Potensi Ekstrak Daun Marigold (Tagetes erecta L.) sebagai Larvasida terhadap Larva Aedes aegypti di Laboratorium
Potential Extract of Marigold (Tagetes erecta L.) Leaves as Larvacide Against Aedes aegypti Larvae in the Laboratory
Abstract
Abstract
Control of mosquito vectors by using natural ingredients is being developed intensively. Marigold (Tagetes erecta) was widely used as a mosquito repellent plant. The results of research before had found that marigold leaf extract has a repulsive effect on Aedes aegypti mosquitoes. This study aimed to identify the potential of marigold leaf extract as larvacide against Ae. aegypti larvae in the laboratory. The material used in this research was extracts of marigold leaves. Larvacide test was carried out by dissolving of extract in water at the concentration of 2,000 ppm, 4,000 ppm, 6,000 ppm, 8,000 ppm, and 10,000 ppm. The larvae used was Ae. aegypti larvae as many as 25 larvae each five treatment of replications. Probit analysis was conducted on larval mortality after 24 hours of exposure. The results of the probit analysis showed that the estimated concentration of extracts that could result in larval mortality up to 95% (LC95) was 7,456 ppm. From the result we can conclude that ethanol extract of marigold leaves (T. erecta L. ) has the biolarvacidal activity against larvae.
Abstrak
Pengendalian vektor nyamuk dengan memanfaatkan bahan alam makin banyak dikembangkan. Marigold (T. erecta) merupakan tumbuhan yang banyak dimanfaatkan sebagai tanaman pengusir nyamuk. Hasil penelitian sebelumnya menemukan bahwa ekstrak daun memiliki daya tolak terhadap nyamuk Ae. aegypti. Penelitian ini bertujuan untuk melihat potensi ekstrak daun marigold sebagai larvasida terhadap larva Ae. aegypti instar III di laboratorium. Bahan yang digunakan pada penelitian ini adalah ekstrak etanol daun marigold hasil ekstraksi dari penelitian sebelumnya. Uji larvasida dilakukan dengan melarutkan ekstrak dalam air pada konsentrasi 2.000 ppm, 4.000 ppm, 6.000 ppm, 8.000 ppm, dan 10.000 ppm. Larva yang digunakan yaitu larva Ae. aegypti sebanyak 25 larva tiap perlakuan dengan lima ulangan. Dilakukan analisis probit terhadap kematian larva setelah 24 jam paparan. Hasil analisa probit menunjukkan perkiraan konsentrasi ekstrak yang mampu mengakibatkan kematian larva hingga 95% (LC95 ) adalah 7.456 ppm. Ekstrak etanol daun marigold (T.erecta L.) memiliki potensi sebagai biolarvasida terhadap larva Ae. aegypti.
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