pediculus humanus capitis. Parasitol Res. 2018;117(2):603-609. doi:10.1007/s00436-017-5722-5.

13. Arserim SK, Cetin H, Yildirim A, et al. The Toxicity of essential oils from three origanum species against head louse, Pediculus humanus capitis. Acta Parasitol. 2021;66(3):1003-1011. doi:10.1007/s11686-021-00370-y.

14. Candy K, Akhoundi M, Andriantsoanirina V, Durand R, Bruel C, Izri A. Essential oils as a potential treatment option for pediculosis. Planta Med. 2020;86(9):619-630. doi:10.1055/a-1161-9189.

15. Susanty E, Lesmana SD, Afandi D, Yulianto R, Andhika KR. Uji efektivitas Citrus limon dan Allium sativum sebagai pedikulisida secara In Vitro. J Kedokt Brawijaya. 2020;31(2):89-94.

16. Sittichok S, Wongnet O, Soonwera M. New Thai herbal shampoos as pediculicides for killing head louse, Pediculus humanus capitis de Geer (Phthiraptera). Asian Pac J Trop Biomed. 2018;8(2):106-112. doi:10.4103/2221-1691.225614.

17. Al-Zayyadi SW. Study of the effectiveness of some raw plants and materials in the treatment of pediculosis in Najaf Province Iraq. Indian J Forensic Med Toxicol. 2020;14(1):499-503. doi:10.37506/v14/il/2020/ijfmt/192948.

18. Robby AF, Pratama IS, Tresnani G. Pediculicidal Activity of Cem-ceman White Cempaka Flower (Michelia alba DC.) against Pediculus humanus capitis. ad-Dawaa’ J Pharm Sci. 2021;4(1). doi:10.24252/djps.v4i1.21154.

19. Purdiani DA, Wardani DPK, Sulistyowati R. Efektivitas ekstrak bawang putih (Allium sativum L.) dan cuka apel terhadap mortalitas Pediculus humanus. J Labora Med. 2021;5:1-7.

20. Sittichok S, Chantawee A, Soonwera M. Efficacy of Thai herbal shampoos from Averrhoa carambola L., Hibiscus sabdariffa L. and Passiflora edulis Sims. for controlling head lice, Pediculus humanus capitis (De Geer). Int J Agric Technol. 2018;14(5):751-766.

21. Hayati I. Mortalitas Pediculus humanus capitis terhadap air perasan Citrus histrix. Avicenna: J Ilm. 2017;13(3):2628-2637.

22. Kartini S, Hendrika Y, Wahyudiani R. Uji efektifitas ekstrak etanol daun jarak pagar (Jatropha curcas L.) terhadap mortalitas kutu kepala (Pediculus humanus capitis). J Pharm Sci. 2021;5(1):35-40.

23. Benelli G, Pavela R. Beyond mosquitoes-essential oil toxicity and repellency against bloodsucking insects. Ind Crops Prod. 2018;117(November 2017):382-392. doi:10.1016/j.indcrop.2018.02.072.

24. Putri WM, Pradana MS, Suryanto I. Potensi kombinasi sirih merah dan daun srikaya sebagai alternatif bahan alami anti kutu rambut (Pediculus humanus capitis). J Sain Heal. 2020;4(2):27-32.

25. Soonwera M, Wongnet O, Sittichok S. Ovicidal effect of essential oils from Zingiberaceae plants and Eucalytus globulus on eggs of head lice, Pediculus humanus capitis De Geer. Phytomedicine. 2018;47:93-104. doi:10.1016/j.phymed.2018.04.050.

26. Virgianti DP, Rahmah LA. Efektifitas beberapa minyak kayu putih terhadap mortalitas Pediculus humanus capitis secara in vitro. J Kesehat Bakti Tunas Husada. 2016;15(1):10-18.

27. Suprobowati OD, Suliati. Efek perasan jeruk nipis (Citrus aurantifolia) terhadap mortalitas kutu kepala (Pediculus humanus var . capitis) secara in vitro. In: Seminar Nasional Kesehatan Tahun 2016. Politeknik Kesehatan Kemenkes Surabaya; 2016:86-89.

28. Rivai H, Asra R, Nurtrismawati. Analisis fitokimia dari ramuan obat tradisional untuk nyeri haid: jeruk nipis (Citrus aurantifolia (Christm.) Swingle). ResearchGate. 2020;(April). doi:10.13140/RG.2.2.23826.40641.

29. Greive KA, Barnes TM. The efficacy of Australian essential oils for the treatment of head lice infestation in children : a randomised controlled trial. Australas J of Dermatology. 2018;59:e99-e105. doi:10.1111/ajd.12626.

30. Torry FR, Syarifuddin I. Pemetaan kualitas minyak kayu putih (Melaleuca leucadendra) di Maluku. Maj Biam. 2016;12(01):14-19.

31. Hayati I, Nopitasari H. Uji efektivitas air perasan jeruk nipis (Citrus limon) dan jeruk lemon (Citrus aurantifolia) terhadap mortalitas kutu kepala (Pediculus humanus capitis). J Ilm Pharm. 2020;7(1):26-32. doi: 10.52161/jiphar.v7i1.104.

32. Sittichok S, Soonwera M. Efficacy of new herbal shampoos from Garcinia dulcis kurz, Citrus aurantium l. and Eucalyptus globulus labill as pediculicides for head lice (Pediculus humans capitis) control. Int J Agric Technol. 2018;14(4):597-612.

33. Putri WM, Pradana MS, Suryanto I. Potensi kombinasi sirih merah dan daun srikaya sebagai alternatif bahan alami anti kutu rambut (Pediculus humanus capitis). J Sain Heal. 2020;4(2):27-32.

34. Fang F, Candy K, Melloul E, et al. In vitro activity of ten essential oils against Sarcoptes scabiei. Parasites and Vectors. 2016;9(1):1-7. doi:10.1186/s13071-016-1889-3.