PEMERIKSAAN BAKTERI LEPTOSPIRA PADA SAMPEL DARAH MANUSIA SUSPECT LEPTOSPIROSIS MENGGUNAKAN METODE PCR (POLYMERASE CHAIN REACTION)
Abstract
PEMERIKSAAN BAKTERI LEPTOSPIRA PADA SAMPEL DARAH MANUSIA SUSPECT LEPTOSPIROSIS MENGGUNAKAN METODE PCR (POLYMERASE CHAIN REACTION)
Abstract
ABSTRACT
Leptospirosis is a zoonotic disease, which is caused by leptospira. Leptospirosis cases often show no specific
clinical symptoms and is difficult to diagnose without testing samples in the laboratory. Testing using PCR
(Polymerase Chain Reaction) is considered more accurate than the other methods. Components required in the
examination Leptospira bacteria in human blood samples using PCR method is DNA template, DNA polymerase
enzyme, forward primer (PU1 and SU1) and reverse primer (Lep R1), nuclease free water, Mg 2 +, and dNTPs.
Examination of Leptospira bacteria in human blood samples include sampling, DNA isolation, examination by
PCR, and electrophoresis running.
Key words: leptospirosis, Leptospira, PCR methods
ABSTRAK
Leptospirosis adalah penyakit zoonosis yang disebabkan oleh bakteri Leptospira. Kasus leptospirosis sering
tidak menunjukkan gejala klinis yang spesifik dan sulit didiagnosis tanpa pengujian sampel di laboratorium.
Pengujian dengan menggunakan metode PCR (Polymerase Chain Reaction) dinilai lebih akurat dibandingkan
dengan metode yang lain. Komponen-komponen yang dibutuhkan dalam pemeriksaan bakteri Leptospira pada
sampel darah manusia menggunakan metode PCR adalah DNA template, enzim polymerase, Primer PU 1 dan
Primer SU 1, Primer Lep R1, air, Mg2+ , dan dNTP. Pemeriksaan bakteri Leptospira pada sampel darah manusia
meliputi pengambilan sampel, isolasi DNA, pemeriksaan dengan metode PCR, dan running elektroforesis.
Kata kunci: leptospirosis, Leptospira, metode PCR
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