PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG  DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK  IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10

Dyah Widiastuti; Sitti Rahmah Umniyati; Nastiti Wijayanti

doi:10.22435/blb.v8i1.782

PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10

Dyah Widiastuti
Sitti Rahmah Umniyati
Nastiti Wijayanti

DOI: https://doi.org/10.22435/blb.v8i1.782

Keywords: Dengue, immunocytochemistry, DSSE10

Abstract

Dengue viruses, globally the most prevalent arboviruses, are transmitted to humans by persistently infected
Aedes mosquitoes. The most important vector of Dengue virus is the mosquito Ae.aegypti, which should be the main target
of surveillance and control activities. Virologic surveillance for dengue viruses in its vector has been used as an early
warning system to predict outbreaks. Detection of Dengue virus antigen in mosquito head squash using
immunocytochemical streptavidin biotin peroxidase complex (SBPC) assay is an alternative method for dengue vector
surveillance. The study aimed to develope immunocytochemical SBPC assay to detect Dengue virus infection in head
squash of Ae.aegypti. The study design was experimental. Artificially-infected adult Ae. aegypti mosquitoes of DENV 3
were used as infectious samples and non-infected adult Ae. aegypti mosquitoes were used as normal ones. The
immunocytochemical SBPC assay using monoclonal antibody DSSE10 then was applied in mosquito head squash to
detect Dengue virus antigen. The results were analyzed by descriptive analysis. The immunocytochemical SBPC assay
can detect Dengue virus antigen in mosquito head squash at day 2 postinfection. There are some false positive results
found in immunocytochemical SBPC assay.

Published

2012-06-01

How to Cite

Widiastuti D, Umniyati S, Wijayanti N. PEMERIKSAAN VIRUS DENGUE-3 PADA NYAMUK Aedes aegypti YANG DIINFEKSI SECARA INTRATHORAKAL DENGAN TEKNIK IMUNOSITOKIMIA MENGGUNAKAN ANTIBODI DSSE10. blb [Internet]. 1Jun.2012 [cited 8May2024];8(1):21-5. Available from: http://ejournal2.litbang.kemkes.go.id/index.php/blb/article/view/782

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Volume 8 Nomor 1 Juni 2012

Section

Articles

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

The Authors submitting a manuscript do so on the understanding that if accepted for publication, the copyright of the article shall be assigned to BALABA .
Copyright encompasses exclusive rights to reproduce and deliver the article in all forms and media, including reprints, photographs, microfilms, and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases, and its transmission by any form or media, such as electronic, electrostatic, and mechanical copies, photocopies, recordings, magnetic media, etc., will be allowed only with written permission from BALABA.
BALABA, the Editors, and the Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in this journal.