The prediction of Influenza A/H3N2 Vaccine Efficacy using samples obtained from Indonesian Hajj pilgrims in 2013

Agustiningsih Agustiningsih; Kartika Dewi Puspa; Hartanti Dian Ikawati; Eka Pratiwi; Ririn Ramadhany; rene Lorinda Indalao; Krisna N A Pangesti

doi:10.22435/hsji.v9i1.461

Agustiningsih Agustiningsih National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
Kartika Dewi Puspa National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
Hartanti Dian Ikawati National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
Eka Pratiwi National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
Ririn Ramadhany National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
rene Lorinda Indalao National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia
Krisna N A Pangesti National Institute of Health Research and Development, Ministry of Health, Jakarta, Indonesia

DOI: https://doi.org/10.22435/hsji.v9i1.461

Keywords: Vaccine efficacy, influenza A/H3N2 virus, Hajj pilgrim, Indonesia

Abstract

Abstrak

Latar Belakang: Vaksinasi merupakan salah satu cara efektif dalam mengontrol dan mengurangi beban penyakit yang disebabkan oleh Influenza. Akan tetapi, efikasi vaksin bisa bervariasi jika strain yang digunakan untuk vaksin berbeda dengan strain yang bersirkulasi di dunia. Hal ini menunjukan pentingnya melakukan analisa prediksi efikasi vaksin. Pada studi ini, prediksi efikasi vaksin Influenza A/H3N2 dilakukan berdasarkan perhitungan antigenic distance strain vaksin WHO dengan virus influenza yang berasal dari jemaah Haj iIndonesia pada tahun 2013.

Metode: Sekuensing gen HA dilakukan terhadap dua sampel tersimpan yang terkonfirmasi positif Influenza A/ H3N2 yang berasal dari jemaah Haji Indonesia tahun 2013. Pepitope Calculator digunakan untuk menghitung antigenic distance dari dua strain virus influenza dan dilanjutkan dengan perhitungan Pepitope value. Vaksin strain yang direkomendasikan oleh WHO; A/Texas/50/2012, A/Switzerland/9715293/2013, A/HongKong/4801/2014 dan dua virus yang diambil dari jemaah Haji Indonesia pada tahun 2013 dianalisa pada studi ini.

Hasil: Prediksi efikasi vaksin yang direkomendasikan WHO tahun 2013 (A/Texas/50/2012) dengan sampel yang berasal dari jemaah Haji Indonesia tahun 2013 menunjukkan hasil lebih rendah dibandingkan dengan strain vaksin untuk musim flu pada tahun selanjutnya. Hasil ini sesuai dengan hasil analisis filogenetik dan perbandingan asam amino dimana sampel pada studi ini berkerabat lebih dekat dengan strain vaksin untuk musim flu selanjutnya dengan perbedaan asam amino yang lebih sedikit di bagian epitope protein HA dibandingkan dengan vaksin tahun 2013.

Kesimpulan: Perhitungan efikasi vaksin menggunakan antigenic distance antara strain vaksin WHO dan virus yang menginfeksi jemaah haji Indonesia pada tahun 2013 menunjukkan hasil yang rendah. (Health Science Journal of Indonesia 2018;9(1):1-7)

Keywords: Efikasi vaksin, Influenza A/H3N2, jemaah Haji, Indonesia

Abstract

Background: Influenza vaccination is an effective approach to control and reduce the disease burden of influenza viruses. However, the efficacy of influenza vaccine varies every year due to the different antigenic distance between vaccine and the circulating influenza strains globally and therefore necessitates the study of vaccine efficacy (VE). This study describes the prediction of Influenza A/H3N2 VE based on antigenic distances WHO vaccine strains and the virus obtained from Indonesian Hajj pilgrims in 2013.

Methods: Coding between Sequence of HA gene of Influenza A/H3N2 virus was obtained from archival samples of Indonesian Hajj Pilgrims in 2013. Pepitope value calculation using Pepitope Calculator to measure the antigenic distance of HA sequences of two influenza strains was implemented. The HA sequences of WHO vaccine strains: A/ Texas/50/2012, A/Switzerland/9715293/2013, A/HongKong/4801/2014 and two influenza viruses from Indonesian Hajj pilgrims in 2013 were analyzed.

Results: This study predicted that influenza vaccine strain recommended by WHO for 2013 (A/Texas/50/2012) have low efficacy to the influenza virus obtained from Indonesian Hajj Pilgrim in 2013 while showing higher efficacy to vaccine strain recommended for the following year. This result was in line with phylogenetic analysis and amino acid differences in which the samples in this study were grouped together with vaccine strain in following years and had less amino acid differences in epitope located in HA protein compared with 2013 vaccine strain.

Conclusion: The prediction of VE using the antigenic distance measurement between WHO vaccine strain and Indonesian Hajj pilgrim collected in 2013, is considered low. (Health Science Journal of Indonesia 2018;9(1):1-7)

Keywords: Vaccine efficacy, influenza A/H3N2 virus, Hajj pilgrim, Indonesia

References

WHO. Influenza virus activity in the world. http://www.who.int/influenza/gisrs_laboratory /updates/summaryreport/en/index.html. Accessed 20 December 2011.

Holmes EC. Evolutionary history and phylogeography of human viruses. Annu Rev Microbiol. 2008;62:307-328.

Rambaut A, Pybus OG, Nelson MI, Viboud C, Taubenberger JK, Holmes EC. The genomic and epidemiological dynamics of human influenza A virus. Nature. May 29 2008;453(7195):615-619.

Fiore AE, Uyeki TM, Broder K, et al. Prevention and control of influenza with vaccines: recommendations of the Advisory Committee on Immunization Practices (ACIP), 2010. MMWR Recomm Rep. Aug 6 2010;59(RR-8):1-62.

Falchi A, Amoros JP, Arena C, et al. Genetic structure of human A/H1N1 and A/H3N2 influenza virus on Corsica Island: phylogenetic analysis and vaccine strain match, 2006-2010. PLoS One. 2011;6(9):e24471.

Gupta V, Earl DJ, Deem MW. Quantifying influenza vaccine efficacy and antigenic distance. Vaccine. May 1 2006;24(18):3881-3888.

Colman PM, Varghese JN, Laver WG. Structure of the catalytic and antigenic sites in influenza virus neuraminidase. Nature. May 5-11 1983;303(5912):41-44.

Prevention CfDCa. Influenza Activity: United States and worldwide, 2007-2008 Season. MMWR Morb Mortal Wkly Rep. 27 June 2008 2008;57(25):692-697.

Alzeer AH. Respiratory tract infection during Hajj. Ann Thorac Med. Apr 2009;4(2):50-53.

Benkouiten S, Charrel R, Belhouchat K, et al. Circulation of respiratory viruses among pilgrims during the 2012 Hajj pilgrimage. Clin Infect Dis. Oct 2013;57(7):992-1000.

Barr IG, McCauley J, Cox N, et al. Epidemiological, antigenic and genetic characteristics of seasonal influenza A(H1N1), A(H3N2) and B influenza viruses: basis for the WHO recommendation on the composition of influenza vaccines for use in the 2009-2010 Northern Hemisphere season. Vaccine. Feb 3 2010;28(5):1156-1167.

Alfelali M, Khandaker G, Booy R, Rashid H. Mismatching between circulating strains and vaccine strains of influenza: Effect on Hajj pilgrims from both hemispheres. Hum Vaccin Immunother. Mar 03 2016;12(3):709-715.

WHO. WHO Global Influenza Surveillance Network: Manual for the laboratory diagnosis and virological surveillance of influenza; 2011.

Agustiningsih A, Trimarsanto H, Setiawaty V, Artika IM, Muljono DH. Primer development to obtain complete coding sequence of HA and NA genes of influenza A/H3N2 virus. BMC Res Notes. 2016;9(1):423.

Munoz ET, Deem MW. Epitope analysis for influenza vaccine design. Vaccine. Jan 19 2005;23(9):1144-1148.

Deem MW. Calculation of Pepitope using Microsoft Excel http://www.mwdeem.rice.edu/pepitope/.

Chutinimitkul S, Chieochansin T, Payungporn S, et al. Molecular characterization and phylogenetic analysis of H1N1 and H3N2 human influenza A viruses among infants and children in Thailand. Virus Res. Mar 2008;132(1-2):122-131.

Deem MW, Pan K. The epitope regions of H1-subtype influenza A, with application to vaccine efficacy. Protein Eng Des Sel. Sep 2009;22(9):543-546.

Pan K, Subieta KC, Deem MW. A novel sequence-based antigenic distance measure for H1N1, with application to vaccine effectiveness and the selection of vaccine strains. Protein Eng Des Sel. Mar 2010;24(3):291-299.

Holmes EC, Grenfell BT. Discovering the phylodynamics of RNA viruses. PLoS Comput Biol. Oct 2009;5(10):e1000505.

Ghedin E, Fitch A, Boyne A, et al. Mixed infection and the genesis of influenza virus diversity. J Virol. Sep 2009;83(17):8832-8841.

Hobson D, Curry RL, Beare AS, Ward-Gardner A. The role of serum haemagglutination-inhibiting antibody in protection against challenge infection with influenza A2 and B viruses. J Hyg (Lond). Dec 1972;70(4):767-777.

Benoit A, Beran J, Devaster JM, et al. Hemagglutination Inhibition Antibody Titers as a Correlate of Protection Against Seasonal A/H3N2 Influenza Disease. Open Forum Infect Dis. Apr 2015;2(2):ofv067.

Beyer WE, Palache AM, de Jong JC, Osterhaus AD. Cold-adapted live influenza vaccine versus inactivated vaccine: systemic vaccine reactions, local and systemic antibody response, and vaccine efficacy. A meta-analysis. Vaccine. Jan 31 2002;20(9-10):1340-1353.

Kosasih H, Roselinda, Nurhayati, et al. Surveillance of Influenza in Indonesia, 2003-2007. Influenza Other Respi Viruses. Jul 13 2012.

Wang TT, Palese P. Universal epitopes of influenza virus hemagglutinins? Nat Struct Mol Biol. Mar 2009;16(3):233-234.

Wilson IA, Cox NJ. Structural basis of immune recognition of influenza virus hemagglutinin. Annu Rev Immunol. 1990;8:737-771.

Tewawong N, Suntronwong N, Vichiwattana P, Vongpunsawad S, Theamboonlers A, Poovorawan Y. Genetic and antigenic characterization of hemagglutinin of influenza A/H3N2 virus from the 2015 season in Thailand. Virus Genes. Oct 2016;52(5):711-715.

WHO. Review of the 2014-2015 influenza season in the northern hemisphere. Wkly Epidemiol Rec. Jun 05 2015;90(23):281-296.

Wiley DC, Wilson IA, Skehel JJ. Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation. Nature. Jan 29 1981;289(5796):373-378.